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   获能精子 的翻译结果: 查询用时:0.019秒
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获能精子
相关语句
  capacitated sperm
     Evaluation of The Human Capacitated Sperm membrane Integrity Using ″Water Test″ Method
     水试验评价人类获能精子膜完整性的研究
短句来源
     After thematured oocytes ( with PBI) were inseminated with in vitro capacitated sperm for 30~70 hrs, 85.% ( 103/128 ) of them fertilized and developed to 2 ~ 8 cell stage or even morula stage.
     将体外成熟的卵母细胞与体外获能精子授精后30~70小时,80.5%(103/128)的卵母细胞受精并可在体外发育到2~8细胞甚至桑椹胚。
短句来源
     Ten oocytes were cocultured for 8 h in mDM with capacitated sperm (1 x lOVmL) and oviductal epithelial cells. The oocytes were then moved into fresh mDM with 20% donor goat serum. Within 24 h after insemination in vitro, 6 of 10 oocytes exposed to sperm appeared to be normal zygotes.
     将获能精子与10枚卵母细胞在受精液(mDM+输卵管上皮细胞)和培养液(mDM+30%供体羊血清)中连续培养(38℃)24h后,有6枚卵受精,抛出第二极体。
短句来源
     It was found that the types of spermatozoa has effects on sperm microinjection system. In the results of injecting one dead sperm into ova plasm. Fertilization rates and eleavage rates were 53.26% and 39.13% for capacitated sperm, 43.21% and 24.69% for uncapacitated sperm, 18.18% and 11.04% for uncapacitated sperm nuclci, respectively.
     卵胞质中注射单个已获能死精子的受精率和发育率分别为53.26%和39.13%,注射单个未获能死精子的受精率和发育率为43.21%和24.69%,而注射单个未获能精子头核的受精率和发育率为18.18%和11.04%。
短句来源
     Although heparin treatments for 45 to 120 min did not differ significantly (P>0 05) in capacitated sperm percentages, sperm m otili ty and membrane integrity decreased significantly when treated with heparin for 120 min.
     其中 ,肝素处理 45~ 12 0min各组的获能精子比例差异不显著 (P >0 0 5) ,处理12 0min组的精子活力和质膜完整率显著低于其它各组。
短句来源
  “获能精子”译为未确定词的双语例句
     ⑤SAF oocytes maturated were fertilization in vitro with capacited sperm and in vitro fertilization rate is 35.7% (51/143).
     ⑤将体外成熟的小腔卵泡卵母细胞与体外获能精子进行受精,其受精率为35.7%(51/143)。
短句来源
     Results:The mean± s proportion of spermatozoa expressing Vn of fertile men was 21.24%±11.70% and 3.64±3.27% for infertile men( P <0.05).
     结果 :生育组与不育组获能精子 Vn表达水平分别为 2 1 .2 4± 1 1 .70 %与3.6 4± 3.2 7% ,不育组明显低于生育组 (P<0 .0 5)。
短句来源
     1.After 3 hours incnabation in Ham F10 medium with 20% FF, the AR rate of sperm non-preincubated,non-capacitated was 5.46% ?
     结果1、20%FF作用于未获能精子3hr,其AR率为5.46%±1.8%。
短句来源
     E_2-BSA also promoted AR and increased [Ca~(2+)]_i in the capacitated human spermatozoa.
     E2诱导获能精子[Ca2+]i升高依赖于胞外Ca2+的内流。
短句来源
     Results E_2 significantly increased the AR rate and the [Ca~(2+)]_i in capacitated human spermatozoa, while it had no effects on the uncapacitated spermatozoa.
     E2-BSA同样可以提高获能精子AR率及[Ca2+]i,其作用与E2相似;
短句来源
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  相似匹配句对
     Advances in Sperm Capacitation
     精子获能的研究进展
短句来源
     IN VITRO CAPACITATION OF RAM SPERM
     羊精子体外获能
短句来源
     A MICROCOMPUTER-BASED IMAGE ANALYSIS SYSTEM FOR HUMAN SPERM QUALITY ANALYSIS
     精子自动分析系统
短句来源
     (5) apoptosis.
     (5 )凋亡精子
短句来源
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  capacitated sperm
After preincubating capacitated sperm with 182 μ/mL of sp18 mAb in the modified TYH IVF medium for 15-20 min, cumulus-oocyte-complexes were introduced.
      
Capacitated sperm were then added and the dishes cultured in a humidified atmosphere at 37°C in 5% CO2+95% air.
      
In fertilized females, cells likely representing capacitated sperm cells are also found in the ovary.
      
Oocytes (n=867) were inseminated with capacitated sperm.
      
A case of human pregnancy after microinjection of capacitated sperm into the perivitelline space
      
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The objective was to obtain in vitro fertilization, early cleavage and normal offspring of rabbit ova. Ovum donors, New Zealand White does, were injected with PMSG or FSH and HCG to induce superovulation for recovery of unfertilized ova. Thirteen hours after treatment with HCG ova were recovered from oviducts and then transferred into a suspension of spermatozoa recovered from the uterine horns of capacitator rabbits which had been mated 18 hr. before by two fertile bucks. Ova were incubated with capacitated...

The objective was to obtain in vitro fertilization, early cleavage and normal offspring of rabbit ova. Ovum donors, New Zealand White does, were injected with PMSG or FSH and HCG to induce superovulation for recovery of unfertilized ova. Thirteen hours after treatment with HCG ova were recovered from oviducts and then transferred into a suspension of spermatozoa recovered from the uterine horns of capacitator rabbits which had been mated 18 hr. before by two fertile bucks. Ova were incubated with capacitated spermatozoa for six hours under a moist 5% CO2, 8% O2 and balance N2 atmosphere at 38℃. Then the ova were removed from the suspension and cultured in 10% serum solution at 38℃ through early cleavage stages. Cleavage rates for in vitro and in vivo (capacitator's ova) fertilized ova were 76.8% (43/56) and 82.3% (14/17), respectively. The embryos were transferred into the oviducts of 5 Chinchilla rabbits that had been injected with HCG at the same time as the donors. One of the recipients died on fourth day after transfer. Fourteen embryos from in vivo fertilized ova transferred into two recipients resulted in two live young. Twenty seven embryos from in vitro fertilized ova transferred into two recipients resulted in five living healthy offspring. The normal development rates of in vivo and in vitro fertilized ova were 14.3% and 18.5%, respectively.

研究获得了我国第一批哺乳动物“试管”后代。 利用供体母兔输卵管中采集的未受精卵,和获能母兔子宫中回收的获能精子进行体外受精。以获能母兔输卵管中采集体内受精卵作对照。结果表明,体外受精和体内受精兔卵的体外发育率分别为76.8%(43/56)和82.3%(14/17)。将体外发育的胚胎分别移植至经同期化处理的青紫兰母兔受体,从体内受精胚胎获得2只仔兔,产仔率为14.3%(2/14),从体外受精胚胎获得5只仔兔,产仔率为18.5%(5/27)。

Effects of two natural proteinase inhibitors (Trichosanthes inhibitor and Ar-rowhead inhibitor) on human sperm penetration into zona-free hamster oocyteswere investigated. The results showed: (1) 1.0×10~(-3) mol/L Trichosanthes in-hibitor added to the spermatozoa containing medium for a period of preincubationintended to induce capacitation caused a large decrease in the spermatozoa penetra-tion rate (P<0.01), while Arrowhead inhibitor of similar concentration did notshow significant effect. (2) The penetration...

Effects of two natural proteinase inhibitors (Trichosanthes inhibitor and Ar-rowhead inhibitor) on human sperm penetration into zona-free hamster oocyteswere investigated. The results showed: (1) 1.0×10~(-3) mol/L Trichosanthes in-hibitor added to the spermatozoa containing medium for a period of preincubationintended to induce capacitation caused a large decrease in the spermatozoa penetra-tion rate (P<0.01), while Arrowhead inhibitor of similar concentration did notshow significant effect. (2) The penetration rate of capacitation spermatozoa intozona-free oocytes pretreated with either inhibitor for 0.5 h was similar to that ofthe untreated control. (3) When either inhibitor (1.0×10~(-3)mol/L or 0.5×10~(-3)mol/L) was present in the capacitation sperm in egg incubation medium, the sper-matozoa penetration rate was significantly decreased (P<0.01). (4) When 1.0×10~(-3) mol/L of either inhibitor was present during both the preincubation for capita-tion and the sperm/egg incubation periods, the ability of spermatozoa to penetratezona-free oocytes was significantly decreased (P<0.01). 1.0×10~(-4) mol/L inhibitorsdid not show up significant effect. (5) The motility of the sperm was not affectedby the inhibitors. Thus it is the enzyme system involved in the penetration ofsperm into oocyte affected by the proteinase inhibitors.

本文研究了慈姑和天花粉两种天然的蛋白水解酶抑制剂对人精子穿透去透明带田鼠卵子的影响。结果显示:(1)在人精子获能期1.0×10~(-3)mol/L天花粉抑制剂可使人精子的穿卵率明显下降(P<0.01),而同样浓度的慈姑抑制剂,作用不明显。(2)去透明带田鼠卵子预先用抑制剂处理0.5h,被获能精子的穿卵率和未用抑制剂处理的卵子相似。(3)在获能后的人精子和去透明带田鼠卵子孵育期,两种抑制剂的浓度为0.5或1.0×10~(-3)mol/L时,精子的穿卵率明显下降。(4)在人精子获能期及人精子/去透明带田鼠卵子孵育期,两种抑制剂的浓度为1.0×10~(-3)mol/L时,均使精子的穿卵率下降(P<0.01),而抑制剂的浓度为1.0×10~(-4)mol/L时,作用不明显。(5)抑制剂不影响人精子的活动能力。这样看来,涉及精子穿透卵子的酶体系受到了蛋白酶抑制剂的影响。

Bovine follicular oocytes collected from butchery were ma-tured and fertilized with in vitro capacitated spermatozoa. After cultivationfor 21, 22, 24, 26, 28 h, the oocytes which reached metaphase Ⅱ were38.89%,74.16%, 77.78%, 86.67% and 85.71% respectively. After 25 h further incubation it did not significantly increase the maturation percentage.Themorphology of oocytes greatly affected their ability to mature in vitro, the oocytes of A, B and C grades were cultured for 24 h, 94.68%, 55.43% and31.25% of oocytes...

Bovine follicular oocytes collected from butchery were ma-tured and fertilized with in vitro capacitated spermatozoa. After cultivationfor 21, 22, 24, 26, 28 h, the oocytes which reached metaphase Ⅱ were38.89%,74.16%, 77.78%, 86.67% and 85.71% respectively. After 25 h further incubation it did not significantly increase the maturation percentage.Themorphology of oocytes greatly affected their ability to mature in vitro, the oocytes of A, B and C grades were cultured for 24 h, 94.68%, 55.43% and31.25% of oocytes reached metaphase Ⅱ respectively. When oocytes of cul-ture delayed for 8h in vitro, the proportion of mature oocytes was muchless than that for 0.75 h or 4h. The time of first sperm penetrating intoooplasm was at 6h after insemination. The sperm head enlarged after 2-3hand developed into early pronucleus stage after another 6h. The time ofthe first cleavage was at 30h after insemination.

本试验主要研究了牛卵泡卵体外成熟、体外受精及其受精过程的发生。从屠宰场收集800枚牛卵泡卵进行体外成熟培养,并用体外获能的精子体外受精。在用m-TCM199培养21、22、24、26、28h后,达到中期Ⅱ的卵母细胞比例分别为33.89%、74.16%、77.78%、86.67%、85.71%;25h后,如果继续培养,成熟的卵母细胞比例不再显著增加。卵母细胞的自身形态显著地影响其成熟效果,A、B、C级卵培养24h后分别有94.60%、55.43%、31.25%的卵母细胞达到中期Ⅱ。卵泡卵在体外滞留8h后,其成熟率(47.11%)明显地低于45min(77.92%)或4h(74.60%)。肝素和钙离子载体A 23187都能诱发牛精子获能,二者在受精效报上差异不显著(P>0.05),并且都有多精入卵现象发生。授精后6h精子进入卵内,精子入卵后2~3h头部膨大,6h后出现早期原核,最早发生卵裂是在授精后30h。

 
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