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文库检测
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  “文库检测”译为未确定词的双语例句
     The detecting result indicate that the size of recombinations were between 0.5kb and 3kb. So a good expressed library was successfully constructed.
     文库检测结果表明,该cDNA文库的插入片段介于0.5kb~3kb之间。
短句来源
     RESULTS: Eight ESTs of CYP450 genes includin g CYP1B1, CYP2F1, CYP2J2, CYP4B1, CYP4F12, CYP5A(TBXAS1), CYP20A1, and CYP51A1 w ere detected in non-cancerous nasopharynx cDNA library, among these CYP4B1 exhi bited the highest expression level with 16 copies of ESTs.
     结果:cDNA文库检测的CYP450基因EST(ExpressedSequenceTag)有CYP1B1、CYP2F1、CYP2J2、CYP4B1、CYP4F12、CYP5A(TBXAS1)、CYP20A1和CYP51A1,其中CYP4B1的EST拷贝数最高,达16个拷贝;
短句来源
     Methods: The cDNA library of Fungi(E 20) were constructed using the SMARTTM cDNA library construction kit. After having constructed the cDNA library, the titer and the recombination rate of the unamplified library were detected and then amplified.
     方法 :用SMARTTMcDNA文库构建试剂盒构建 (E - 2 0 )菌的cDNA文库 ,检测未扩增文库滴度和重组率后 ,进行文库的扩增 ,并检测扩增文库的质量。
短句来源
     Positive expression o f fourteen CYP450 genes including CYP1A1, CYP1B1, CYP2A6, CYP2A13, CYP2B6, CYP2C 8, CYP2C9, CYP2D6, CYP2E1, CYP2F1, CYP3A4, CYP3A5, CYP3A7, and CYP4B1 were detec ted by RT-PCR, among these, CYP1B1, CYP2B6, and CYP4B1 had also been detected i n the cDNA library.
     RT-PCR检测表达的CYP450基因有CYP1A1、CYP1B1、CYP2A6、CYP2A13、CYP2B6、CYP2C8、CYP2C9、CYP2D6、CYP2E1、CYP2F1、CYP3A4、CYP3A5、CYP3A7、CYP4B1,其中CYP1B1、CYP2B6、CYP4B1已经在文库检测有表达。
短句来源
     Result All 34 cDNAs showed negative to flavivirus and California serogroup virus primers;
     结果黄病毒属引物、加利福尼亚血清组病毒引物对34份cDNA文库检测结果均为阴性;
短句来源
  相似匹配句对
     THE CONSTRUCTION AND TESTING OF cDNA LIBRARY OF B.alboglabra
     芥蓝cDNA文库的构建及文库质量检测
短句来源
     99.9%.
     经检测文库覆盖率达到99.9%。
短句来源
     spectroscopy detection.
     能谱检测
短句来源
     After that, evaluated the effect of EPCs adhesion after Ad.
     检测Ad.
短句来源
     The length of inserted DNA fragment was determined with PCR and the inserted DNA fragment wascloned with T vector method.
     DNA文库
短句来源
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Objective To search new genes related to human esophageal cancer(EC) for revealing carcinogenesis mechanism and genetic susceptibility of EC. Methods Three normal esophageal epithelia(including 1 tumor adjacent tissue) and two primary squamous cell carcinomas collected from high incidence family in Lin xian county were studied using technique of mRNA differential display. The differential fragments were sequenced and identified by RT PCR assay. Results (1)Eighteen differential fragments were isolated...

Objective To search new genes related to human esophageal cancer(EC) for revealing carcinogenesis mechanism and genetic susceptibility of EC. Methods Three normal esophageal epithelia(including 1 tumor adjacent tissue) and two primary squamous cell carcinomas collected from high incidence family in Lin xian county were studied using technique of mRNA differential display. The differential fragments were sequenced and identified by RT PCR assay. Results (1)Eighteen differential fragments were isolated and identified, 13 of which were expressed in normal esophageal epithelia but not in EC(assigned as normal esophageal gene, NEG), while 5 were expressed in EC but not in normal esophageal epithelia(assigned as mutated esophageal gene, MEG). (2)Four NEG fragments were not homologous to the known sequences in the public database of GenBank (of NLM in USA). These 4 fragments were assigned as esophageal cancer related gene (ECRG) 1 to 4. (3)The remaining 14 fragments were homologues to 12 known genes or gene fragment. Their role in EC remains unclear. (4) Using RT PCR technique, the expression of ECRG between normal epithelia and EC was significantly different. (5) All 4 ECRG genes were expressed in cDNA libraries derived from normal fetal brain, adult brain, liver, kidney, testis, bone marrow and skeletal muscle. (6) In the 20 cancerous and tumor adjacent tissues obtained from the liver, lung, breast, colo rectum and endometria, ECRG1 and ECRG2 was not detected by RT PCR, while ECRG3 was highly expressed. For the ECRG4 the expression was much stronger in tumor adjacent tissues than in cancerous tissues. Conclusion ECRG 1 and ECRG2 may contribute to the causation and progression of the EC in Lin xian, and may be candidates of tumor suppressor genes.

目的分离人原发性食管组织中新的相关基因,揭示食管癌的易感性与癌变原理。方法用高效、灵敏的mRNA差异显示技术,以2例正常食管上皮、1例癌旁上皮、2例高癌家族的食管癌组织互为对照,通过对其基因表达的比较,找出差异条带,进行RTPCR鉴定和DNA序列分析。结果(1)在实验中,分离、鉴定了18个差异片段,其中包括正常组织表达而肿瘤不表达的正常食管基因(normalesophagealgene,NEG)13个,肿瘤表达而正常组织不表达的突变食管基因(mutatedesophagealgene,MEG)5个。(2)4个NEG片段在GenBank数据库中没有发现同源的已知基因或片段,将其分别命名为食管癌相关基因1~4(esophagealcancerrelatedgene,ECRG1~4)。(3)其他14个cDNA片段分别与GenBank中的12个已知基因或片段同源,这些基因在食管癌中的作用还有待于进一步研究。(4)通过RTPCR鉴定,已初步证明上述4个ECRG片段在正常食管上皮组织和食管癌中的表达有差异。(5)通过对7个人正常组织的cDNA文库检测表明,这4个ECRG片段在胎脑、成人脑、肝、肾、睾丸、骨...

目的分离人原发性食管组织中新的相关基因,揭示食管癌的易感性与癌变原理。方法用高效、灵敏的mRNA差异显示技术,以2例正常食管上皮、1例癌旁上皮、2例高癌家族的食管癌组织互为对照,通过对其基因表达的比较,找出差异条带,进行RTPCR鉴定和DNA序列分析。结果(1)在实验中,分离、鉴定了18个差异片段,其中包括正常组织表达而肿瘤不表达的正常食管基因(normalesophagealgene,NEG)13个,肿瘤表达而正常组织不表达的突变食管基因(mutatedesophagealgene,MEG)5个。(2)4个NEG片段在GenBank数据库中没有发现同源的已知基因或片段,将其分别命名为食管癌相关基因1~4(esophagealcancerrelatedgene,ECRG1~4)。(3)其他14个cDNA片段分别与GenBank中的12个已知基因或片段同源,这些基因在食管癌中的作用还有待于进一步研究。(4)通过RTPCR鉴定,已初步证明上述4个ECRG片段在正常食管上皮组织和食管癌中的表达有差异。(5)通过对7个人正常组织的cDNA文库检测表明,这4个ECRG片段在胎脑、成人脑、肝、肾、睾丸、骨髓及骨胳肌?

BACKGROUND &OBJECTIVE: The etiology of nasopharyngeal carcinoma (N PC) is associated with environmental and hereditary factors. Xenobiotics from en vironment must be activated to derive carcinogens. Several cytochrome P450 (CYP4 50) metabolic enzymes participate to the activation of pre-carcinogens, and the genetic polymorphism of those genes is associated with metabolic polymorphism a nd susceptibility to cancer. We performed a preliminary investigation on the xen obiotics metabolism of human nasopharynx by...

BACKGROUND &OBJECTIVE: The etiology of nasopharyngeal carcinoma (N PC) is associated with environmental and hereditary factors. Xenobiotics from en vironment must be activated to derive carcinogens. Several cytochrome P450 (CYP4 50) metabolic enzymes participate to the activation of pre-carcinogens, and the genetic polymorphism of those genes is associated with metabolic polymorphism a nd susceptibility to cancer. We performed a preliminary investigation on the xen obiotics metabolism of human nasopharynx by determining the expression of CYP450 genes in NPC and non-cancerous nasopharynx tissues. METHODS: The following two methods were used: (1)A cDNA library from the mixed RNA sample of seven non-ca ncerous nasopharynx tissues was generated, followed by clone sequencing and bioi nformatics analysis. (2)RNA of 14 NPC and 8 non-cancerous nasopharynx tissues w ere reversely transcribed, and the expression of CYP450 genes in those samples w as determined by PCR amplification. RESULTS: Eight ESTs of CYP450 genes includin g CYP1B1, CYP2F1, CYP2J2, CYP4B1, CYP4F12, CYP5A(TBXAS1), CYP20A1, and CYP51A1 w ere detected in non-cancerous nasopharynx cDNA library, among these CYP4B1 exhi bited the highest expression level with 16 copies of ESTs. Positive expression o f fourteen CYP450 genes including CYP1A1, CYP1B1, CYP2A6, CYP2A13, CYP2B6, CYP2C 8, CYP2C9, CYP2D6, CYP2E1, CYP2F1, CYP3A4, CYP3A5, CYP3A7, and CYP4B1 were detec ted by RT-PCR, among these, CYP1B1, CYP2B6, and CYP4B1 had also been detected i n the cDNA library. A total of 19 CYP450 genes expression were detected in NPC a nd non-cancerous nasopharynx tissues, and the expression levels of CYP1B1, CYP2 C8, CYP2C9, CYP2D6, CYP3A5, and CYP4B1 were higher than those of the other genes . CONSLUSION: The expression of a great number of CYP450 genes was detected in h uman nasopharynx, some of which might participate to the activation of pre-carc inogen in human nasopharynx. Contribution of these genes to the risk of NPC need s further investigation.

背景与目的:鼻咽癌(nasopharyngealcarcinoma,NPC)的发生与环境及遗传因素密切相关。环境中各种前致癌物需经代谢酶包括细胞色素P450(CytochromeP450,CYP450)的活化才具有致癌作用。本研究拟检测鼻咽癌及鼻咽非癌组织中的CYP450基因表达,以了解与鼻咽部组织中致癌物代谢活化有关的CYP450基因。方法:利用下述两种方法检测CYP450基因的表达:(1)混合7例鼻咽非癌组织的RNA构建cDNA文库,进行克隆测序及生物信息学分析;(2)逆转录14例鼻咽癌组织及8例鼻咽非癌组织的RNA为cDNA,以PCR扩增检测CYP450基因的表达。结果:cDNA文库检测的CYP450基因EST(ExpressedSequenceTag)有CYP1B1、CYP2F1、CYP2J2、CYP4B1、CYP4F12、CYP5A(TBXAS1)、CYP20A1和CYP51A1,其中CYP4B1的EST拷贝数最高,达16个拷贝;RT-PCR检测表达的CYP450基因有CYP1A1、CYP1B1、CYP2A6、CYP2A13、CYP2B6、CYP2C8、CYP2C9、CYP2D6、CYP...

背景与目的:鼻咽癌(nasopharyngealcarcinoma,NPC)的发生与环境及遗传因素密切相关。环境中各种前致癌物需经代谢酶包括细胞色素P450(CytochromeP450,CYP450)的活化才具有致癌作用。本研究拟检测鼻咽癌及鼻咽非癌组织中的CYP450基因表达,以了解与鼻咽部组织中致癌物代谢活化有关的CYP450基因。方法:利用下述两种方法检测CYP450基因的表达:(1)混合7例鼻咽非癌组织的RNA构建cDNA文库,进行克隆测序及生物信息学分析;(2)逆转录14例鼻咽癌组织及8例鼻咽非癌组织的RNA为cDNA,以PCR扩增检测CYP450基因的表达。结果:cDNA文库检测的CYP450基因EST(ExpressedSequenceTag)有CYP1B1、CYP2F1、CYP2J2、CYP4B1、CYP4F12、CYP5A(TBXAS1)、CYP20A1和CYP51A1,其中CYP4B1的EST拷贝数最高,达16个拷贝;RT-PCR检测表达的CYP450基因有CYP1A1、CYP1B1、CYP2A6、CYP2A13、CYP2B6、CYP2C8、CYP2C9、CYP2D6、CYP2E1、CYP2F1、CYP3A4、CYP3A5、CYP3A7、CYP4B1,其中CYP1B1、CYP2B6、CYP4B1已经在文库检测有表达。总共有19个CYP450基因在鼻咽癌及鼻咽非癌组织表达,表达较高的基因有CYP1B1、CYP2C8、CYP2C9、CYP2D6、CYP3A5和CYP4B1。结论:鼻咽部组织表达CYP450基因种类较多,其中包括与前致癌物代谢活化相关的基因。

Objective To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang.Method Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang,which were made into cDNA pools with pd(N)6 primer through RT-PCR method.Then PCR was used to detect viral nucleotide sequence from cDNA. Result All 34 cDNAs showed negative to flavivirus and California serogroup virus primers;but nairovirus and primets derived from Xinjiang hemorrhagic fever virus...

Objective To disclose the species and distribution of tick-borne arboviruses in the southern part of Xinjiang.Method Totally 5045 ticks were collected from 36 collecting sites of 23 places in the southern Xinjiang,which were made into cDNA pools with pd(N)6 primer through RT-PCR method.Then PCR was used to detect viral nucleotide sequence from cDNA. Result All 34 cDNAs showed negative to flavivirus and California serogroup virus primers;but nairovirus and primets derived from Xinjiang hemorrhagic fever virus had amplified and yielded some obvious bands corresponding to the nucleotide sequences of Xinjiang hemorrhagic fever virus.A phylogenetic analysis was done to the obtained partial sequences of L and S segments. Conclusion Nucleotide sequences of Neither flaviviruses nor California serogroup viruses were detected from the samples.However partial L segment sequence was first reported in China.Phylogenetic analysis of partial L and S segments disclosed the molecular characteristic of Xinjiang hemorrhagic fever virus.

目的了解新疆南部地区蜱传虫媒病毒的种类和分布情况。方法在新疆南部地区的23个采集地36个采集点,采集蜱标本5045只,分别建立蜱标本cDNA文库,用PCR方法检测标本可能携带的病毒。结果黄病毒属引物、加利福尼亚血清组病毒引物对34份cDNA文库检测结果均为阴性;内罗毕病毒属引物和新疆出血热病毒巢式引物在巴楚县蜱标本中检测到新疆出血热(XHF)病毒核酸序列,对检测到的XHF病毒L和S片段进行系统进化分析。结论对新疆南部地区5045只蜱进行四种六对引物的PCR检测,未检出黄病毒属和加利福尼亚血清组病毒核酸序列,获得新疆出血热病毒L和S片段的核酸序列,研究了其分子流行特征。

 
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