Study on the High Activity Mechanism, the Adsorption Properties and the Hydrolysis Kinetic of Xylanase from Sp.E-86 Strain
Construction and Expression of Hybrid Gene Encoding Thermostable Xylanase and Property of Hybrid Enzyme
Characterization and Application of a Thermostable Xylanase from the Thermophilic Thermomyces Lanuginosus
嗜热真菌Thermomyces lanuginosus耐热 木聚糖酶性质及应用研究
Immobilization of Xylanase from Streptomyces Olivaceoviridis E-86 and Its Application for Xylooligosaccharide Production
Study on Anti-nutritive Mechanism of Wheat NSP and Application of Xylanase Supplemented in Wheat-based Diet for Broilers
A stable β-xylanase producer-mutant L10-IGA was filtrate out through treatment with UV,NTG,UV on wild strain L-10. The β-xylanase and CMCese activities of L10-IGA were 124.45 IU/mL,and 1.08 IU/mL, respectively.
经筛选和诱变 ,选育出 1株产 β 木聚糖酶活力较高 ( 1 2 4.45IU/mL) ,而羧甲基纤维素酶活力较低( 1 .0 8IU/mL)的突变株L1 0 IGA。
The cDNA sequence of β-xylanase gene (xynB) was cloned from Aspergillus niger UV-11. It was inserted into the yeast expression vector and the recombinant plasmid pAX2 was obtained.
用重叠延伸PCR方法从黑曲霉 (Aspergillusniger)UV 11的基因组DNA中克隆出 木聚糖酶的cDNA基因 ,构建了由酵母乙醇脱氢酶 (ADH1)启动子和终止子引导表达、 木聚糖酶自身信号肽引导分泌、rDNA序列介导的酵母整合型分泌表达质粒pAX2。
Study on Selective Breeding and Culture of Bacteria Producing Stable β-xylanase
The suitable medium for Penicillium sp.m8 producing extracellular xylanase consisted of wheat stram hemicelluloses 40g/L, (NH-4)-2SO-4 4.5g/L,KH-2PO-4 1.0g/L,MgSO-4·7H-2O 0.5g/L,NaCl 0.3g/L,Tween80 3.0g/L,CaCO-3 1.0g/L.
青霉菌m8产胞外 木聚糖酶的适合培养基 (g/L) :含麦草粉 4 0 ,(NH4) 2 SO44.5 ,KH2 PO41.0 ,MgSO4·7H2 O 0 .5 ,NaCl 0 .3,Tween80 3.0 ,CaCO3 1.0。
Studies of Comditions of Culture for Peaicilliumsp. m8 and Characters of Extracellular Xylanase
Identification of B. licheniformis H-1 and Properties of Extracellular Xylanase
When the xylan extracted at 80 ℃ was used as carbon source, an orthogonal experiment was carried out for optimization of Volvariella volvacea fermentation to produce extracellular xylanase.
The extracellular xylanase from Streptomyces sp.Strz-6 was purified 32.5 times by salting out,ion-exchange and molecular sieve chromatography. The specific activity and recovery of purification xylanase were 498 u/mg and 46.6 % respectively.
The Study on High Activity Xylanase Production from Streptomyces olivaceovirdis E-86 by Different Inducer
不同诱导物对Streptomyces olivaceovirdis E—86产高活性 木聚糖酶的研究
Their xylanase production(from(0.895) U_1 to 3.239 U_1) and cellulase production(0.391 U_2 and 465 U_2 respectively) were tested respectively.
还分别测定了它们产 木聚糖酶(从0.895 U1到3.239 U1)和纤维素酶活性(分别为0.391 U2和0.465 U2)。
Fermentation Conditions of Xylanase Production by Streptomyces sp. D21
Xylanase production by Aspergillus niger JY021 was carried out under liquid fermentation process. The culture medium and the cultivation conditions were optimized as follows: liquid concentration of corn cobs 2%, glucode 0.3%, corn starch 1%, wheat bran 0.2%, Mandels nutrient 20%, Mandels microelement 0.2%, 1M citrate buffer 5%, Tween 80 0.3%, pH 4.8, shaking flask speed 200rpm.
采用Aspergillus niger JY021在液态培养条件下产 木聚糖酶,优化后的工艺条件为:玉米芯木聚糖液2%,葡萄糖0.3%,玉米浆1%,麸皮0.2%,Mandels营养盐浓缩液20%,Mandels微量元素浓缩液0.2%,1M柠檬酸缓冲液5%,Tween80 0.3%,pH4.8。
nitrogen source and other factors on xylanase production of Pseudomonas sp. WLUN024 were studied. The results showed the optimal conditions for enzyme formation were wheat bran 6%, (NH_4)_2So_4 0.8%, K_2HPO_4 0.4%, inoculum size 5%- 10%. its enzyme activity reached 600IU/mL under 37℃ in 36h.
研究了碳源、氮源以及其他因子对 木聚糖酶高产菌WLUN024（Pseudomonas sp．）产酶的影响，结果表明在麸皮6g／L、（NH_4）_2SO_4 0．8g／L、K_2HPO_4 0．49／L、接种量5％～10％的条件下，37℃培养36h，其 木聚糖酶活力可达600IU／mL。
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The possibility of using xylanase preparations for hydrolyzing hemicelluloses in a non-bleached kraft pulp in order to facilitate its bleaching was studied.
The effects of enzymatic preparations of fungal and bacterial origins were examined, and the optimal conditions for xylanase activity were determined.
UV spectroscopy demonstrated that the treatment of kraft pulp with enzymatic preparations containing xylanase facilitated the subsequent removal of lignin and increased the brightness by 5%.
Purification and Characterization of endo-(1→4)-β- xylanase fromGeotrichum candidum 3C
A method of purification of endo-( 1 → 4)-β- xylanase (endo xylanase; EC 184.108.40.206) from the culture liquid ofGeotrichum candidum 3C, grown for three days, is described.
Purification and Characterization of endo-(1→4)- β-xylanase fromGeotrichum candidum 3C
A method of purification of endo-( 1 → 4)- β-xylanase (endoxylanase; EC 220.127.116.11) from the culture liquid ofGeotrichum candidum 3C, grown for three days, is described.
Cloning of Penicillium canescens Endo-1,4- β-xylanase Gene and Construction of Multicopy Strains
The complete gene xylA that encodes endo-1,4- β-xylanase secreted byPenicillium canescens was cloned and sequenced.
Induction of the Synthesis of Endo-1,4- β-xylanase and β-Galactosidase in Original and Recombinant Strains of the Fungus Penicill
An extracellular xylanase produced by a Mexican Aspergillus strain was purified and characterized.
The main difference between the production of xylanase by Streptomyces and corynebacteria was the low level of processing of the mature extracellular xylanase by B.
Strain HC1 cells grown on rice bran hemicellulose as a sole carbon source inducibly produced extracellular xylanase and intracellular glycosidases such as β-d-glucosidase and β-d-arabinosidase.
This enzyme contains carbohydrates in a noncovalent manner, indicating that this extracellular xylanase, is not a glycoprotein.
Studies on the extracellular xylanase activity of some thermophilic actinomycetes