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直接pcr
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  direct pcr
     Comparing the sensitivity of these 2 detecting methods, about 1×10~4cfu/ml of the bacterial suspension were detected by direct PCR and below 1×10~2cfu/ml only can be detected by immunocapture PCR method.
     对两种检测方法的灵敏度比较发现:在配制的浓度梯度菌悬液检测实验中,直接PCR技术能检测到1×10~4cfu/ml左右的悬浮液,免疫捕捉PCR技术能检测到50-100cfu/ml左右悬浮液;
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     Comparing the sensitivity of the 2 detection methods, about 1×10~5cfu/ml of the bacterial suspension of Cmmcould be detected by direct PCR while about lxl06cfu/ml were detected by immunocapture PCR.
     对两种检测方法的灵敏度比较发现:配制的浓度梯度菌悬液检测实验中,直接PCR技术能检测到1×10~5cfu/ml左右的悬浮液,免疫捕捉PCR技术能检测到1×10~6cfu/ml左右悬浮液。
短句来源
     Results: (1)The sensitivity of the new assay,RT-nested PCR,on the CT gene hypermethylation is 1/200 thousand,and it is 200 times than DNA direct PCR method.
     结果 :( 1)所建立的 RT-nested PCR法检测 CT基因异常甲基化 ,其灵敏度为 1/ 2 0万 ,比 DNA直接 PCR法高 2 0 0倍 ;
短句来源
     The minimum detection concentration of the IC-PCR and direct PCR method was about 50~102 cfu/mL and 104 cfu/mL, respectively. The sensitivity of the former was 100 times higher than that of the later.
     免疫捕捉PCR检测果斑病菌的灵敏度为50 ̄102cfu/mL,而直接PCR则为104cfu/mL,两者的灵敏度相差100倍左右。
短句来源
     To establish a direct PCR method for the detection of Trypanosoma evansi from blood samples,primers were designed to amplify the DNA fragment of T. evansi kDNA under optimizing conditions. Ampdirect was applied to extract kinetoplast DNA from blood samples spotted on filter paper.
     为建立伊氏锥虫动基体DNA(KinetoplastDNA,kDNA)的PCR扩增技术,血液直接PCR法检测伊氏锥虫的感染,本文以伊氏锥虫kDNA片段为靶扩增DNA设计引物,确定最适PCR反应条件,建立kDNA片段的PCR扩增技术,应用直接扩增缓冲液(Ampdirect)从感染小鼠的滤纸血标本直接PCR检测伊氏锥虫。
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  “直接pcr”译为未确定词的双语例句
     Methods: Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) and Polymerase chain reaction-temporal temperature gradient gel electrophoresis(PCR-TTGE) were carried out to screen the fragment deletion of mtDNA 4977 and the mutations of tRNA ~(Leu(UUR))A 3243G and mtDNA T14577C.
     方法:采用直接PCR、PCR-限制性片段长度多态性(restriction fragm ent length polymorph ism,RFLP)、PCR-时相温度梯度凝胶电泳(temporal temperature grad ient gel electrophoresis,TTGE)法筛查m tDNA 4977片段缺失、线粒体tRNALeu(UUR)A3243G突变和m tDNA T14577C突变。
短句来源
     A Simple Method for Extracting DNA from Polyacrylamide Gels and Amplifying DNA Directly
     从聚丙烯酰胺凝胶中回收DNA片段直接PCR的简易方法
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     In the meantime, directPCR amplification of blood and amniotic fluid was completed. The fragment was shown from 4 μl to 0.5 μl of blood and from 2 ml to 0.5 ml of amniotic fluid.
     同时进行了全血和羊水的直接PCR,在4μl至0.5μl血时可出现扩增带,羊水在2ml至0.5ml时也出现扩增带。
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     Objectives: 1. To learn the distribution frequencies of ACE, AGT, genotypes in Chinese and to find the relationships between polymorphisms of ACE, AGT ang AT_1R gene with CAD and Ml and to study the interactions of themselves.
     在CAD组,82例确诊心肌梗塞的忠者构成心梗组,余110例为非心梗组。 分别采用直接PCR和PCR-RFLP技术检测ACE基因I/D多态性、AGT基因M235T多态性和AT_1R基因A1166C多态性。
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     A PCR method was developed for the rapid detection of IBV.Direct and nested PCR for amplifying purified N gene showed that two products were 0.7kb and 0.5kb,which were identical to initial designed, while the PCR products of controlled NDV and IBDV were negative.
     本试验建立了一种快速检测鸡传染性支气管炎病毒的通用性PCR方法。 通过对纯化后IBV核蛋白基因进行的直接PCR及套式PCR扩增表明 ,两次PCR产物的大小为 0 .7kb和 0 .5kb ,与实际设计相符。
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  相似匹配句对
     Direct Detection of Mitochondria by Polymerase Chain Reaction
     线粒体直接PCR扩增法
短句来源
     The Studies on Direct PCR using Maize Pollen Grain Suspensions
     玉米花粉粒直接PCR技术研究
短句来源
     Electronic PCR
     电子PCR
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     kill directly the tumor cells;
     直接杀伤;
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     Direct Cloning of Crithidia
     短膜虫的直接克隆
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  direct pcr
Under these conditions, yeast and fungal cell envelopes remain unbroken and retain the original DNA and RNA that could be used for direct PCR amplification.
      
In the direct PCR with the thermostat chip, the whole process only involves automatic thermal programs.
      
SSR loci were successfully amplified by direct PCR from mycelia of P.
      
The aim of this study was to develop a simple method using universal primers for species identification based on direct PCR sequencing.
      
In this study, we employed direct PCR, cloning, and sequencing of narG gene fragments to determine the diversity of nitrate-reducing bacteria occurring in soil and in the maize rhizosphere.
      
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Freezing the single hair bulb in the liquid nitrogen,heating to 94℃ f0r three times and directly using in PCR, we successfully typed the variable number of tandem repeats(VNTR) in the intron 40of the von Willebrand factor(vWF) gene. 62 hair bulbs out of 68 freezed hair samples(91. 2% ) weresuccessfully amplifled and typed. This methed was very simple,sensitive and efficient.

首次用液氮冷冻单根毛发根,再经三次94℃高温处理,直接PCR扩增,从68根毛发根中,成功地检测了62根毛发根的人类vWF基因40内含子VNTR,检出率达91.2%。本方法简便、快速、实用,在法医学检验及群体遗传学研究中有重要应用价值。

Objective: To establish the method for prenatal sex diagnosis of the fetus carrying sexlinked genetic disorder. Method: Human SRY gene was amplified by polymerase chain reaction. A 422 bp male specific fragment was obtained. Results: The fragment was identified in 10 men, but unidentified in 10 women. The diagnostic accordance rate of 20 amniotic fluid samples was 100%. 22 of 47 chorionic villi samples were positive. The rate of positive/negative (22/25) was nearly the sex rate of newborn babies. In the meantime,...

Objective: To establish the method for prenatal sex diagnosis of the fetus carrying sexlinked genetic disorder. Method: Human SRY gene was amplified by polymerase chain reaction. A 422 bp male specific fragment was obtained. Results: The fragment was identified in 10 men, but unidentified in 10 women. The diagnostic accordance rate of 20 amniotic fluid samples was 100%. 22 of 47 chorionic villi samples were positive. The rate of positive/negative (22/25) was nearly the sex rate of newborn babies. In the meantime, directPCR amplification of blood and amniotic fluid was completed. The fragment was shown from 4 μl to 0.5 μl of blood and from 2 ml to 0.5 ml of amniotic fluid. Conclusion: The results show that fetal sex determination by PCR will be suitable for clinical prenatal diagnosis of sexlinked genetic disorders.

目的:建立一种对性连锁遗传病胎儿进行产前性别诊断的方法。方法:采用聚合酶链式反应(PCR)扩增男性特异的SRY基因,扩增片段大小为422bp。结果:对不同标本的扩增表明,10例男性标本中均出现该特异扩增片段,而女性无此片段;20例羊水标本的扩增结果与其新生儿实际性别完全一致;47例绒毛标本中22例阳性,阴性/阳性之比为1∶1.136,接近实际的胎儿性别之比。同时进行了全血和羊水的直接PCR,在4μl至0.5μl血时可出现扩增带,羊水在2ml至0.5ml时也出现扩增带。结论:PCR特异扩增SRY基因片段,在临床上可用于性连锁遗传病的产前性别诊断

Objective To establish a simple technique with which the gradually shortened DNA clones can be selected rapidly and accurately so that the sequencing and identification of the full length cDNA or longer DNA fragments can be performed as quick as possible.Methods Primers were designed according to the sequence flanking the multiple cloning site of pGEM vector and were used to amplify the inserted fragments in a series of deletion sub clones originated from a clone.with longer DNA insert fragment.These sub...

Objective To establish a simple technique with which the gradually shortened DNA clones can be selected rapidly and accurately so that the sequencing and identification of the full length cDNA or longer DNA fragments can be performed as quick as possible.Methods Primers were designed according to the sequence flanking the multiple cloning site of pGEM vector and were used to amplify the inserted fragments in a series of deletion sub clones originated from a clone.with longer DNA insert fragment.These sub clones which contain the gradually shortened fragments can be selected directly.This method was compared with the routine method in which the restriction endonuclease was used to digest DNA samples.Results A series of deletion sub clones were identified accurately with this new method and the cycle sequencing could be performed on these PCR products as well.This method was characteristic of more accurate,more simple and less time consuming,compared with the routine method.Conclusion The method presented is very effective for rapid selection of the gradually shortened inserted fragments constructed in pGEM vector.It can greatly save time and materials,and the strategy in the report could also fit for selecting the clones containing gradually shortened fragments in other plasmid vectors.

目的为了建立一种快速、简便而准确地筛选递次截短的DNA克隆的技术,加速对基因全长cDNA或DNA大片段的测序和鉴定。方法在载体多克隆位点两侧设计引物,直接PCR扩增插入片段,以此为基础选择系列缺失亚克隆,并与常规酶切法作比较。结果根据PCR产物大小排序,准确地鉴定出系列缺失亚克隆,并可将PCR产物直接用于PCR循环测序,比酶切法分辨率高,操作步骤简化,筛选时间短。结果所建立的PCR快速筛选法不仅是一种行之有效的鉴定方法,而且可以显著地节省时间和实验材料。该方法也可适用于其它各种质粒载体。

 
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