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癌细胞诱导分化
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     Studying the Inducing Differentiation Effects of 1.25-DihydroxyvitaminD_3 on Pancreatic Cancer Line
     1,25-(OH)_2D_3对胰腺癌细胞诱导分化的研究
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     PHENOTYPE TRANSITION OF B 7-2 EMBRYONAL CARCINOMA CELLS DURING INDUCED DIFFERENTIATION WITH HEXAMETHYLENEBISACETAMIDE
     B7-2胚胎性癌细胞诱导分化过程中的表型转变
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     cAMP Receptor Protein and Induced Differentiation in Gastric Cancer Cell Line
     cAMP受体蛋白与胃癌细胞诱导分化的实验研究
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     HMBA could induce marked apoptosis and potentiated differentiation in human mucoepidermoid carcinoma cell line . The mechanism could be activating transcription regulation of P 21(cip1/waf1) and P 53 genes.
     提示 HMBA通过激活转录因子 P2 1(cip1/ waf1) ,P53的表达而发挥对粘液表皮样癌细胞诱导分化、促进凋亡的作用
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     Studies on the Relation Between the Nuclear Matrix and DNA Synthesis During Induced Differentiation of Gastric Carcinoma Cells
     胃癌细胞诱导分化中核骨架与DNA合成的关系
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     The Changes of Function on Colorectal Cancer Cells induced by Retinoic Acid
     经维甲酸诱导分化的大肠癌细胞的功能改变
     INDUCTION OF DIFFERENTIATION OF HUMAN GASTRIC CANCER CELL LINE BY RETINOIC
     维甲酸对人胃癌细胞诱导分化的研究
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     Effects of cinnamic acid on differentiation of MGC-803 cells
     肉桂酸对胃腺癌细胞诱导分化的实验研究
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     Induced differentiation and gap junction formation of murine embryonal carcinoma cells
     鼠胚胎癌细胞诱导分化和间隙连接形成
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     ALL TRANS RETINOIC ACID INDUCED DIFFERENTIATION AND APOPTOSIS OF COLORECTAL CANCER CELL LINE LoVo
     ATRA诱导结肠癌细胞株LoVo分化与凋亡
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examethylene Bizacetamide content was determined by high performance liquid chromatography using ananalytical column(15 × 0. 46cm i.d. )packed DU PONT ZORBAX-ODS(5μm)and a UV detector at 220nm andMeOH-H_2O(50 : 50 ) as eluent.The quantitative determination was performed with caffeine as the internalstandard.The methed is satisfactory for the quality control of hexamethylene bizacetamide.

高效液相色谱法测定六亚甲基二乙酰胺注射液含量徐丽婷,赵玉喜,谢景文,谢廷泉(兰州军区总医院兰州730050)1前言癌细胞诱导分化剂是当今世界上正在研究的一种治疗癌症的新途径。六亚甲基二乙酰胺(HexamethyleneBizaceteamide,HM...

Human mucoepidermoid carcinoma cell line MEC 1 cells were induced by hexamethylene bisacetamide(HMBA) in this study.The effective concentration of HMBA was 1mmol/L.The cell growth kinetics,DNA content,AgNORs of cells were performed.Results indicate that the human mucoepidermoid carcinoma cell line MEC 1 cells tend to be come mature cells by the treatment of HMBA,implying that HMBA can induce differentiation of MEC 1 cells in vitro .The effective concentration is rather low and the differentiation effects...

Human mucoepidermoid carcinoma cell line MEC 1 cells were induced by hexamethylene bisacetamide(HMBA) in this study.The effective concentration of HMBA was 1mmol/L.The cell growth kinetics,DNA content,AgNORs of cells were performed.Results indicate that the human mucoepidermoid carcinoma cell line MEC 1 cells tend to be come mature cells by the treatment of HMBA,implying that HMBA can induce differentiation of MEC 1 cells in vitro .The effective concentration is rather low and the differentiation effects are obvious.It is worthy of further study.

用六亚甲基二乙酞胺(HMBA)作为分化诱导剂,对人涎腺粘液表皮样癌细胞系MEC-1细胞进行体外诱导(诱导浓度1mmol/L),探讨HMBA对粘液表皮样癌细胞的诱导分化作用。实验经细胞生长动力学、细胞DNA含量、核仁区嗜银蛋白染色指标观察,显示在HMBA作用下MEC-1细胞有向成熟细胞分化的趋势,而且诱导所需浓度低,分化作用明显,有进一步研究价值。

Abstract The differentiation of CNE--2 Cells separately grown on the glass dishes (G) and on the artificial basement memdrane (ABM) induce by all--trans--retinoid acid 10--7mol/L was condu cted over nine days. TIP results showed that the inhibited prolifelation, the reduction of the soft agar--dependent anchorage and the transplantation in nude mice occurred in the cells grown on G but not on ABM, However, the Golgi 's body, the rough endoplasmic reticulum (RER), the cell surface microvilli , intercelular...

Abstract The differentiation of CNE--2 Cells separately grown on the glass dishes (G) and on the artificial basement memdrane (ABM) induce by all--trans--retinoid acid 10--7mol/L was condu cted over nine days. TIP results showed that the inhibited prolifelation, the reduction of the soft agar--dependent anchorage and the transplantation in nude mice occurred in the cells grown on G but not on ABM, However, the Golgi 's body, the rough endoplasmic reticulum (RER), the cell surface microvilli , intercelular junctions such as tight junction, interdigitation, desmosome and acinic cavity construction were noted much more in the ABM cells than that in G. These findings suggested. 1) The ABM antagonized the inhibition role of RA on the cell proliferation and the reduction of malignant phenotypes, but helped the RA with the radonstruction of intercellular structure 2) The research work on differentiation of cancerous cells induced in vitro should. be made under the cultUlal system system mimetic to the enviroment in vivo so that the evaluation of the medicinal effects of agents inducing the differentiation in vivo will be more reliable .Keyworks: nasopharyngeal carcinoma, artificial basement membrane; retinoid acid, differentiation

用全反维甲酸(RA)10-7mol/L对生长在玻璃皿(G)和人工基膜(ABM)上的鼻咽癌细胞株(CNE-对细胞进行9d的诱导分化。结果显示生长在G上的癌细胞增殖被抑制,软琼脂内集落形成和裸鼠体内生长等恶性表型减弱,生长在ABM的细胞则不受影响,但其表面微绒毛丰富,细胞间的紧密连接、交指状连接、桥粒和腺腔样结构多见,高尔基器,粗面内浆网发育较好。表明不同的培养基质对RA的生物学作用有不同的影响,ABM有拮抗RA抑制癌细胞增殖和去恶化的作用,但有助于癌细胞间结构的重建和细胞器的发育,提示在体外进行癌细胞诱导分化时应在近似体内环境的培养系统中进行,才能更确切地评价分化诱导剂的体内治疗反应。

 
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