连接效率 efficiency of ligation(6) ligation efficiency(7) joint efficiency(0)   efficiency of ligation      It is important to improve the efficiency of ligation reaction, especially for joining of blunt-ended DNA which is universal applicated, however, with lower efficiency. In our study, We draw a conclusion that the mol-ratio of vector and insert?      在平末端连接反应中 ,为了提高连接效率 ,研究载体与片段的分子量比、载体与片段的摩尔比、连接反应体积、连接反应的温度、连接反应时间长短等条件对连接效率的影响 ,发现这些因素都会直接影响连接效率。 短句来源      The Study to Improve the Efficiency of Ligation of Blunt-ended DNA      提高平末端连接效率的研究 短句来源      It was estimated that the efficiency of ligation is about 65%.      经测定DNA的连接效率在65％左右。 短句来源      Methods:After being isolated from virulent and avirulent strain of S. mutans respectively, the intact and high-pure genomic DNAs were digested with restriction enzyme AluⅠ. The digested DNA of the avirulent strain ligated with an adaptor was used as tester DNA, and that of the virulent strain as driver DNA. Then the suppression subtractive hybridization(SSH) was carried out, the efficiency of ligation and subtraction were detected respectively.      方法:从一对c血清型变形链球菌高、低毒力株中提取基因组DNA,AluⅠ酶切,以低毒力株的DNA酶切产物为testerDNA,高毒力株的DNA酶切产物为driverDNA,testerDNA连接接头后与driverDNA进行抑制消减杂交,并检测连接效率与消减效率。 短句来源      Methods After being isolated from virulent and avirulent strain of S.mutans respectively,the intact and high-pure genomic DNA was digested with three appropriate four-base-cutting restriction endonucleases to produce fragments of optimal length. The digested DNA of the virulent strain ligated with adaptor was used as tester DNA,and that of the avirulent strain as driver DNA. Then the suppression subtractive hybridization was carried out,and the efficiency of ligation and subtraction detected respectively.      方法从一对c血清型变形链球菌高、低毒力株中提取基因组DNA,用四碱基限制性内切酶酶切,以高毒力株的DNA酶切产物为tester DNA,低毒力株的DNA酶切产物为driver DNA,tester DNA连接接头后与driver DNA进行抑制消减杂交,并检测连接效率与消减效率。 短句来源 更多          ligation efficiency      Increase of DNA Ligation Efficiency by Temperature Cycles      用温度循环提高DNA连接效率(简报) 短句来源      Methods EMCs from the first branchial arch of E9.5 and dental papilla cells isolated from lst lower molar tooth germs of E16.5 were cultured atthe same conditions. EMCs qua driver,SSH cDNA libraries of up-ragulated genes was constructed. Analysis of total RNA qualities,cDNA Taq 1digestion,adaptors ligation efficiency,subtraction efficiency and PCR products were all studied.      方法在相同的条件下,体外培养E9.5、E16.5第一鳃弓外胚间充质细胞和牙乳头细胞,采用抑制消减杂交技术(SSH),以第一鳃弓外胚间充质细胞为驱赶子(Driver),建立其牙向分化上调基因消减文库,并检测接头连接效率、消减效率等指标。 短句来源      Methods EMCs from the first branchial arch of E9.5 and dental papilla cells isolated from 1st lower molar tooth germs of E16.5 were cultured at the same conditions. EMCs qua tester,SSH cDNA libraries of down-ragulated genes was constructed. Analysis of total RNA qualities,cDNA Taq Idigestion,adaptors ligation efficiency,subtraction efficiency and PCR products were all studied.      方法在相同的条件下,体外培养E9.5、E16.5第一鳃弓外胚间充质细胞和牙乳头细胞,采用抑制消减杂交技术(SSH),以第一鳃弓外胚间充质细胞为检测子(Tester),建立其牙向分化下调基因消减文库,并检测接头连接效率、消减效率等指标。 短句来源      METHODS: EMCs from the first branchial arch of E9.5 and dental papilla cells isolated from 1st lower molar tooth germs of E16.5 were cultured at the same conditions. EMCs qua driver, SSH cDNA libraries of up-ragulated genes was constructed. Analysis of total RNA qualities, cDNA Taq I digestion, adaptors ligation efficiency, subtraction efficiency and PCR products were all studied.      方法:在相同的条件下,体外培养E9．5、E16．5第一鳃弓外胚间充质细胞和牙乳头细胞,采用抑制消减杂交技术(SSH),以第一鳃弓外胚间充质细胞为驱赶子(driver),建立其牙向分化上调基因消减文库,并检测接头连接效率、消减效率等指标。 短句来源      The ligation efficiency of tester DNA with adaptor was at least higher than 25 percent. The subtraction efficiency of suppression subtractive hybridization confirmed the success in enrichment of differential genes between virulent and avirulent strain of S.mutans. In the subtracted group,the appearance time of the 23S rRNA gene both in tester and driver DNA was later than that in the unsubtracted group by six cycles.      tester DNA与接头的连接效率大于25%,抑制消减杂交后消减效率检测显示,同时存在于tester与driver DNA中的23S rRNA基因在消减组中出现时间较未消减组晚6个循环,将消减PCR产物克隆后,挑取96个转化子,构建高毒力株特异的基因文库。 短句来源 更多          “连接效率”译为未确定词的双语例句      When pH 3.5 and 20 mmol / L for HEPES, pH 5.6 and 5 mmol / L for Na3PO4, the LRAs were 108.01 and 76.78 μg/mg respectively.      Na3PO4的pH为5.6,浓度为5mmol/L时,抗体连接效率分别为108.01和76.78μg/mg。 短句来源      The optimal coupling efficiency of 71% was obtained while antibody density in liposome was 106 μg antibody/μmol phospholipids (as initial antibody/PDP-PEG-HSPE=1∶10).      当初始Ab/PDP-PEG-HSPE=1∶10时,脂质体与抗体的连接效率为71%,抗体密度为106μgAb/μmolPL。 短句来源      Many buffers could be used for antibody conjugation onto BMPs, and under the normal buffer pH and concentration, the LRAs of 8 buffers were 46.28±0.58 ~ 90.83±1.64 μg/mg.      PBS、HEPES、Tris-HCl、MOPS、VBS以及磷酸盐等溶液可用于抗体连接到BMPs体系中,在通常使用的缓冲液pH和浓度下,几种溶液的抗体连接效率变化范围为46.28±0.58￣90.83±1.64μg/mg; 短句来源      LRAs depended upon the amount of antibody and BMPs, when BMPs decreased from 5.0 mg to 0.1 mg, and antibody increased from 10 μg to 300 μg, the LRAs increased gradually. When BMPs at 0.1 mg /mL and antibody at 300 μg/mL were used, the LRA reached to 762.37 μg/mg.      反应体系中,BMPs用量由5mg降到0.1mg,抗体用量由10μg增加到300μg时,抗体连接效率逐渐上升,当BMPs用量为0.1mg,抗体用量为300μg时,抗体连接效率达762.37μg/mg。 短句来源      The results indicated that the HBTU/HOBt/DIEA strategy was the best method and the optimum reaction conditions for the esterification were set at temperature 40℃,molar ratio 3:1,reaction time 4h using NMP/DCM(1:7,v/v) solution.      实验结果表明了采用HBTU/HOBt/DIEA方法的连接效率最高。 对反应的优化结果为:NMP/DCM体积比为1:7,体系、摩尔比为3:1,每次反应时间4 h,温度40℃。 短句来源 更多        查询“连接效率”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   efficiency of ligation The efficiency of ligation amounted to from 60 to 90%. 例句来源       However, the average efficiency of ligation could be potentially enhanced by optimizing the LDR conditions. 例句来源         ligation efficiency Applying 20 LDR cycles and assuming 100% ligation efficiency for each cycle, one would expect 60 000 LDR products. 例句来源       Single-base mutation at the third or fourth position away from the ligation point only showed less than 5% of the initial ligation efficiency. 例句来源       The recombinant enzyme exhibits extremely high activity and high ligation efficiency. 例句来源         joint efficiency This note determines necessary and sufficient conditions for a production technology to exhibit both input translation homotheticity and output translation homotheticity without invoking joint efficiency. 例句来源       In the cooperative structure, the joint efficiency which is modelled as the average of the seller's and buyer's efficiency scores is maximized, and both supply chain members are evaluated simultaneously. 例句来源       This proposed architecture attempts to address the search for joint efficiency for negotiating agents in large and complex problem spaces using a co-evolutionary method. 例句来源       While aluminium-lithium alloys are fusion weldable with gas metal arc, gas tungsten arc and electron beam processes, they suffer from problems of weld porosity, heat-tearing cracking, poor penetration and low joint efficiency. 例句来源       Mechanical tests revealed the electronbeam weld to have lower strength, elongation and joint efficiency. 例句来源       更多                    Four designed DNA fragments which contained a sequence for transcr-iptional termination were synthesized by the DNA Synthesizer Model 381 A (Applied Biosystems). The procedures for separation, purification and liga-tion of these synthetic DNA fragments were described. It was estimated that the efficiency of ligation is about 65%.             研究设计了一段带终止信号的DNA序列,通过DNA合成仪分为四个片段合成。本文介绍经DNA合成仪合成DNA片段后的处理,纯化及连接过程.经测定DNA的连接效率在65％左右。 文摘来源          In this paper, the weldability of Aluminum-Lithium alloysusing gas metal arc welding, gas Tungston arc welding, electron beamwelding, laser beams welding. and the effects of postweld heat treatment onjoin efficiency are discribed.             本文叙述了铝锂合金使用金属弧焊,气体钨弧焊,电子束焊及激光束焊的可焊性及热处理对焊接后连接效率的影响。 文摘来源          The efficiency of transformation is inversely related to the size of the plasmid,This becomes a limiting factor when the plasmid exceeds 15kb in size.In this case,it is important to improve the efficiency of ligation reaction,especially for jollling of blunt-ended DNA which is universal applicated, however, with cower efficiency. In our tests, Addition of Hpa i,or EcoR V which generated linear plasmids with bluntend to the reaction system make a notiable improvement in the efficiency of the ligation, which ...             转化效率与质粒大小成反比，而当质粒大于１５ｋｂ时，转化效率将成为限制因素，此时提高连接效率极为必要，特别是对于具有普遍性而效率又低的平末端连接。我们在连接反应体系中，加入相应的产生平末端质粒载体的限制性内切酶ＨＰａＩ、或ＥｃｏＲＶ，与不加ＨＰａＩ、或ＥｃｏＲＶ的连接反应相对照，连接产物转化大肠杆菌ＪＭ１０９后，产生的转化子比率分别为１９．９％和５．６％（ＰＦＰ７），３２．６％和９．７％（ｐＦＢ１７５），２４．３％和３．６％（ｐＦＨ１２），３７．９％和１０．３％（ｐＦＰ２６），且从转化株中分离的质＃ＤＮＡ经电泳、酶切鉴定为目的产物。ＨｐａＩ、或ＥｃｏＲＶ的存在极有利于插入片段连接到载体上，同时抑制载体自身环化或寡聚化，大大提高了平末端连接效率。 文摘来源   << 更多相关文摘         相关查询: 抗体连接效率 连接 连接( 提高多表连接效率 效率 连接绳 通气效率 连接隔框 最优效率 连接边界 发热效率 诱杀效率  对查询结果不满意

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