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洛阳土种鸡
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  luoyang local chicken
     Cloning and Prokaryotic Expression of IL-2 Gene of Luoyang Local Chicken in E.coli
     洛阳土种鸡IL-2 cDNA的克隆及原核表达
短句来源
     By reverse transcription-polymerase chain reaction (RT-PCR), a cDNA encoding chicken (interferon-gamma) (chIFN-γ) was cloned from Luoyang local chicken embryo splenocytes induced by (Newcastle) disease virus Ⅰstrain.
     应用反转录 聚合酶链式反应 (RT PCR)技术 ,从鸡新城疫I系疫苗接种的洛阳土种鸡胚脾提取的RNA中扩增到干扰素 γ(IFN γ)基因cDNA。
短句来源
     A cDNA encoding Chicken interleukin-2(IL-2)was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from Luoyang local Chicken embryo spleenocytes . Sequences analysis showed that the target gene was Chicken IL-2 and no termination codon.
     应用RT-PCR技术从新城疫I系疫苗诱导的洛阳土种鸡胚脾淋巴细胞中扩增到全长0.43kb的鸡IL-2基因cDNA,将IL-2cDNA克隆到PMD-T载体,测序结果表明,该基因为不含终止密码子的目的基因。
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  相似匹配句对
     Cloning and Prokaryotic Expression of IL-2 Gene of Luoyang Local Chicken in E.coli
     洛阳IL-2 cDNA的克隆及原核表达
短句来源
     The Chicken
    
短句来源
     E.
     E.
短句来源
     The Effects of Adding Green Forage in Diet on the Production Performanc of Local Chicken
     日粮中添加青绿饲料对生产性能的影响
短句来源
     Each new species is described in Latin and Chinese and is illustrated.
     新是柱状(从)菌Termitomyces cylindricus He sp.nov.
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By reverse transcription-polymerase chain reaction (RT-PCR), a cDNA encoding chicken (interferon-gamma) (chIFN-γ) was cloned from Luoyang local chicken embryo splenocytes induced by (Newcastle) disease virus Ⅰstrain. The result showed that the optimal time at which the mRNA of IFN-γ gene was extracted from spleen cells of chicken embryo is 48 h after the embryo was inoculated by 1∶50 and 1∶100 diluted Newcastle disease virus. Sequence analysis showed that the chIFN-γ cDNA is 492 bp and encodes a protein including...

By reverse transcription-polymerase chain reaction (RT-PCR), a cDNA encoding chicken (interferon-gamma) (chIFN-γ) was cloned from Luoyang local chicken embryo splenocytes induced by (Newcastle) disease virus Ⅰstrain. The result showed that the optimal time at which the mRNA of IFN-γ gene was extracted from spleen cells of chicken embryo is 48 h after the embryo was inoculated by 1∶50 and 1∶100 diluted Newcastle disease virus. Sequence analysis showed that the chIFN-γ cDNA is 492 bp and encodes a protein including 164 amino acids. Compared with the reported nucleotide acid sequence of IFN-γ gene and the corresponding amino acid sequence of IFN-γ, the nucleotide acid sequence of the IFN-γ gene cloned by us and the amino acid sequence deduced from the cloned IFN-γ gene shared 99.7% and 100% homology, respectively. It was concluded from the above-mentioned results that the encoding region of IFN-γ gene is highly conservative.

应用反转录 聚合酶链式反应 (RT PCR)技术 ,从鸡新城疫I系疫苗接种的洛阳土种鸡胚脾提取的RNA中扩增到干扰素 γ(IFN γ)基因cDNA。结果表明 ,以 1∶5 0或 1∶10 0稀释度接种后第 4 8h提取的mRNA扩增效果最佳。序列测定显示 ,所克隆的洛阳土种鸡IFN γ基因cDNA与所报道的鸡IFN γ基因cDNA同源性为 99.7% ,相应的氨基酸序列同源性为 10 0 % ,说明鸡IFN γ基因编码区高度保守。

A cDNA encoding Chicken interleukin-2(IL-2)was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from Luoyang local Chicken embryo spleenocytes . Sequences analysis showed that the target gene was Chicken IL-2 and no termination codon.The IL-2 gene was inserted into the bacterial plasmid PET-28a, resulting in the construction of PET-28a-IL2 prokaryotic expression plasmids. The recombiant plasmid PET-28a-IL2 containing IL-2 gene were identified by restriction enzymes analysis and PCR method...

A cDNA encoding Chicken interleukin-2(IL-2)was amplified by reverse transcription-polymerase chain reaction(RT-PCR)from Luoyang local Chicken embryo spleenocytes . Sequences analysis showed that the target gene was Chicken IL-2 and no termination codon.The IL-2 gene was inserted into the bacterial plasmid PET-28a, resulting in the construction of PET-28a-IL2 prokaryotic expression plasmids. The recombiant plasmid PET-28a-IL2 containing IL-2 gene were identified by restriction enzymes analysis and PCR method and sequenced to confirm its rightness. The recombinant fusion protein was highly expression in E. coli BL21(DE_(3))four hours later induced by IPTG and the molecular weight is about 14 ku. These provide a basis for the study on the biological property of chicken IL-2.

应用RT-PCR技术从新城疫I系疫苗诱导的洛阳土种鸡胚脾淋巴细胞中扩增到全长0.43kb的鸡IL-2基因cDNA,将IL-2cDNA克隆到PMD-T载体,测序结果表明,该基因为不含终止密码子的目的基因。将该目的基因克隆到原核表达载体PET-28a,获得的重组质粒PET-28a-IL2经酶切、PCR及序列测定,表明IL-2基因插入的位点、大小与读码框均正确。PET-28a-IL2在大肠杆菌BL21(DE3)中经IPTG诱导,表达产物经SDS-PAGE电泳分析,在14ku处出现一条约占总蛋白21%的蛋白带,表明所克隆的IL-2基因在大肠杆菌中得到良好的表达,为进一步研究IL-2的生物学特性奠定了基础。

 
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