助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   植酸酶基因 的翻译结果: 查询用时:0.81秒
图标索引 在分类学科中查询
所有学科
生物学
农作物
畜牧与动物医学
更多类别查询

图标索引 历史查询
 

植酸酶基因
相关语句
  phytase gene
     Cloning and Expression of appA Phytase Gene and Nattokinase Gene
     appA植酸酶基因与纳豆激酶基因的克隆与表达研究
短句来源
     P1, P2 were designed according to the consensus sequence of phytase gene.
     根据植酸酶基因的保守序列设计引物P1、P2,从云芝中分离克隆植酸酶基因的片段.
短句来源
     Over-expression of Aspergillus niger N14 Phytase Gene in Pichia pastoris
     黑曲霉N14植酸酶基因在巴斯德毕赤酵母中的高效表达
短句来源
     Cloning of Phytase Gene from Coriolus versicolor by TAIL-PCR and RT-PCR
     利用TAIL-PCR和RT-PCR技术克隆云芝植酸酶基因
短句来源
     Construction of Bacterial Phytase Gene in pEGFP-N_3 Vectors and Its Expression in COS7 Cells
     pEGFP-植酸酶基因质粒重组构建及其在COS7细胞中表达分析
短句来源
更多       
  phytase genes
     A pair of primers were designed according to the published sequences of the phytase genes(GI:4815609 and GI:22901877 in GenBank). The target fragment from the genome DNA of Asperillus oryae Cohn(No.ACCC30155) by PCR was amplified.
     根据已发表的植酸酶基因phyA序列(GI:4815609和GI:22901877)设计引物,以米曲霉(AsperillusoryaeCohn,编号:ACCC30155)的总DNA为模板,利用PCR扩增得到目的片段。
短句来源
     A lot of phytase genes have been successfully cloned and expressed by researchers, which generated industrially in high yield of phytate.
     国内外科技工作者对植酸酶基因克隆及其表达的研究已有许多成功的报道,大幅度提高了植酸酶的产量,并且实现了工业化的生产。
短句来源
     In the field of industry, phytase genes integrated into engineering microorganisms were from Aspergillus niger most times for that Aspergillus niger is able to synthesis phytase possess the highest avtivity. But phytase from Aspergillus niger can not endure high temperature and the activity decreases drastically during drying and particle-formation.
     一般认为黑曲霉(Aspergillus niger)产生的植酸酶活性最强,因此工业化生产的工程菌中的植酸酶基因大都来自于黑曲霉,但是这种来源于黑曲霉的植酸酶不能耐受较高的温度,在干燥和制粒加工过程中酶活大量丧失。
短句来源
     The phytase gene region was cloned by PCR amplification from Flammulina velutipes genomic DNA with a pair of specific primers designed according to the conserved sequence of phytase genes in the GenBank.
     根据GenBank中植酸酶基因的保守区设计并合成一对特异性引物,以金针菇菌丝的总DNA为模板,通过PCR扩增,获得了一条长约790bp的片段。
短句来源
     Phytase gene of Bacillus is very different from the phytase genes known before, so it' s a kind of new phytase.
     该基因与已知植酸酶基因的结构有所 不同,是一种新的植酸酶。
短句来源
  “植酸酶基因”译为未确定词的双语例句
     Overexpression of Phytase (phyA) Gene from Aspergillus niger NRRL 3135 in Pichia pastoris GS115
     黑曲霉NRRL3135菌株植酸酶基因在毕赤酵母GS-115系统中的表达
短句来源
     Cloning and Sequence Analysis of the PhyB Gene of Aspergillus niger WP1
     植酸酶基因PhyB_1的克隆及序列分析
短句来源
     The phytase(phyA) gene from Aspergillus niger NRRL 3135 was inserted into the chromosome DNA of Pichia pastoris GS115 and was expressed under the induction.
     将黑曲霉(Aspergillus niger)NRRL3135中的植酸酶基因转入毕赤酵母GS-115中,并整合到染色体DNA上,然后进行诱导表达。
短句来源
     3)transgenic pigs containing genes coding for phytase.
     3)通过基因编码植酸酶来提高动物的代谢潜能(转植酸酶基因猪)。
短句来源
     Overexpression of Artificial Synthetic Gene ofAspergillus niger NRRL3135 Phytase in Pichia pastoris
     人工合成的黑曲霉NRRL3135菌株植酸酶基因在毕赤酵母系统中的高效表达
短句来源
更多       
查询“植酸酶基因”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  phytase gene
Isolation and characterization of a novel phytase gene (Sphy1) from soybean (Glycine max (L.) Merr.)
      
A novel phytase gene Sphy1 was isolated based on screening a cDNA library which was constructed from germinated soybean (Glycine max (L.) Merr.
      
A novel β-propeller phytase gene (phyS), cloned from Shewanella oneidensis MR-1, was found to encode a protein with two beta-propeller phytase domains.
      
Positive identification of a lambda gt11 clone containing a region of fungal phytase gene by immunoprobe and sequence verificati
      
niger lambda gt11 expression library and the use of amino acid sequencing to identify a clone containing part of the fungal phytase gene.
      
更多          
  phytase genes
Phylogenetic analysis indicated that Sphy1 had high similarities with the phytase genes from Medicago truncatula and rice, and acid phosphatase genes from M.
      
To study the natural variability in amino acid sequence and its impact on the catalytic properties of the enzyme, we cloned and overexpressed the phytase genes and proteins from six new purported A.
      
Transgenic expression of phytase genes of plant origin has great potential for improving plant phosphorus acquisition and for phytoremediation.
      
Structure of two maize phytase genes and their spatio-temporal expression during seedling development
      
Availability of inositol phosphates in soil is a major factor that limits the effectiveness of expressing fungal phytase genes in plants as a means to improve P-nutrition.
      


Aspergillus niger 963 secreting phytase is isolated from soil . It is cultivated by a shaking method and phytase is pro - duced 165 U/mL . The phytase shows following properties . a ) Optimal pH values of the phytase for its activity are 1. 8 and 5 . 7 . b) Optimal temperature of the phytase for its activity is about 55 ℃ . The gene phy A2 encoding the phytase is cloned from A. niger 963 by polymerase chain reaction( PCR) . Nucleotide sequence analysis of phy A2 revealed that the presence of an open reading...

Aspergillus niger 963 secreting phytase is isolated from soil . It is cultivated by a shaking method and phytase is pro - duced 165 U/mL . The phytase shows following properties . a ) Optimal pH values of the phytase for its activity are 1. 8 and 5 . 7 . b) Optimal temperature of the phytase for its activity is about 55 ℃ . The gene phy A2 encoding the phytase is cloned from A. niger 963 by polymerase chain reaction( PCR) . Nucleotide sequence analysis of phy A2 revealed that the presence of an open reading frame cod- ing for 467 amino acids and interrupted once by an intron of 102 bp in the 5' put of gene . The start codon is followed by a sequence coding for a putative signal peptide ( 19 amino acids in length ) . There are nine potential N - glycosylation sites in phytase . Compari- son with phytase gene phyA from A . niger ( ficuum) var . awamori shows 91 . 8% homology in nucleotides and 91. 6% in amino acids residues .

从土壤中筛选到产植酸酶的黑曲霉菌株Aspergillusniger963,对其所产植酸酶的生理生化性质研究表明其具有适合于在饲料中使用的优良性质。它的酶活性最适温度为55℃,最适pH值分别为pH1.8和5.7,并且在pH1.8-5.7的范围内,植酸酶均能维持较高的酶活性。摇瓶发酵实验表明,植酸酶的表达量为165U/mL发酵液。通过PCR的方法克隆了植酸酶基因扯phyA2,DNA全序列分析表明其结构基因全长1506个核苷酸(编码467个氨基酸),5’端有一编码19个氨基酸的信号肽序列,其中包含一段长102个核苷酸的内含子。此序列与曾报道的源于Aspergillusnigervar.awamori的植酸酶基因phyA有一定的差异,其DNA序列同源性为91.8%,所编码的蛋日同源性为91.6%,相应的酶学性质也有所不同。

Benzyl chloride were used for extrcting fungal DNA.Under the condition of pH9.0 and incubated at 50?℃ for an hour after benzyl chloride was mixed enough with fungal cells, Genomic DNAs of Rhizopus chinensis Rc1 and Rhizopus arrhizus Ra1 were extracted and isolated from their integrated cells and from the mycelial powder of Aspergillus ficuum AS3.324 and Trichosporon ficuum Tf1 grinded in liquid nitrogen with high yield and little contamination of protein or other organic components.Molicular...

Benzyl chloride were used for extrcting fungal DNA.Under the condition of pH9.0 and incubated at 50?℃ for an hour after benzyl chloride was mixed enough with fungal cells, Genomic DNAs of Rhizopus chinensis Rc1 and Rhizopus arrhizus Ra1 were extracted and isolated from their integrated cells and from the mycelial powder of Aspergillus ficuum AS3.324 and Trichosporon ficuum Tf1 grinded in liquid nitrogen with high yield and little contamination of protein or other organic components.Molicular sizes of all the isolated DNAs were higher than 23kb.After PCR was operated by using the isolated genomic DNAs of AS3.324 as template and using a double of specifical primers, a 1.4 kb segment of phyA was obtained.

研究了利用氯化苄提取丝状真菌的基因组DNA。在 pH9.0条件下 ,使氯化苄与菌体细胞充分混合后于 50℃保温 1h ,可以从中华根霉 (Rhizopuschinensis) Rc0 1和少根根霉 (Rhizopusarrhizus)Ra0 1的完整细胞及经液氮研磨的无花果曲霉 (Aspergillusficuum ) AS3.32 4和无花果丝孢酵母 (Trichosporonficuum) Tf0 1的菌粉中提取基因组DNA。所提DNA收获量大 ,蛋白质污染少 ,分子量均在 2 3kb以上 ,一级结构完整。以AS3.32 4基因组DNA为模板在特定引物下 ,可通过PCR扩增出 1 .4kb的植酸酶基因 ( phyA) 。

Using PCR method,We have amplified Aspergillus.ficuumAS3.324 phyA gene from its clone vector, PUC18/phyAI.Then, through restrition digest and T 4 DNA polymerase ligation,we have cloned it to plant medium vector pBI121 and obtained pBI121/phyAⅡ particles with AS3.324 phyA contents.The next step is introducing pBI121/phyAⅡ into Agrobacterium tumefacien through freeze thaw method. Thus, the correct expressing vector—LBA4404/pBI121/phyA is achieved.Its reliability has been affirmed by antiboitic screening and...

Using PCR method,We have amplified Aspergillus.ficuumAS3.324 phyA gene from its clone vector, PUC18/phyAI.Then, through restrition digest and T 4 DNA polymerase ligation,we have cloned it to plant medium vector pBI121 and obtained pBI121/phyAⅡ particles with AS3.324 phyA contents.The next step is introducing pBI121/phyAⅡ into Agrobacterium tumefacien through freeze thaw method. Thus, the correct expressing vector—LBA4404/pBI121/phyA is achieved.Its reliability has been affirmed by antiboitic screening and PCR test. This expressing vector can be used for the study of transformating tobacco.

通过PCR方法从植酸酶基因克隆载体PUC18/ phyAI中扩增出Aspergillus.ficuumAS3 .32 4植酸酶 (phyA)基因 ,经酶切连接后克隆到植物中间载体pBI12 1,获得含AS3 .32 4植酸酶基因的质粒pBI12 1/ phyAⅡ。用冻融法将pBI12 1/ phyAⅡ导入表达载体根癌农杆菌LBA44 0 4中。经过抗生素筛选、PCR检测 ,证明得到了正确表达载体———LBA44 0 4/pBI12 1/ phyAⅡ ,此表达载体可用于植酸酶基因转化烟草的研究

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关植酸酶基因的内容
在知识搜索中查有关植酸酶基因的内容
在数字搜索中查有关植酸酶基因的内容
在概念知识元中查有关植酸酶基因的内容
在学术趋势中查有关植酸酶基因的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社