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前列腺癌     
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  prostate cancer
    Influences of androgen on the growth of human prostate cancer PC-3M model in nude mice and the changes of the androgen receptor levels and protein kinase C activity
    雄激素对人前列腺癌(PC—3M)裸鼠模型生长的影响及其雄激素受体和蛋白激酶C变化特性
短句来源
    Establishment of a lymph node metastatic model with human prostate cancer cell subclone PC-3M-1E8
    利用PC-3M-1E8细胞亚系建立人前列腺癌淋巴道转移模型
短句来源
    Establishment of Animal Bone Metastasis Models of Human Prostate Cancer
    人前列腺癌骨转移动物模型的建立
短句来源
    Results:A120bp cDNA was amplified by RT-PCR from prostate cancer tissue and the sequencing result showed it was the domain of the third extron in ODC gene.
    结果:用RT-PCR方法从人前列腺癌组织中扩增出120bp的cDNA片段,经测序证实为ODC基因第三外显子的片段。
短句来源
    Methods: Human Lc repertorie gene was amplified by RT-PCR from PBMC of prostate cancer patients and was cloned into pComb3X vector containing murine Fd gene to construct the human-mouse hybrid Fab phage library.
    方法:PCR扩增前列腺癌患者外周血淋巴细胞人抗体全套轻链基因,克隆人含鼠源抗γ-sm抗体Fd基因的pComb3X噬粒中,建立人-鼠杂合Fab噬菌体抗体库。
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  prostate carcinoma
    Establishment of Human Prostate Carcinoma Xenograft in Nude Mice and its Radioimmunoimaging
    荷人前列腺癌裸鼠移植瘤模型的建立及其应用研究
短句来源
    Methods: A piece of human prostate carcinoma (PCA) PC 3m was transplanted subcutaneously into 24 male Balb/c nude mice.
    方法 :将人PC 3m前列腺癌块移植于 2 4只Balb/c雄性裸鼠皮下。
短句来源
    Establishment of orthotopic nude mouse model of human prostate carcinoma by surgical implantation
    人类前列腺癌裸鼠原位移植模型的建立
短句来源
    PTEN is inactivated by somatic mutation in increasing variety of malignant tumors both advanced and early stages, such as prostate carcinoma, glioblastoma, hepatocarcinoma, kidney carcinoma and endometrial carcinoma.
    PTEN是一种抑癌基因,PTEN体细胞突变可出现在许多恶性肿瘤的早期和晚期,如前列腺癌、脑胶质瘤、肝癌、肾癌、子宫内膜癌的发生、进展。
短句来源
    It has been reported that expression of PSP94 and PSP57 mRNA is found in prostate carcinoma and benign prostatic hyperplasia(BPH), but PSP57 cDNA is not found in prostate tissue from normal adult. This paper analyzes the expression of PSP mRNA in prostate tissue from five normal Chinese by RT PCR and cDNA sequence determination. The results show that both PSP94 and PSP57 cDNA are found in prostate tissue from these five individuals.
    结果表明,在这5名正常前列腺组织中均同时存在PSP94和PSP57cDNA,且基因序列与文献报道的从前列腺癌和增生前列腺组织中获得的完全一致。
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  prostatic cancer
    Methods The expression of EGFR and PCNA were studied by means of immunohistochemical ABC technique in tissues of 20 cases benign prostatic hyperplasia (BPH) and 36 cases of prostatic cancer (PCa) .
    方法 应用免疫组织化学ABC法,检测36例前列腺癌(PCa),20例前列腺增生(BPH)组织中EGFR、PCNA的表达情况。
短句来源
    PSA is a specific tumor marker of prostatic cancer.
    PSA是前列腺癌的特异肿瘤标志物。
短句来源
    Aim: To construct the single chain variable fragment (ScFv) gene specific for human γseminoprotein, for the genetic construction and expression of a fusion protein of the ScFv and carboxypeptidase A, which could be used in antibodydirected enzyme prodrug therapy for prostatic cancer.
    目的:构建抗人精浆蛋白的单链抗体基因,可为进一步构建、表达抗人精浆蛋白单链抗体/羧肽酶A融合蛋白,用于前列腺癌的抗体导向酶-前体药物疗法奠定基础。
短句来源
    Conclusions The results indicate that HSP70 is required for androgen-insensitive prostate cancer cell to proliferate and survive under normal condition, and HSP70 antisense oligomer can induce apoptosis and inhibit the growth of PC-3m by abrogating its HSP70 expression,moreover,HSP70 might be a new gene modulating the apoptosis of prostatic cancer cells and may be used as a novel target for the gene and drug therapy of prostatic carcinoma.
    HSP70反义寡核苷酸通过抑制HSP70表达而诱导PC 3m凋亡并抑制其生长 ; HSP70可能是一种调控前列腺癌细胞凋亡的新基因 ,可作为基因及药物治疗前列腺癌的新靶向。
短句来源
  “前列腺癌”译为未确定词的双语例句
    Establishment of flutamide insensitive animal model
    氟他胺耐受前列腺癌动物模型的建立
短句来源
    Methods: For 9 male nude mice,Du145(5×10~(6))was injected in tibia by a TB syringe with a 29-gauge needle at a dose of 30 μl per mouse.
    方法:9只6周龄雄性BALB/C裸鼠麻醉后,将前肢及左后肢固定,以1 m l TB针筒-29G针头从胫骨头关节窝,刺入骨髓腔缓缓注入人前列腺癌Du145细胞悬液30μl(约5×106个细胞)至骨髓腔,以建立前列腺癌骨转移模型。
短句来源
    RESULTS: ①Denonvilliers' fascia and the prostatic capsule adhered tightly at the center of the posterior aspect were found in 25 cases (96%, 25/26) of the normal prostate group, and in 44 cases (98%, 44/45) of the radical prostatectomy group respectively.
    结果:①Denonvillier筋膜与前列腺包膜在前列腺后面正中融合者正常组和前列腺癌根治组分别有25例(96%,25/26),44例(98%,44/45)。
短句来源
    ②The lateral pelvic fascia and the prostatic capsule were separated by adipose tissue in 14 cases (54%, 14/26) of the normal prostate group, and in 25 cases (56%, 25/45) of the radical prostatectomy group, and were adhered in 12 cases (46%, 12/26) and 20 cases (44%, 20/45) respectively in above two groups.
    ②14例(54%,14/26)正常组和前列腺癌根治组25例(56%,25/45)标本盆侧筋膜在侧面和前列腺包膜由脂肪组织隔开,12例(46%,12/26)正常组和前列腺癌根治组20例(44%,20/45)结合。
短句来源
    No prostatic capsule could be seen at the anterior aspect in 23 cases (89%, 23/26) of the normal group as well as in 41 cases (91%, 41/45) of the radical prostatectomy group. In 3 cases (11%, 3/26) of the normal group and 4 patients (9%, 4/45) of the radical prostatectomy group, the capsule could be discriminated from the anterior fibromuscular stroma.
    在正常组23例(89%,23/26)和前列腺癌根治组41例(91%,41/45)标本中,在前列腺前面看不到前列腺包膜,在正常组3例(11%,3/26)和前列腺癌根治组4例(9%,4/45)标本中可以分辨出前列腺包膜和前纤维肌肉基质。
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  prostate cancer
The possibility of using AGR2 as a diagnostic marker of prostate cancer is discussed.
      
Calcitriol induces transcription of the placental transforming growth factor β gene in prostate cancer cells via an androgen-ind
      
Calcitriol (1α,25-dihydroxycholecalciferol) suppresses the growth of prostate cancer cells.
      
Growth suppression of hormone-sensitive LNCaP prostate cancer cells by calcitriol is believed to depend on androgens, but the mechanisms of the interactions between the calcitriol-and androgen-dependent signaling pathways is unclear.
      
It was assumed that calcitriol stimulates production of PTGF-β independently of 5α-dihydrotestosterone and that its effect on prostate cancer cell growth is partly mediated by an androgen-independent mechanism.
      
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  prostate carcinoma
Signaling Pathways That Control the Growth and Survival of Prostate Carcinoma Cells in the Absence of Androgens
      
The feasibility of daily CT-based 3D treatment planning is studied in a patients with localized prostate carcinoma using a new patients positioning system.
      
CT is widely used for conformal radiotherapy treatment planning of prostate carcinoma.
      
Comparative Treatment Planning on Localized Prostate Carcinoma
      
Retrovirus-mediated CXCR4-siRNA can inhibit the invasion of prostate carcinoma in vitro
      
更多          
  prostatic cancer
As far as prostatic cancer was concerned, milk was most closely correlated (r=0.711) with its incidence, followed by meat and coffee.
      
Stepwise-multiple-regression analysis identified milk, meat, butter and coffee as significant factors contributing to the incidence of prostatic cancer (R=0.993).
      
Objective: To diagnose prostatic cancer (CaP) with cDNA macroarray.
      
The significance of PSA modified parameters (F/T)/PSAD for diagnosing prostatic cancer in the grey zone of 4~10 ng/ml
      
The objective of this study was to test the hypothesis that prostatic cancer is associated with the changes of zinc (Zn) and cadmium (Cd) concentration.
      
更多          
  prostate adenocarcinoma
Androgen-dependent human prostate adenocarcinoma cell line LNCaP was used to study the effect of androgen deprivation on the cell response to TNF-related cytokines.
      
The results suggested a role of cytokines in the survival of prostate adenocarcinoma cells deprived of androgens in vitro.
      
Inflammation has been proposed to be a precursor of prostate adenocarcinoma.
      
Inflammation has been proposed to be a precursor of prostate adenocarcinoma.
      
Background:Neuroendocrine (NE) differentiation of prostate adenocarcinoma has received increasing attention in recent years as a result of possible implications for prognosis and therapy.
      
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Rb gene is a cancer suppressor gene. Its product, Rb protein has close relation with the regulation of cell transformation, cell cycle and transcription. A lot of carcinomas such as retionblastoma, brest cancer, small cell lung cancer, prostate cancer, bladder cancer, oesteosacoma, soft tissue sarcoma etc. are related to the deletion or inactivation of Rb gene. Stomach cancer is a very common carcinoma of the gastrointestinal system. Researchers have shown that the development of stomach cancer is due to the...

Rb gene is a cancer suppressor gene. Its product, Rb protein has close relation with the regulation of cell transformation, cell cycle and transcription. A lot of carcinomas such as retionblastoma, brest cancer, small cell lung cancer, prostate cancer, bladder cancer, oesteosacoma, soft tissue sarcoma etc. are related to the deletion or inactivation of Rb gene. Stomach cancer is a very common carcinoma of the gastrointestinal system. Researchers have shown that the development of stomach cancer is due to the activation of many oncogenes and the inactivation of antioncogenes. By Southern Blot hybridization, we have tested cancer tissues of nine stomach cancer patients and one cell line BGC-823 with Rb gene probe, finding that the Rb gene of all the tissues and the cell line are abnormal as compared with that of normal human blood tissues. Among the cancer tissues of the nine patients, five have partial deletion of Rb gene, three as well as the cell line have complete deletion of Rb gene. This indicates that the loss of Rb gene is the key link of cancer development. We believe that, with the more sensitive method of Polymerase Chain Reaction (PCR), we can test the fallen cells of stomach hoping that it would be helpful to the early diagnosis of stomach cancer. Meanwhile, this experiment provides target cells of gene therapy research of tumors.

Rb基因是一种抗癌基因,其产物Rb蛋白与细胞转化、细胞周期调节及转录调控有密切关系。许多肿瘤如视网膜母细胞瘤、乳腺癌、小细胞肺癌、前列腺癌、膀胱癌、骨肉瘤、软组织肉瘤等,都与Rb基因的失活或缺失有关。胃癌是消化道常见多发肿瘤,研究表明,其发生发展与多种癌基因的活化、抗癌基因的异常有关。我们用Southern印迹杂交法,以Rb基因为探针,检测了九例胃癌患者的癌组织和一个胃癌细胞株,发现它们与正常人血相比Rb基因全部呈现异常。其中5例发生Rb基因部分缺失,3例及人胃腺癌细胞株BGC-823全部缺失。这表明在胃癌发生过程中Rb基因的丢失是一个重要环节。我们相信用更灵敏的多聚酶链式反应(PCR)技术检测胃脱落细胞,对患癌危险性的早期预报会有很大帮助。同对,本实验也对肿瘤的基因治疗研究提供了靶细胞。

We report a fast efficient method to purify prostate specific antigen(PSA) from human seminal fluid. The procedure only includes two chromatography steps to obtain homogenous PSA, which are Affigel-Blue gel chromatography and gel filtration HPLC. PSA is a specific tumor marker of prostatic cancer.

本文报道了一种从人精液中提纯前列腺特异抗原的方法,本方法仅需Affigel-Blue gel柱和凝胶HPLC二步层析,即可得到均一的PSA纯品。这是一个快速、有效的提纯方法。PSA是前列腺癌的特异肿瘤标志物。

Four hybridoma cell strains secreting monoclonal antibodies(McAbs QW1,QW4,QW7 and QW8) were obtained by immunizing mice with human PSA prepared in our laboratory.Hybridoma cell strains injected into the abdominal cavity of mice produced four McAbs with a titer of 10 ̄6,that of QW8 being the highest. Two-site sandwich enzyme-linked immunosorbent assay(ELISA) has been developed by the binding of the McAb coated on a microplate with biotinylated PcAb. Its sensitivity was 1. 0μg/L. The standard curve,accuracy and...

Four hybridoma cell strains secreting monoclonal antibodies(McAbs QW1,QW4,QW7 and QW8) were obtained by immunizing mice with human PSA prepared in our laboratory.Hybridoma cell strains injected into the abdominal cavity of mice produced four McAbs with a titer of 10 ̄6,that of QW8 being the highest. Two-site sandwich enzyme-linked immunosorbent assay(ELISA) has been developed by the binding of the McAb coated on a microplate with biotinylated PcAb. Its sensitivity was 1. 0μg/L. The standard curve,accuracy and precision of the assay were found to comform to the quality control demands through the evaluation of serum dilution curve ,recovery rate and coefficients of variation. The mean of serum PSA in 10 patients with carcinoma of the prostate was 36. 5±2. 3μg/L ,significantly higher than that of the normal males.

用本室纯化的人前列腺特异抗原免疫BALB/c小鼠,经淋巴细胞杂交瘤技术,建立了4株体外稳定分泌抗人前列腺特异抗原的杂交瘤细胞株,分别命名为QW2、QW4、QW7和QW8。将杂交瘤细胞注入同系小鼠腹腔制备腹水,用ELISA方法测得腹水抗体效价为106,其中QW8的抗体滴度最高。QW8腹水经50%硫酸铵沉淀后包被酶标板,建成双抗体夹心ELISA,标准曲线线性良好,灵敏度为1.0μg/L血清稀释曲线,回收率及批内、批间变异系数测定表明,该方法的准确性和精密度均符合质量控制要求。检测81名50岁以下正常男性血清,PSA均值为1.05±0.73μg/L,10例临床确诊为前列腺癌患者的血清PAS均值为36.5±2.3μg/L明显高于正常值。

 
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