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表面超微结构     
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  surface ultrastructure
     The changes of the growth characteristics and surface ultrastructure during longterm passages of Wg3h cells that have been transfected with PSV_2-neo (Wg3h-neo) were studied.
     本文对转染PSV_2-neo质粒后的Wg3h细胞系(Wg3h-neo)在长期传代中的生长特性和表面超微结构与母系Wg3h细胞进行了比较研究。
短句来源
     Topographical observation of apoptosis and membrane surface ultrastructure of PC12 cells
     PC12细胞凋亡及其细胞膜表面超微结构的原子力显微镜形态学观察
短句来源
     SURFACE ULTRASTRUCTURE OF HUMAN PROMYELOCYTIC LEUKEMIA CELLS(HL-60)AND CHANGES DURING INDUCED DIFFERENTIATION
     人早幼粒白血病细胞(HL-60)的表面超微结构及其在诱导分化过程中的变化
短句来源
     A STUDY ON THE CHANGES OF GROWTH CHARACTERISTICS AND SURFACE ULTRASTRUCTURE OF MG3H BEFORE AND AFTER TRANSFECTION WITH PSV2-NEO PLASMID
     Wg3h细胞系转染PSV_2-neo质粒前后生长特性及表面超微结构改变的研究
短句来源
     Surface ultrastructure and fluoride distribution of enamel in laser-NaF treated group, laser treated group, NaF treated group and control group were observed by scanning electron microscope (SEM) and electron probe microanalyzer.
     扫描电镜 (SEM)、电子探针分别观察和分析激光加氟化钠组、激光组、氟化钠组、空白组 (不处理 )牙釉质表面超微结构及牙釉质内部氟离子分布水平。
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  surface structures
     In situ observation of surface structures of aorta and aorta valve endothelial cells with AFM
     利用AFM原位观察兔主动脉和主动脉瓣膜内皮细胞表面超微结构
短句来源
     Effects of high cholesterol diet and parallel atorvastatin treatment on the surface structures of aortic valve endothelial cells
     高脂饮食及阿托伐他汀干预对主动脉瓣膜内皮细胞表面超微结构的影响
短句来源
     For the first time, the ultramicroscopic surface structures of esophageal squ-amous cells of different grades. including normal. hyperplasia, severe dysplasia-grade Ⅰ and Ⅱ near-carcinoma and carcinoma, in the balloon smears of 22 cawere studied under scanning electron microscope.
     应用扫描电镜技术观察了22例在河南林县取材的食管拉网涂片中,经光镜定性、定位的正常、轻度增生、重度增生Ⅰ级、重度增生Ⅱ级、近癌和癌变鳞状上皮细胞的表面超微结构的变化。
短句来源
     Objective To observe the surface structures of cardiovascular endothelial cells in situ with atom force microscope(AFM).
     目的利用原子力显微镜(AFM)原位观察正常新西兰白兔主动脉和主动脉瓣膜内皮细胞表面超微结构
短句来源
  cell surface ultramicrostructure
     EFFECTS OF DMSO ON THE CELL SURFACE ULTRAMICROSTRUCTURE AND C-myc GENE OF HL-60 CELL
     二甲基亚砜对HL-60细胞表面超微结构及C-myc基因的影响
短句来源
     Changes of HL-60 cell surface ultramicrostructure and amplified structure DM of C-myc gene during differentiation induced by DMSO were studied.
     本文应用电子显微镜和染色体分析技术研究了二甲基亚砜(DMSO)诱导HL-60细胞分化过程中,细胞表面超微结构和C-myc基因扩增结构的变化。
短句来源
  surface ultra
     The results showed that each group had its characteristics of cell outline and series of cell surface ultra struetural changes appeared In different stages of cultured and various functional stages.
     结果:随培养时间和机能状态不同,五组细胞呈不同的外形和一系列表面超微结构变化特征。
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  surface ultrastructure
Surface Ultrastructure of the Heteromorphic Cells of Nostoc muscorumCALU 304 in a Mixed Culture with the RauwolfiaCallus Tissue
      
The in vitro effect of pirprofen on the surface ultrastructure and function of human platelets
      
The in vitro effect of pirprofen (Rengasil), an antiinflammatory agent, on the surface ultrastructure and function of human platelets was examined and compared with that of acetylsalicylic acid (Aspirin, ASA), and diclofenac sodium (Voltaren, DS).
      
Surface ultrastructure of the human placental villi and sites of contact with maternal red blood cells
      
To explore the role of calcium in the materno-foetal relationship we decided to study the surface ultrastructure of the human placenta.
      
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  surface structures
Investigation of the Cell Surface Structures of Agrobacteria Involved in Bacterial and Plant Interactions
      
The cell surface structures of agrobacteria were examined by labeling them immunocytochemically with colloidal gold-conjugated antibodies against O-specific lipopolysaccharides, Vir proteins, and cellulase.
      
Agrobacterial cells treated with acetosyringone and brought into contact were found to contain subpolar and polar cell surface structures.
      
Bacteria of the genus Rhodococcus were found to be able to accumulate cesium by means of active transport and nonspecific sorption on the cell surface structures.
      
Principles and mathematic fundamentals of ellipsometric spectral tomography are presented, which may be used in nondestructively monitoring the distribution of physico-chemical properties over the bulk of nonuniform metal-oxide surface structures.
      
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  其他


This article shows the ultrastructural changes of rabbit aortic intima after injection of hypercholesterolemic serum. The chief changes were: protruding of nuclei, widening of gaps between cells, appearance of doable silver staining, line of cell boundaries, separation of adjacent cells from each other and increased number of stigmata and stomata. These changes probably represent contraction of endothelial cells. Considering the work done before, we conclude that contraction of endothelial cells and widening...

This article shows the ultrastructural changes of rabbit aortic intima after injection of hypercholesterolemic serum. The chief changes were: protruding of nuclei, widening of gaps between cells, appearance of doable silver staining, line of cell boundaries, separation of adjacent cells from each other and increased number of stigmata and stomata. These changes probably represent contraction of endothelial cells. Considering the work done before, we conclude that contraction of endothelial cells and widening of gaps between the cells may be closely related to increased permeability of rabbit's sortic intima, which was caused by the injection of hypercholesterolemic serum.

本文得出,静脉注射高脂血清后6小时主动脉内膜表面超微结构出现明显改变,主要表现为细胞核部隆起突出,细胞间隙增宽,边界呈双线,细胞彼此分离,Stigmata和Stomata增多等。这些变化可能是内皮细胞收缩的反映。结合本实验室以前的工作,认为内皮细胞收缩、细胞间隙增宽是静脉注射高脂血清引起内膜通透性增高的结构基础。

In order to elucidate further the cytotoxic effects of silica on macrophages and the cell surface changes,the routine morphology cf the cells stained with acid phosphatase(ACP) was.observed,and the dynamic observations cf the cell surface changes were made at various cultural stages of purified peritoneal mac- rophages from mice by scanning electron microscopy(SEM).The results showed that peritoneal macrophages of the SiO2 group phagccytosed silica particles before 5 minutes,and the cells died,broke down after...

In order to elucidate further the cytotoxic effects of silica on macrophages and the cell surface changes,the routine morphology cf the cells stained with acid phosphatase(ACP) was.observed,and the dynamic observations cf the cell surface changes were made at various cultural stages of purified peritoneal mac- rophages from mice by scanning electron microscopy(SEM).The results showed that peritoneal macrophages of the SiO2 group phagccytosed silica particles before 5 minutes,and the cells died,broke down after 48 hours.The characteristics of the cell outline and a series of cell surface changes between the different stages of cultured and various functional stages were very different in the two groups.The reactionary forms of peritoneal macrophages to silica and the characteristics of the cell surface changes in various stages of phagocytosis were observed.It was suggested that the macrophages phagocytosing silica particles died rapidly and a series of cell surface changes may be due to the cytctoxic effects of silica in the SiO,group.

为探讨二氧化硅(SiO_2)对巨噬细胞的影响,对二组经过提纯的小鼠腹腔巨噬细胞,在扫描电镜下动态地观察了细胞表面超微结构的变化。结果:①随着培养时间及细胞机能状态的不同,两组细胞表现出各自不同的外形及一系列表面超微结构变化的特点。②SiO_2组巨噬细胞在5分钟内开始吞噬粉尘,48小时后变性,失活及崩溃。观察了巨噬细胞对SiO_2尘粒的反应方式及其吞噬过程中不同阶段的形态变化特征。表明:SiO_2组巨噬细胞的迅速死亡及表面超微结构的变化,与被摄入SiO_2粉尘对巨噬细胞的毒性效应有关。

Human esophageal carcinoma cell line,EC109,was examined for the nuclear DNA content by microspectrophotometry,for its ~3H-TdR uptake by radioautography,and for its superficial ultrastructure by scanning electronic microscopy.The DNA content in the interphase nuclei of most EC109 tumor cells were increased,and although the stem line correlated well with the relative DNA content (2C) of G_1 population,the hypotetraploid (3C),hypertetraploid (>4C) and polyploid (>8C)population of tumor cells appeared also.The silver...

Human esophageal carcinoma cell line,EC109,was examined for the nuclear DNA content by microspectrophotometry,for its ~3H-TdR uptake by radioautography,and for its superficial ultrastructure by scanning electronic microscopy.The DNA content in the interphase nuclei of most EC109 tumor cells were increased,and although the stem line correlated well with the relative DNA content (2C) of G_1 population,the hypotetraploid (3C),hypertetraploid (>4C) and polyploid (>8C)population of tumor cells appeared also.The silver grains appeared in many nuclei of tumor cells in S phase within 10 minutes after adding ~3H-TdR into culture medium.A large amount of tumor cells entering cell cycle reflected a hypermalignan- cy.Under the scanning electronic microscope,the superficial ultrastruc- tufts of the EC109 were different in their size,shape and microvilli at different phases of the cell cycle. The investigation showed that the distribution of tumor cells in the various phases of cell cycle can be determined by measuring DNA con- tent,~3H-TdR uptake and superficial ultrastructure,and that the malignan- cy of the tumor could also be determined by these methods.

人食管癌细胞株 EC109以显微分光光度计测定癌细胞核 DNA 含量,以放射自显影测定细胞核摄取~3H-TdR 和扫描电镜检查细胞表面的超微结构.大多数 EC109瘤细胞间期细胞核的 DNA 含量增加,主要干系细胞 DNA 含量为2 C,属 G_1期,也可见低于四倍体(3 C),高于四倍体(>4 C).甚至多倍体(>8 C)的瘤细胞.~3H-TdR 加入培养基10分钟后,许多瘤细胞核有银粒出现(S 期细胞).大量细胞进入细胞周期反映其恶性程度高.扫描电镜可见 EC109在细胞周期不同阶段其细胞大小形状和微绒毛也不同.本文结果说明用细胞核 DAN 含量测定,~3H-TdR 掺入和细胞表面超微结构可测定肿瘤周期.也可用这些方法判定瘤细胞的恶性程度.

 
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