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   血清制备 的翻译结果: 查询用时:0.901秒
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血清制备
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  serum preparation
     A simple method for bovine serum preparation
     一种简易的小牛血清制备方法
短句来源
     METHODS: ① Drug serum preparation: Rats in Xingnao Qizhi drug serum 25.42, 12.71, 6.35 and 3.18 g/kg groups were exposed through gastric lavage to xingnao qizhi of corresponding dosages (composed of wolfberry fruit, grassleaved sweetflag rhizome, chuanxiong rhizome and jü luo), while normal saline was given by gastric lavage to rats in control group in dosage of 10 mL/kg, twice a day at an interval of 12 hours for 3 consecutive days. One hour after the last administration, femoral blood samples were collected when rats were deeply anaesthetized and serum was separated.
     方法:①药物血清制备:醒脑启智药物血清25.42,12.71,6.35,3.18g/kg组按相应剂量以醒脑启智药物(枸杞子、石菖蒲、川芎、橘络等)灌胃给药,对照血清组以生理盐水10mL/kg灌胃,均为2次/d,每天灌胃间隔12h,连续给药3d,末次给药后1h麻醉状态下股动脉采血,分离血清。
短句来源
  serum was prepared
     The HSC were separated and cultured after rat models of hepatic fibrosis were established with CCl4,and drug serum was prepared. The influences of drug serum on the proliferation of HSC in all the groups were detect by MTT at different time with the wave length of 450 nm.
     采用大鼠CCl4造模后肝星形细胞分离、培养,药物血清制备,MTT法检测法各组血清对HSC不同时相增殖的影响,酶标仪波长为450nm。
短句来源
     HSCs in SD rats were separated and cultured,and meanwhile,the drug serum was prepared. The expression of typeⅠcollagen,type Ⅲcollagen and TIMPs of HSCs were measured by immunohistochemistry,and analyzed quantitively with image analysis system(IAS).
     采用SD大鼠肝星形细胞分离、培养,药物血清制备,免疫组化检测实验各组肝星形细胞中Ⅰ,Ⅲ型胶原及TIMPs的表达经图像分析系统定量分析。
短句来源
  “血清制备”译为未确定词的双语例句
     Methods A rat ATG model was established by tail intravenous injection with rabbit anti-Thy-1 serum.
     方法尾静脉注射兔抗Thy1血清制备大鼠ATG模型;
短句来源
     Methods 5 ml blood was extracted from 151 patients and healthy blood donators,then DNA was extractedand amplyfied by PCR from sera.
     方法取门诊及住院患者和健康献血员151例,采静脉血5 ml,分离血清制备DNA模板,进行PCR扩增;
短句来源
     Preparation of Anti-HBe Monoclonal Antibody from HBeAg Positive Human Serum and its Identification
     用e抗原阳性血清制备抗-HBe单克隆抗体及其鉴定
短句来源
     Different drug serums were added to HSC separated from SD male rat for culture to examine expression of TGF β1 and PDGF of HSC by immunohistochemistry,and analyze quantitatively the outcome with image analysis system(IAS).
     采用SD大鼠肝星形细胞分离、培养、药物血清制备,免疫组化检测各实验组血清对肝星形细胞分泌TGF-β1,PDGF的影响,并经图像分析系统作定量分析。
短句来源
     Part3. Protective function of XNQZ Drug-containing serum on the cell PC12 injury.
     第四部分 XNQZ药物血清对缺氧诱导的PC12细胞凋亡及凋亡基因的影响1 TUNEL法检测药物血清制备、PC12细胞培养、缺氧损伤诱导方法同前。
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  相似匹配句对
     Blood sample was then centrifuged and serum was retained.
     血清
短句来源
     The content of P. C.
     血清P.
短句来源
     Preparing of serum reagent of alloantibody
     制备同种抗体血型血清试剂的体会
短句来源
     PREPARATION OF HUMAN SPECIFIC ANTI-ALBUMIN ANTISERUM
     特异性抗人白蛋白血清制备
短句来源
     PREPARATION OF METHYL TERTIARY BUTYL ETHER
     甲基叔丁基醚的制备
短句来源
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  serum preparation
Serum preparation, however, is a notorious source of artefactually high or low levels of fibrin(ogen) degradation products, and is not suitable for the determination of coagulation products.
      
We randomly assigned 20 term neonates with venous haematocrit (Hct)>amp;gt;0.65 l/l to PET with either a serum preparation (BISEKO) or Ringer solution.
      
Altered activity in cultured cells caused by contaminants in tubes widely used for blood collection and serum preparation
      
Effect of leukocytic serum preparation on hematopoietic cells in vitro
      
After 2 weeks of acclimatization, 10 mL of blood was collected from the marginal ear vein of each rabbit and pooled for preimmune serum preparation.
      
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  serum was prepared
3)-containing serum was prepared with serum pharmacological technique, and then was applied to react on mesangial cells cultured in fetal calf serum (FCS) and cells cultured in FCS plus lipopolysaccharide.
      
A heterologous rabbit anti-rat macrophage serum was prepared which showed cross-reactions with both rat erythrocytes and rat thymocytes.
      
After sequential absorptions a specific antimacrophage serum was prepared, which did not show agglutination or cytotoxicity for rat thymocytes or lymph node cells.
      
At the end of the 14-d period, serum was prepared from both control and clenbuterol-treated chickens, and was then employed as a component of cell culture media at a final concentration of 20% (v/v).
      
A GFP-Dirc1 fusion protein was expressed in vitro and a polyclonal anti-Dirc1 peptide serum was prepared.
      
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The present paper deals with a general review of the researchworks on plant viruses and virus diseases which have been donein the people's Republic of China since 1950.Topics included are theplant virus diseases hitherto known to China including variousisolates and strains, reports of new entities,electron microscopicalstudies, investigations on the preparation of antisera, interactionsbetween viruses in their host plants, some studies of RNA and ofvirus infection in relation to the physiological changes in...

The present paper deals with a general review of the researchworks on plant viruses and virus diseases which have been donein the people's Republic of China since 1950.Topics included are theplant virus diseases hitherto known to China including variousisolates and strains, reports of new entities,electron microscopicalstudies, investigations on the preparation of antisera, interactionsbetween viruses in their host plants, some studies of RNA and ofvirus infection in relation to the physiological changes in plant,studies of insect vectors,epidemiology, disease resistance and methodsof control. 136 literatures are cited.

文章综述了我国自1950年以来有关植物病毒及病毒病害的研究概况,其中包括我国已知的植物病毒病,植物病毒的分离物及株系,新病毒的报道,电子显微镜的观察,抗血清的制备及血清反应研究,在寄主植物中病毒之间的干扰,有关病毒核酸的研究,病毒侵染对植物生理的影响,介体的研究,病毒病的发病及流行,植物对病毒的抗性以及农作物病毒病的防治等。文章共引述了136篇参考资料。

In experiments on the preparation of rice dwarf virus antisera,a more feasible process for obtaining partially purified antigen for immu- nization was developed,essentially using polyethylene glycol precipita- tion in conjunction with low speed centrifugation as alternative to using ultracentrifuge equipment.Separate tests on optimizing factors reve- aled that concentration of PEG added to 6% (w/v),NaCl 0.3M,phos- phate buffer to 0.03M and pH at 6.8 were optimum rates for the par- tially purified antigen preparation.Small-tubing...

In experiments on the preparation of rice dwarf virus antisera,a more feasible process for obtaining partially purified antigen for immu- nization was developed,essentially using polyethylene glycol precipita- tion in conjunction with low speed centrifugation as alternative to using ultracentrifuge equipment.Separate tests on optimizing factors reve- aled that concentration of PEG added to 6% (w/v),NaCl 0.3M,phos- phate buffer to 0.03M and pH at 6.8 were optimum rates for the par- tially purified antigen preparation.Small-tubing precipitin test techni- que and agar gel diffusion technique were employed in antigen-antibody reaction tests,and gave good results indicating that the antisera so pre- pared produced fairly high titres.Thus,to illustrate,antiserum ob- tained from a single injection for immunization from most of the rabbit gave a titre of 1:640;antigera obtained from rabbits injected 3 or 4 times gave a titre of 1:2560.A titre of 1:5120 was obtained in one case with a rabbit injected twice,and in another case with a rabbit injected 6 times.

在进行水稻普矮病毒(RDV)的抗血清制备上,在缺乏超速离心机设备条件下,采用了聚乙二醇(Polyethlene Glycol 即 PEG-MW6000)结合用低速离心机达到抗原的部份纯化。试验结果说明在抗原制备上,PEG 的浓度达6%(重量/容积),NaCl0.3M,磷酸缓冲液0.03M,pH6.8,是最适条件。抗原—抗体反应用小管沉淀反应技术及琼脂扩散技术,均获得良好效果,反映制备出的 RDV 抗血清具有相当高的效价。例如:免疫化过程上,以抗原注射家兔一次的,抗血清效价,在多数的试验中为1/640;经注射三次或四次的,效价高的达1/2560,有一只兔经注射一次,另一只注射六次,其抗血清效价高达1/5120。

In the production of antiserum against the flacherie virus, a simple process was developed for purifying the antigen for immunizing the rabbits. The process consisted essentially of extaracting the flacherie larva midguts in phosphate buffer, clarifying with chloroform, precipitating the virus with ammonium sulphate, followed then by centrifugation at 3000 rpm. for 30 min. The pellet obtained was suspended in small amount of phosphate buffer at pH7.2, and the suspension underwent 3000 rpm centrifugation for...

In the production of antiserum against the flacherie virus, a simple process was developed for purifying the antigen for immunizing the rabbits. The process consisted essentially of extaracting the flacherie larva midguts in phosphate buffer, clarifying with chloroform, precipitating the virus with ammonium sulphate, followed then by centrifugation at 3000 rpm. for 30 min. The pellet obtained was suspended in small amount of phosphate buffer at pH7.2, and the suspension underwent 3000 rpm centrifugation for 60 min. The supernatant obtained was used as purified antigen for immunization. The virus suspension purified with this process showed,in ultraviolet spectrum, maximum absorption at 260,271am, showed spherical particles in electron micrograph with no noticeable extraneous material. Antiserum derived from the purified antigen showed specific reaction pattern in counter immuno-electrophoresis test, in which no cross-reactivity was observed.

在软化病毒抗血清制备中,开展了供免疫用抗原的简易纯化工作。主要步骤是将软化病蚕中肠在磷酸缓冲液及氯仿中抽提澄清,硫酸铵盐析,结合3000rpm离心30分钟,沉淀用少量pH7.2的磷酸缓冲液悬浮并经3000rpm离心60分钟,上清液即为供免疫用的纯抗原。用上法纯化所得病毒悬液,在紫外分光光度计上测得的吸收高峰为260,271nm;电子显微镜观察中除了球形病毒粒子外没有看到其他物质;用此法纯化为抗原所得到的抗血清在对流免疫电泳试验中特异性典型,且无交叉反应出现。

 
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