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p蛋白
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  p 32 protein
     Expression of Capripoxvirus P32 Protein
     羊痘病毒P32蛋白的表达
短句来源
     Cloning and expression of capripoxvirus P32 protein gene
     羊痘病毒P32蛋白编码基因的克隆及表达
短句来源
     coli was induced by IPTG. The 35kDa recombinant P32 protein obtained in recombinant Baculovirus and E. coli were detected by SDS-PAGE、Western-blot.
     经 SDS-PAGE、Western- blot鉴定 ,在杆状病毒、大肠杆菌中都得到了 35 k Da重组 P32蛋白
短句来源
     P32 protein is one of the virus structure proteins. It stands out on the virus envelope surface and contains neutralized antigen epitope.
     羊痘病毒P32蛋白是位于羊痘病毒膜表面的主要结构蛋白,含有中和抗原表位;
短句来源
     A 525bp DNA fragment with P32 initiative code in P32 ORF was deleted, so that the recombinant virus mutant would not express P32 protein. The foreign genes could be inserted into the SmaIrestriction enzyme site as well as pdTK. In this study, a LacZ expression cassette was constructed.
     参照构建pdTK转移载体的方法,构建了pdP32转移载体,人为除去了P32基因包括起始密码子在内的525bp的核苷酸序列,完全消除了突变株表达P32蛋白的可能,而且缺失部分不会影响与P32基因相邻的基因结构。
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  “p32蛋白”译为未确定词的双语例句
     Cloning of the gene of capripoxvirus major antigen P32 and construction of its baculovirus expression vector
     羊痘病毒P32蛋白基因的克隆及其杆状病毒表达载体的构建
短句来源
     RT PCR and Western blot were applied to measure the expression of caspase 3 gene and the cleavage of Caspase 3 p32 proteases after a 20 h exposure.
     5 0 μmol/L 6 OH DA处理PC12细胞 2 0h后 ,用RT PCR检测caspase 3表达 ; 用westernblot检测Caspase 3p32蛋白酶原的切割。
短句来源
  相似匹配句对
     32 in the crack region.
     32
短句来源
     32.
     32
短句来源
     Expression of Capripoxvirus P32 Protein
     羊痘病毒P32蛋白的表达
短句来源
     Dlmo Gene Encodes a 32 kDa Protein
     Dlmo基因编码一个32kDa的蛋白
短句来源
     Purification of 32 kD Protein from Maize Pollen and Study on Some of Its Properties
     玉米花粉32kD蛋白的纯化及性质
短句来源
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  p 32 protein
Localization of P32 protein (gC1q-R) in mitochondria and at specific extramitochondrial locations in normal tissues
      
In the present study, we use immunogold electron microscopy to evaluate the subcellular distribution of P32 protein (gC1q-R) in cultured cell lines and in rat tissues embedded in the acrylic resin LR Gold.
      


A detached garlic scape in long storage will eventually give rise to a whorl of freshy aerial cloves at its apex(Text fig.2).This can only be brought about at the expense of the stalk proper,where withering starts from the lower end and extends gradually up- ward until the whole stalk is completely exhausted.The material transfer involved must be mainly concerned with the redistribution and reultilization of cellular contents from the senescing stalk to the growing cloves.The present systematic investigation...

A detached garlic scape in long storage will eventually give rise to a whorl of freshy aerial cloves at its apex(Text fig.2).This can only be brought about at the expense of the stalk proper,where withering starts from the lower end and extends gradually up- ward until the whole stalk is completely exhausted.The material transfer involved must be mainly concerned with the redistribution and reultilization of cellular contents from the senescing stalk to the growing cloves.The present systematic investigation on the whole process is primarily based upon serial microscopic and electron-microscopic examination on conducting channels and withering parenchyma. Our previous investigations on garlic have shown that the exhaustive withdrawl of cellular contents from the senescing tissue is finally accomlished by intercellular move- ment of the partially disassembled protoplasm itself.The present result are essentially in agreement with such a general scheme.Light-and electron-micrographs that show nuclear material and other macro-molecular substances tranversing through the plasmo- desmata are rather common.The high resolving electron-micrographs have enabled us to detect the finer details in intercellular transport as given below: 1.Filamentous and fluffy material,somewhat similar in structure to P-protein in sieve tube,can be found in abundance in senescing parenchyma cells in which the demar- kations between protoplasmic components gradually become indistinct.The filamentous material is in transit through plasmodesmata between parenehyma cells and also between parenchyma and sieve tube(Plate Ⅱ,16,18). 2.Withdrawl of cellular contents from the deteriorating parenehyma may assume the form of vesicular transport through plasmodesmata(Plate Ⅰ 9,10,11).Some of the vesicles are simply filled with vacuolar sap; some fully packed with prefabricated ma- terial of macro-molecular structure;and some actually loaded with disassembled pro- toplasmic fragments. 3.Fully packed vesicles as well as disassembled protoplasmic components(includ- ing disintegrated nucleus,degenerated mitochondrion,etc.) may extrude into the intercel- lular spaces and may invade the vessel cavity (Plate Ⅱ,12,13,20; Plate Ⅲ,21,22,23, 24).The fine strueture of the moving protoplasm in the vessel is quite distinct from that of the residual deposits which may cause plugging in the same cavity(Plate Ⅲ, 25,26).

以贮存蒜苔为材料,研究了在茎苔衰退与顶端珠蒜成长过程中物质的再分配,及其在显微、亚显微结构上的反映。观察到类 P-蛋白丝状物在衰退细胞中的形成及其经胞间连丝在薄壁细胞间、薄壁细胞与韧皮部之间进行运转的多种迹象,提出了大分子物质以集装囊泡的形式进行运输的新论点。进一步发现大分子物质在质外体(导管与细胞间隙)中存在与迁移状态;初步论证了它们与导管堵塞物在质上的差异;指出在特定生理状态下,质外体提供的“自由空间”作为大分子物质主动迁移途径的可能性。

The minor leaf vein of Populus deltoides Bartr. cv. 'Lux' (ex. I-69/55), and P. ×euramericana (Dode) Guinier cv. 'San Martino' (ex. I-72/58) has no A-type and B-type transfer cells, which exist in the herb dicotyledon. But some structures like compound plasmodesma and paramural body that vary morphologically with the development of the leaf can be produced by the companion cells and the phloem parechyma cells of the minor leaf vein. It's shown that their occurrence has something to do with the frequent local...

The minor leaf vein of Populus deltoides Bartr. cv. 'Lux' (ex. I-69/55), and P. ×euramericana (Dode) Guinier cv. 'San Martino' (ex. I-72/58) has no A-type and B-type transfer cells, which exist in the herb dicotyledon. But some structures like compound plasmodesma and paramural body that vary morphologically with the development of the leaf can be produced by the companion cells and the phloem parechyma cells of the minor leaf vein. It's shown that their occurrence has something to do with the frequent local transport. The sieve tube members in the minor leaf vein are differentiated through the formation of the P-protein body, the function of the lysosome in the vacuome and the secretion of the dictyosome. In the two clones of the Aegeiros poplar, i.e., I-69 and I-72 there exist distinctions. It seems that the distinctions are related to the increment. Therefore, they require further research.

Populus delioides Bartr.ev.‘Lux’(ex.I-69/55),和P. ×euramericana(Dode) Guinier ev.‘San Martino’.(ex.I-72/58)无性系后代实生苗叶小脉不具有草本双子叶植物中的A型和B型传递细胞(transfer cell),但小脉伴胞和韧皮薄壁细胞的某些结构-复合胞间连丝和壁旁体(paramural body)的形态是随叶片发育而变化,表明它们的发生与局部频繁的运输有联系。小脉筛管分子的分化是通过 P-蛋白体(P-protein body)的形成和液泡系的溶酶体作用,并伴之以高尔基体的分泌作用。I-69和I-72无性系后代实生苗叶小脉的超微结构有区别,似与生长量有一定的相关性,值得深入研究。

A comparative study on the ultrastructural localization of ATPase activity in the cells of the fifth internode of different species was carried out with cytochemical technique.In the phloem cells of sugarcane stem,ATPase activity was localized on the plasma membrane of sieve elem- ents and companion cells,the nuclei,vescles,full development vacuole of companion cell and P-protein.The ATPase activity showed obvious diffe- rence in the stem phloem cells of varous species.The ATPase activity in the phloem ceils...

A comparative study on the ultrastructural localization of ATPase activity in the cells of the fifth internode of different species was carried out with cytochemical technique.In the phloem cells of sugarcane stem,ATPase activity was localized on the plasma membrane of sieve elem- ents and companion cells,the nuclei,vescles,full development vacuole of companion cell and P-protein.The ATPase activity showed obvious diffe- rence in the stem phloem cells of varous species.The ATPase activity in the phloem ceils of wild species and original cultigens was higher than that of commercial varietes.The results suggested that the phloem ATPase acti- vity in the sugarcane stem be associaed with the transport and accumulation of sugar and the resistibility,and there be P-protein ATPase taking part in the phloem transport of sugarcane stem.

采用酶细胞化学技术对7个甘蔗种和品种进行研究。甘蔗茎韧皮部细胞 ATP 酶活性定位于筛管、伴胞质膜、伴胞核、小囊泡、充分发育的液泡膜和 P—蛋白上。野生种和栽培种茎韧皮部细胞 ATP 酶活性较高,而生产品种则较低。认为甘蔗茎韧皮部 ATP 酶活性与糖分的运输和抗性等有关。茎韧皮运输中,可能有 P—蛋白和 ATP 酶主动参与。

 
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