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igf-i基因
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  igf-i gene
     A Study on cDNA Clone of GDF8 gene and the mRNA Expression of GDF8 gene and IGF-I gene in Blue fox
     蓝狐GDF8基因cDNA克隆及GDF8基因与IGF-I基因表达研究
短句来源
     Studies on Molecular Genetic Polymorphisms of the IGF-I Gene and MyoG Gene in Pigs
     猪IGF-I基因及MyoG基因分子遗传多态性的研究
短句来源
     Study on Polymorphism of IGF-I Gene on Chickens by PCR-SSCP and Relationship Analysis with Carcass Traits
     鸡IGF-I基因PCR-SSCP分析及其与肉用性状关系的研究
短句来源
     Gene and genotype frequencies were calculated for the polymorphic loci in IGF-I gene and MyoG gene.
     对IGF-I基因和MyoG基因上所检测到的多态性片段分别做了基因频率和基因型频率统计。
短句来源
     CEA and AFP Secretion of Human Hepatoma Cells Transfected by Antisense IGF-I gene
     反义IGF-I基因转染人肝癌细胞分泌CEA和AFP水平的研究
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  igf i gene
     STUDY OF ANTISENSE IGF I GENE AGAINST MICE B16 MEL ANOMA
     反义IGF-I基因转染抗小鼠B16黑色素瘤的研究
短句来源
     The total RNA was extracted from liver of finishing pig(Duroc×Yorkshire×Landrace), and acted as template to synthesize the first strand of IGF I gene cDNA using P1(5′ CTACATTCTGTAGTTCTTGTTTCC 3′) as the primer. Then, the cDNA was amplified by PCR with P1 and P2(5′ ATGGCCCTGTGCTTGCTCTCCTT 3′) primer. A DNA fragment with the length of 360 bp was amplified and cloned into the pGEM T vector.
     采用 TRIzol法从杜大长杂交猪的肝脏中提取总 RNA ,将其纯化后作为 PCR扩增模板 ,以设计的 P1( 5′- CTACA TTCTGTAGTTCTTGTTTCC- 3′)为引物合成 IGF- I基因 c DN A的第 1链 ,再以 P1和 P2 ( 5′-ATGGCCCTGTGCTTGCTCTCCTT- 3′)为引物扩增到大小约为 36 0 bp的产物 ,并将其克隆至 p GEM- T载体上。
短句来源
     It shares 100% homology with the porcine IGF I gene reported by Muller M and Brem G.
     与 Gen Bank中的序列比较 ,本研究所得序列与 Muller M和 Brem G所报道的猪 IGF- I基因编码区序列完全一致。
短句来源
  “igf-i基因”译为未确定词的双语例句
     IGF-I exon3, IGF-I exon4, MyoG exon3, MyoG5’-1 and MyoG5’-3 appeared polymorphic.
     检测结果发现,在IGF-I 基因的外显子3 和外显子4,以及MyoG 基因外显子3、MyoG5'-1 和MyoG5'-3 共5 个座位上存在多态性。
短句来源
     Construction of eukaryotic expression recombinant plasmid pIRES2-EGFP-IGF-Ⅰ for the human IGF-Ⅰ gene
     人IGF-I基因真核表达质粒pIRES2-EGFP-IGF-I的构建
短句来源
     (2) The polymorphisms of PCR-SSCP and PCR-RFLP were not observed in the 5’flankingregion, 5th exon, and 5th intron of bovine insulin-like growth hormone factor (IGF-I).
     3. 牛 IGF-I 基因第 2外显子、5’端、第 5外显子、第 5内含子座位多态以及与牛生长发育性状的关系分析(1)首次在牛 IGF-I 基因第 2 外显子上发现 PCR-SSCP 多态,多态位点有一对等位基因 A、B,该多态位点的产生是由于 3620bp 处碱基 A→G 的突变;
短句来源
     Methods A suspension of murine colon 26 adenocarcinoma cells was inoculated subcutaneously into the control and liver-specific IGF-I deficient(LID) mice,respectively,n=30 in each group.
     方法将小鼠结肠腺癌26细胞悬液接种于肝脏特异性IGF-I基因缺失(LID)组鼠和对照组鼠皮下,各30只接受了肿瘤细胞接种。
短句来源
     Statistical analysis(ANOVA)showed that there was certain correlation between the bone mineral density(BMD)of humerus and IGF-I genotype,BMD and bone mineral content(BMC)of humerus of PP genotype were obviously higher than those of pp genotype(P<0.05).
     方差分析显示,肱骨的骨密度与IGF-I基因多态性相关,PP基因型组的骨密度和骨矿含量均显著大于pp基因型(P<0.05),股骨、胫骨基因型间无显著差异。
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  igf-i gene
The present experiment examines IGF-I gene transfer in vitro.
      
These observations argue that vector-mediated IGF-I gene transfer induces secretion of active IGF-I that acts through direct effects on spinal cord MNs.
      
Therefore, we have developed a mouse model with a liver-specific inducible deletion of the IGF-I gene (LI-IGF-I-/- mouse).
      
When the cells were transfected with a human IGF-I gene, significant amounts of the IGF-I protein were secreted.
      
A cDNA probe corresponding to mRNA encoding human somatomedin-C/insulin-like growth factor I (IGF-I) was used for the chromosomal assignment of the IGF-I gene.
      
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  igf i gene
GH stimulates IGF I gene expression in collecting duct.
      


This article has introduced the gene and it's expression of IGF—I,the production of IGF— I in the body and how it is regulated by development,various hormones and nutritions as well as the nature of these regulating pathways.

本文介绍了 IGF—I 的基因及其表达,体内 IGF—I 产生如何受发育、各种激素和营养的调节,以及这些调节途径的性质。

In this research, we apply the antisense strategy to study the function of IGF- I in human hepatoma lines(7402) by Lipofectin mediated gene transfer. The effect of antisense IGF-I on the expression of MHC and the sensitivity of LAK cytotoxicity of tumor cell were studied. The results showed that human hepotoma lines(7402) were transfected with an amplifiable antisense IGF- I gene. The expression antisense IGF-I RNA in the transfected cells after hygromycin resistant selection was confirmed by northern blot analysis....

In this research, we apply the antisense strategy to study the function of IGF- I in human hepatoma lines(7402) by Lipofectin mediated gene transfer. The effect of antisense IGF-I on the expression of MHC and the sensitivity of LAK cytotoxicity of tumor cell were studied. The results showed that human hepotoma lines(7402) were transfected with an amplifiable antisense IGF- I gene. The expression antisense IGF-I RNA in the transfected cells after hygromycin resistant selection was confirmed by northern blot analysis. The transfectants expressed higher level of MHC class I and class II molecules. The susceptibility of the transfected cell to LAK cytotoxicity was greater than that of 7402 parent cells(P<0.05). It suggests that transfecting cells can enhance the expression of MHC class I and class II molecules and suceptibility of 7402 cells to the cytotoxicity of LAK cells.

本研究利用基因治疗方法中的反义技术,通过脂质体包裹反义胰岛素样生长因子-I(IGF-I)基因导入人7402肝癌细胞株,研究肿瘤细胞表面MHC分子的表达和其对LAK细胞杀伤的敏感性。结果表明,反义IGF-I基因转染的人7402肝癌细胞,经潮霉素筛选后,Northern Blot分析显示反义IGF-I RNA表达阳性,MHC Ⅰ类和Ⅱ类抗原的表达水平高于亲本7402细胞。LAK细胞对反义IGF-I基因转染的人7402肝癌细胞的杀伤活性明显高于亲本7402细胞组(P<0.05)。说明反义IGF-I基因转移至该肝癌细胞,可提高肿瘤的MHC Ⅰ类和Ⅱ类分子的表达,显示肿瘤细胞的免疫原性增强,并可提高LAK细胞对其杀伤的敏感性。

The total RNA was extracted from liver of finishing pig(Duroc×Yorkshire×Landrace), and acted as template to synthesize the first strand of IGF I gene cDNA using P1(5′ CTACATTCTGTAGTTCTTGTTTCC 3′) as the primer. Then, the cDNA was amplified by PCR with P1 and P2(5′ ATGGCCCTGTGCTTGCTCTCCTT 3′) primer. A DNA fragment with the length of 360 bp was amplified and cloned into the pGEM T vector. Through transferring, screening, enzymolysis and sequencing, it was demonstrated that this fragment was IGF I gene...

The total RNA was extracted from liver of finishing pig(Duroc×Yorkshire×Landrace), and acted as template to synthesize the first strand of IGF I gene cDNA using P1(5′ CTACATTCTGTAGTTCTTGTTTCC 3′) as the primer. Then, the cDNA was amplified by PCR with P1 and P2(5′ ATGGCCCTGTGCTTGCTCTCCTT 3′) primer. A DNA fragment with the length of 360 bp was amplified and cloned into the pGEM T vector. Through transferring, screening, enzymolysis and sequencing, it was demonstrated that this fragment was IGF I gene cDNA. It has 360 nts in length and contains 1 ORF, which encode a polypeptide containing 118 amino acids. It shares 100% homology with the porcine IGF I gene reported by Muller M and Brem G.

采用 TRIzol法从杜大长杂交猪的肝脏中提取总 RNA ,将其纯化后作为 PCR扩增模板 ,以设计的 P1( 5′- CTACA TTCTGTAGTTCTTGTTTCC- 3′)为引物合成 IGF- I基因 c DN A的第 1链 ,再以 P1和 P2 ( 5′-ATGGCCCTGTGCTTGCTCTCCTT- 3′)为引物扩增到大小约为 36 0 bp的产物 ,并将其克隆至 p GEM- T载体上。经筛选、酶切、序列分析 ,表明该片段为 IGF- I基因的 c DNA克隆 ,它由 36 0个核苷酸组成 ,包含一个完整的开读框 ( ORF)。该开读框编码一个由 118个氨基酸组成的多肽 ,即 IGF- I。与 Gen Bank中的序列比较 ,本研究所得序列与 Muller M和 Brem G所报道的猪 IGF- I基因编码区序列完全一致。

 
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