助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   系统诱导表达 的翻译结果: 查询用时:0.24秒
图标索引 在分类学科中查询
所有学科
植物保护
更多类别查询

图标索引 历史查询
 

系统诱导表达
相关语句
  “系统诱导表达”译为未确定词的双语例句
     Establishment of Conditional Expression of c-myc in Transgenic Mice and Tumor Model by the Tet-On System
     Tet-On系统诱导表达c-myc转基因小鼠及肿瘤模型的建立
短句来源
     Methods By E. coli expressing system, the GPC3 protein as antigen was induced, and the rabbit anti-GPC3 polyclonal antibody was prepared. The purified antibody was used to do Western blot analysis in 40 liver tissues (7 normal liver tissues,26 HCC liver tissues and 7 ICC liver tissues) and 4 cell lines ( HepG2,HuH-7,Hep3B, Hela).
     方法利用原核表达系统诱导表达了GPC3抗原并制备了特异性好的兔抗GPC3多克隆抗体,应用该纯化的抗体对40例组织(正常肝7例,肝细胞型肝癌26例,胆管细胞型肝癌7例)及4个细胞系(肝癌细胞系HepG2、HuH-7、Hep3B,非肝癌细胞系Hela)进行了蛋白印记杂交试验。
短句来源
     Establishment of transgenic mice conditional expressing c-myc gene by the Tet-on system
     Tet-on系统诱导表达c-myc转基因小鼠的建立
短句来源
     Analyes of the founder 123# binary transgenic mice line for SV40 TAg revealed that the transgenes could be regulated in the brain and the thymus gland by administration of doxycycline.
     利用Founder 123#转基因小鼠成功建立了Tet-on系统诱导表达SV4O T转基因小鼠脑成神经管细胞瘤模型。
短句来源
     Objective To construct a conditional expression vector containing esp1 in order to observe the effect on the ploidy in A_549 cells.
     目的观察肺腺癌A549细胞经Tet-on系统诱导表达esp1基因后其染色体数目的改变。
短句来源
更多       
  相似匹配句对
     The Chemical-inducible Expression System of Plant Genes
     植物的化学诱导表达系统
短句来源
     Eukaryotic inducible expression systems: advances in research
     真核细胞诱导表达系统研究进展
短句来源
     Establishment of 5-lipoxygenase inducible expression system
     5-脂氧合酶诱导表达系统的建立
短句来源
     P. Pastoris Expression System
     毕赤酵母表达系统
短句来源
     The expression was induced by IPTG.
     IPTG诱导表达
短句来源
查询“系统诱导表达”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  systemic induced expression
Wounding of 14-day seedlings gave rise to systemic induced expression mainly in the vascular tissue.
      


Objective To express HEV structural gene by using pichia pastoris expression system. Methods The chimeric gene segment HEV ORF 23 (an 800bp gene segement close to the HEV ORF2 C end and the whole ORF3 gene segement) of HEV structural gene is cloned into pPICZ α A pichia expression vector by double digest with Not Ⅰ and Xba Ⅰ. The recombinant plasmid pPICZ α A_HEV ORF 23 is transformed appropriate pichia pastoris strain by using electroporation and then the interest gene is integrated into the genome DNA by...

Objective To express HEV structural gene by using pichia pastoris expression system. Methods The chimeric gene segment HEV ORF 23 (an 800bp gene segement close to the HEV ORF2 C end and the whole ORF3 gene segement) of HEV structural gene is cloned into pPICZ α A pichia expression vector by double digest with Not Ⅰ and Xba Ⅰ. The recombinant plasmid pPICZ α A_HEV ORF 23 is transformed appropriate pichia pastoris strain by using electroporation and then the interest gene is integrated into the genome DNA by a single crossover. The recombinant pichia strains are selected for induced expression by methanol, purifying the expression products and doing western_blot detection. Results We obtain a 69kDa protein in the cell lysates after induced 72 hours under pH6.0 circumstance. The protein can be purified with His·Bind Resin, and the western_blot results is positive. Conclusions Using pichia pastoris expression system and His purification system, a soluble, high antigenic and immunogenic HEV recombinant protein can be obtained, and it makes a foundation for the development of the efficient hepatitis E diagnostic reagent and vaccine.

目的使用酵母表达系统表达戊型肝炎病毒结构蛋白基因。方法构建含戊型肝炎病毒(HEV)结构蛋白嵌合基因HEVORF23(由HEVORF2C端800bp片段与ORF3全基因组成)的重组表达质粒pPICZαA_HEVORF23,并通过电转移的方法将该基因整合入甲醇营养型酵母的基因组DNA中,利用甲醇进行诱导表达,表达产物经纯化后,进行Western印迹鉴定。结果pH6.0环境下诱导培养72h后,在细胞沉淀中获得一个分子量大约为69kDa的可溶性目的蛋白,纯化蛋白经Western印迹检测后发现能与戊型肝炎患者血清发生较强的特异性免疫反应。结论采用酵母表达系统诱导表达可以获得抗原性较强的HEV重组表达抗原,为进一步开发研制有效的诊断抗原和疫苗奠定了基础。

Objective To construct a conditional expression vector containing esp1 in order to observe the effect on the ploidy in A_549 cells.Methods Tet-on regulating plasmid was transferred into A_549 cells under the Dox induced Tet-on regulating system and stable expression of Tet-on was established in A_549 cells through G418 selection.The response plasmids of recombinant PTK-Hyg and PTRE-EGFP-esp1 were transferred into positive A_549 /Tet-on cells,and stable expression of esp1 was established through hygromycin and...

Objective To construct a conditional expression vector containing esp1 in order to observe the effect on the ploidy in A_549 cells.Methods Tet-on regulating plasmid was transferred into A_549 cells under the Dox induced Tet-on regulating system and stable expression of Tet-on was established in A_549 cells through G418 selection.The response plasmids of recombinant PTK-Hyg and PTRE-EGFP-esp1 were transferred into positive A_549 /Tet-on cells,and stable expression of esp1 was established through hygromycin and green fluorescence selection.Eight single cellular clones were taken and cultivated in amplification.Positive A_549 /Tet-on/TK-Hyg/IRE-EGFP-esp1 cells,whose expression were induced by Dox showed well dose-response in expression of esp1 with different concentration and time of Dox induction,were selected by reverse transcriptase-polymerase chain reaction(RT-PCR).The changes of chromosome in different A_549/Tet-on/TK-Hyg/IRE-EGFP-esp1 cell clone which were induced different time were detected by the traditional colchicines method and flow cytometry(FCM).Results The best Dox concentration induced high esp1 expression is from 100ng/ ml to 200ng/ ml.The esp1 could express after 1h induce by Dox and the high expression occured after 48h induction,after that the expression of esp1 begin to descent.The chromosome of positive A_549 /Tet-on/TK-Hyg/IRE-EGFP-esp1 cells had no changes until were induced 24h by Dox.Conclusion The up-regulated expression of esp1 has certain inhibitory effect on abnormal division of tumor chromosome,so it has potential value in the treatment of cancer.

目的观察肺腺癌A549细胞经Tet-on系统诱导表达esp1基因后其染色体数目的改变。方法利用PTet-on基因表达调控系统,先后将调控质粒Ptet-on和反应质粒PTK-Hyg与PTRE-EGFP-esp1以合适的比例用FuGENE 6脂质体共转染入A549细胞,经G418和潮霉素筛选成活并带有绿色荧光的细胞为转染成功细胞,挑选8个单细胞克隆并扩增培养,加入强力霉素诱导esp1表达。用RT-PCR检测不同浓度强力霉素诱导以及强力霉素诱导不同时间的esp1的表达量,并采用传统秋水仙素方法以及流式细胞技术检测诱导不同时间段A549/Ptet-on/TK-Hyg/PTRE-EGFP-esp1细胞克隆染色体数目的改变。结果100~200ng/ml浓度范围的强力霉素诱导esp1表达量最高,诱导1h即可有esp1基因的表达,至48h表达量到达高峰之后逐步减少。在诱导24h后逐步出现染色体数目减少。结论上调esp1基因对肿瘤细胞异常染色体有一定的抑制作用,对肿瘤治疗有潜在的应用价值。

Objective To study the expression of GPC3 protein in liver cancer and HCC cell lines by anti-GPC3 polyclonal antibody,and to evaluate the possibility of GPC3 protein as a novel tumor marker for liver cancer. Methods By E. coli expressing system, the GPC3 protein as antigen was induced, and the rabbit anti-GPC3 polyclonal antibody was prepared. The purified antibody was used to do Western blot analysis in 40 liver tissues (7 normal liver tissues,26 HCC liver tissues and 7 ICC liver tissues) and 4 cell lines (...

Objective To study the expression of GPC3 protein in liver cancer and HCC cell lines by anti-GPC3 polyclonal antibody,and to evaluate the possibility of GPC3 protein as a novel tumor marker for liver cancer. Methods By E. coli expressing system, the GPC3 protein as antigen was induced, and the rabbit anti-GPC3 polyclonal antibody was prepared. The purified antibody was used to do Western blot analysis in 40 liver tissues (7 normal liver tissues,26 HCC liver tissues and 7 ICC liver tissues) and 4 cell lines ( HepG2,HuH-7,Hep3B, Hela). Results A main protein band of 70 kD which corresponding to GPC3 core protein was found both in liver cancer tissues and HCC cell lines HepG2, HuH-7,Hep3B by Western blot test. It was found that normal liver tissues did not express GPC3 protein, but in 26 HCC patients, the percentage of GPC3 protein expressed in liver cancer tissues and its corresponding non-cancerous liver tissues was 84. 6% (22/26) and 30. 8% (8/26) .respectively. Among 7 ICC patients, only 2 patients had GPC3 protein expression. Conclusion GPC3 protein is distributed widely in the tissues of liver cancer,which may be a novel tumor marker for liver cancer.

目的制备聚糖蛋白3(glypican-3,GPC3)抗体并应用其对肝癌组织及细胞系中GPC3蛋白表达进行研究,探讨GPC3蛋白作为肝癌潜在的新的肿瘤标志物的可能性。方法利用原核表达系统诱导表达了GPC3抗原并制备了特异性好的兔抗GPC3多克隆抗体,应用该纯化的抗体对40例组织(正常肝7例,肝细胞型肝癌26例,胆管细胞型肝癌7例)及4个细胞系(肝癌细胞系HepG2、HuH-7、Hep3B,非肝癌细胞系Hela)进行了蛋白印记杂交试验。结果Western blot结果显示,肝癌组织及肝癌细胞系HepG2、HuH-7、Hep3B细胞系中有相对相对分子质量约70000的蛋白主带,与GPC3的核心蛋白带相对分子质量大小相一致;正常肝组织不表达GPC3蛋白,肝细胞型肝癌中癌组织(HCC)及癌旁组织表达GPC3蛋白的比例分别为84.6%(22/26)及30.8%(8/26),7例胆管细胞型肝癌(ICC)中有2例癌组织及其旁组织表达GPC3。结论GPC3蛋白广泛存在于肝癌组织中,很可能成为一种新的肝癌肿瘤标志物。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关系统诱导表达的内容
在知识搜索中查有关系统诱导表达的内容
在数字搜索中查有关系统诱导表达的内容
在概念知识元中查有关系统诱导表达的内容
在学术趋势中查有关系统诱导表达的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社