助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   电洗脱 的翻译结果: 查询用时:0.47秒
图标索引 在分类学科中查询
所有学科
生物学
农作物
公安
更多类别查询

图标索引 历史查询
 

电洗脱
相关语句
  electric elution
     A fibrinolytic enzyme was purified from the crude extract of Bs-2,a strain of Bacillus sp . ,through ammonium sulphate fractional precipitation,Sephadex G-75 gel filtration,Q-Sepharose anion exchange chromatography,preparative PAGE and electric elution.
     芽孢杆菌 2 # (Bs- 2 )的发酵液经过硫酸铵分级沉淀、SephadexG - 75凝胶过滤层析、Q -Sepharose阴离子交换层析、电泳制备和电洗脱 ,获得一种具有纤溶活性的蛋白酶 ;
短句来源
     Methods The recom-binant bacterial strain containing Tau gene was induced with IPTG, and the expressed product was purified by sonication, salting out with ammonium sulfate, ion exchange charomatography and electric elution, then purified by SDS - PAGE, non - denaturing PAGE, Western blot and amino acid sequencing of N - terminal.
     方法 将携带 Tau蛋白基因的工程菌株经IPTG诱导表达后,通过超声破碎、硫酸按盐析、离子交换层析、电洗脱等方法纯化Tau蛋白,以SDS-PAGE、非变性PAGE、Westem blot和N末端氨基酸测定等方法进行鉴定。
短句来源
     Rice genomic DNA had been isolated by improved CTAB method,and the Sau3AI partial-digestion products were recovered by electric elution with dialysis bag. After concentrated by n-butyl alcohol,the purified partial-digestion products were ligated with vector arms in the 1∶1 ratios.
     采用改良的CTAB法提取水稻基因组DNA,经Sau3AI部分酶切的产物经透析袋电洗脱法回收、正丁醇浓缩后,以1∶1的摩尔数比和载体臂连接.
短句来源
  electro-elution
     The recombinant proteins GST-E54-2 and GST-E54-4 were purified through denaturation and refolding of inclusion bodies and electro-elution assay.
     采用包涵体洗涤和电洗脱纯化重组蛋白GST-E54-2和GST-E54-4。
短句来源
     Recombinant proteins were purified by metal chelated affinity chromatography or by electro-elution. Western blot was used to detect the reactivity of these proteins with the convalescent sera of SARS patients.
     分别利用电洗脱或金属鳌合介质纯化重组蛋白,用蛋白印迹实验检测纯化蛋白对SARS病人恢复期血清的反应性,结果发现全长N蛋白活性最好,其余的末端缺失蛋白均无法达到同一活性水平。
短句来源
     The peptides were very stable to keep the antibacterial activity even kept in 95℃ for 120min and frozen-thawed for 10 times. A weak-acid antibacterial peptide MD_(7095) had been purified in high degree of purity by electro-elution,and was determined Mr 7095Da with MALDI-TOF-MS and pI 5.59 with IEF-PAGE. Peptide mass fingerprinting(PMF) analysis showed MD_(7095) was a novel bioactive peptide.
     通过电洗脱方法进一步纯化出抗菌肽MD7095,质谱测定其分子量7095Da,IEF电泳测得其等电点5.59,经肽质量指纹谱(PMF)鉴定为一新肽。
短句来源
  electrophoretic elution
     DNA Profiling from Degrative DNA by Polymerase Chain Reaction(PCR):Recovered the Larger DNA Fragments by Electrophoretic Elution
     降解DNA的PCR分型-电洗脱回收大片断DNA法
短句来源
     The protein bands between 10~30 kDa purified by SDS PAGE and electrophoretic elution were used in dot ELISA.
     采用电洗脱技术分离 10~ 30kDa蛋白组分 ,建立纯化抗原的dot ELISA血清学诊断斯氏狸殖吸虫病。
短句来源
  “电洗脱”译为未确定词的双语例句
     Purified rSj14-3-3 was prepared through SDS polyacrylamide gel electrophoresis ( SDS-PAGE) , electroelution, dialysis, then BALB/c mice were divided into 5 groups and immunized in rSj14-3-3 protein followed by challenging infection (the 1st, 2nd, and 3rd groups were immunized in 50μg, 100μg and 300μg antigen, respectively. The 4th, 5th groups were immunized in Freund's adjuvant and normal saline controls).
     用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)、电洗脱和透析的方法制备纯化的rSj14-3-3蛋白,分5组(第1、2、3组为rSj14-3-3蛋白50μg、100μg和300μg实验组,第4、5组分别为福氏佐剂和生理盐水对照组)免疫BALB/c小鼠并进行攻击感染实验。
短句来源
     An indirect ELISA method was established as to de tect the expression of HA1 fusion protein. Positive sera of H5, H7, H9 subtype A IV were detected by this indirect ELISA.
     电洗脱方法纯化表达HA1蛋白 ,建立间接ELISA方法 ,对感染AIVH7、H9、H5亚型AIV阳性血清进行检测。
短句来源
     By ultrafiltration with PM 10 Amicon Membrane, electrophoresis with Disc PAGE, and electrical wash out with Biotrap apparatus, D band protein was isolated from the SC SFCM.
     通过PM10超滤浓缩、Disc-PAGE和Biotrap电洗脱,从SC-SFCM中分离出D蛋白带,分子量在43~67Kd之间。
短句来源
     After the inclusion bodies of recombinant proteins were purified with washing buffer consisting of 2mol/L, 4mol/L and 8mol/L urea for several times, the recombinant proteins were purified by electrodialysis method.
     用含有2mol/L、4mol/L和8mol/L尿素的包涵体洗涤液多次洗涤包涵体后,用电洗脱方法对各个重组蛋白进行纯化,获得了理想的纯化效果。
短句来源
     Spleen-derived B cells from the BALB/c mouse immunized with the purified fusion protein (Eae-stx1/2B) were fused with SP2/0 myeloma cells and three hybridoma cell lines, named as 1G2, 3C6 and 1B10, were established by ELISA.
     融合蛋白采用凝胶分离电洗脱法回收纯化,用纯化的蛋白抗原免疫BALB/c小鼠,细胞融合后获得的3株杂交瘤细胞株1G2、3C6、1B10,能分别稳定分泌针对紧密素、志贺毒素Stx1和Stx2的单克隆抗体。
短句来源
更多       
查询“电洗脱”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  electro-elution
The major proteins were separated by SDS PAGE and purified by electro-elution.
      
We find that two CA proteins, which were partially purified by electro-elution treatment, can control the morphology of CaCO3 crystals and one of them is potentially involved in the process of biocalcification.
      
A "column-format" preparative electrophoresis device which obviates the need for gel extraction or secondary electro-elution steps is described.
      
For further analysis, this band was excised from the gel and the protein was collected by electro-elution.
      
One rabbit was injected with AtEm1 and column chromatography and electro-elution before being another one with AtEm6.
      


Using EMBL3 phage DNA as a vector, a perfect genomic library of Echinococcus granulosus from sheep from Xinjiang has been constructed, which contains 1.2×06 independent recombinants.The main procedure of construction comprised: 1. extraction of Echinococcus granulosus genomic DNA, 2. partial digestion of the extracted E. granulosus genomic DNA with restriction enzyme Sau3A1, 3. harvest of 15-23 kb DNA fragments by electric elution equipment, 4. preparation of EMBL3 phage vector DNA, 5. cleavage of EMBL3 vector...

Using EMBL3 phage DNA as a vector, a perfect genomic library of Echinococcus granulosus from sheep from Xinjiang has been constructed, which contains 1.2×06 independent recombinants.The main procedure of construction comprised: 1. extraction of Echinococcus granulosus genomic DNA, 2. partial digestion of the extracted E. granulosus genomic DNA with restriction enzyme Sau3A1, 3. harvest of 15-23 kb DNA fragments by electric elution equipment, 4. preparation of EMBL3 phage vector DNA, 5. cleavage of EMBL3 vector DNA with two restriction enzymes Bam HI and Eco RI to remove the central fragment, 6. ligation of the harvested 15-23 kb E. granulosus genomic DNA and the cleavaged vector DNA with T4 DNA ligase, 7. the package reaction of ligated recombinant DNA in vitro with the package protein and with Q359 strain (Spi-) to screen recombinants, 8. identification of the genomic library, 9. hybridization in situ by pSM889 probe, obtaining 6 positive recombinant clones. (Figs. 1-6)It was estimated that the E. granulosus genome was about 1.5×108 bp. A perfect E.g. genomic library is expected to contain 4.6×104 independent recombinants at minimum The construced genomic library has enough independent recombinants (1.2×106).This is the first time to establish an E. granulosus genomic library in China. Previous work showed that there existed differences in many aspects, including pathogenicity, among various isolates of E. granulosus from different hosts and areas. We plan to employ this library to screen the E. granulosus intraspecies DNA probe by hybridization in situ. This kind of probe is envisaged to be of advantage for epidemiological investigation of the hydatid disease in China. It also provides a base for researching E. granulosus at the molecular level.

细粒棘球绦虫DNA经Sau3AI部分水解,并用电洗脱方法从琼脂糖凝胶中回收15-23kb大小的片段。得到的片段与λ噬菌体置换载体EMBL3 DNA重组,采用体外包装系统构建了细粒棘球绦虫的基因库,获得了1.2×10~6个重组子。通过与探针的分子杂交,从库中分离到6个细粒棘球绦虫γDNA基因的阳性克隆。

Marck's disease virus gcnomic DNA clone BamHI-K_3 samples, which in the forms of the total recombinant plasmid DNA or only the BamHI-K_3 fragment purified by digestion, clectrophoresis and elcctroelution from the total recombinant plasmid, were labeled with nonradioactive digoxigenin respectively and used as probes for dctcction of Marek's disease virus DNA. The comparison of two probes for their specificities has indicated that the probe of the total recombinant plasmid DNA gave a strong cross-reaction to other...

Marck's disease virus gcnomic DNA clone BamHI-K_3 samples, which in the forms of the total recombinant plasmid DNA or only the BamHI-K_3 fragment purified by digestion, clectrophoresis and elcctroelution from the total recombinant plasmid, were labeled with nonradioactive digoxigenin respectively and used as probes for dctcction of Marek's disease virus DNA. The comparison of two probes for their specificities has indicated that the probe of the total recombinant plasmid DNA gave a strong cross-reaction to other heterogeneous DNA in the another kind of plasmid. When the elcctroclution-purificd BamHI-K_3 was used as probe, it gave specific reactions only to plasmid preparalion of homologous DNA clones or E. coli colonies with homologous DNA clones, it did not show any cross-reaction to other plasmid preparation without homologous DNA fragment.

鸡马立克病病毒基因组DNA的BanHI-K_3片断克隆的重组质粒DNA及从质粒上切下的经电泳和电洗脱提纯的BamHI-K_2DNA片断,在用非放射性Digoxigenin([dig])标记后,分别用作检测马立克病病毒DNA的探针。对两种探针特异性的比较表明,BamHI-K_3克隆的全质粒DNA探针对其它质粒来源的NDA仍表现出很强的交叉反应性,而用经电泳电洗脱提纯的BamHI-K_3DNA片断制备的探针,则仅对含同源DNA片断的质粒提取物或菌苔裂解物产生明显呈色反应,但对不含同源DNA的其它质粒DNA提取物几乎完全不呈现交叉反应。

We recovered DNA fragment digested by restriction endonuclease by using the DF-17 gel elec- trophoresis tanks.It was found that it is a simple,rapid and reliable method of recovery of DNA frag- ment.The DNA fragment may be further digested by restriction enzymes and nick-translated by DNase 1 and DNA polymerasel.

我们采用国产 DF-17型电洗脱槽回收了限制性内切酶酶切的 DNA 片段,证明该方法是一种简便、快速、可靠的回收核酸片段的方法.回收的 DNA 片段可进一步用于 DNA 克隆、探针标记和 DNA 分析.

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关电洗脱的内容
在知识搜索中查有关电洗脱的内容
在数字搜索中查有关电洗脱的内容
在概念知识元中查有关电洗脱的内容
在学术趋势中查有关电洗脱的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社