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The most favourable solid seed-medium and cultural conditions were screenedout through orthogonal experiments.Under such suitable conditions the numberof spores produced was increased 7.5 times more than those on Gause's No.1synthetic medium while the culture time needed only 4 days,i.e.beingshortened by at least 7~10 days.The curve of the spore number produced at different times showed that therewere several generations of spores produced on the same agar-slants;each gene-ration's spores possessed different... The most favourable solid seed-medium and cultural conditions were screenedout through orthogonal experiments.Under such suitable conditions the numberof spores produced was increased 7.5 times more than those on Gause's No.1synthetic medium while the culture time needed only 4 days,i.e.beingshortened by at least 7~10 days.The curve of the spore number produced at different times showed that therewere several generations of spores produced on the same agar-slants;each gene-ration's spores possessed different characteristics in antibiotical production.The fermentation flasks inoculated with the first or the second generationalspores would produce more nanchangmycin A or more effective insecticidalantibiotics(the fraction B)respectively. 本研究进行了南昌链霉菌孢子培养基和培养条件的筛选。筛选到的培养基和培养条件,使南昌链霉菌每支斜面孢子达5亿多,是高氏培养基产孢量的7.5倍,培养时间缩短了7~10天。同时初步探讨了南昌链霉菌在固体培养基上孢子的消长变化及对摇瓶发酵产素的影响,发现该菌在固体培养基上能周期性地产生多代孢子;摇瓶发酵,接种第一代孢子南昌霉素 A 产量最多,接种第二代孢子则高效杀虫组分 B 的效价最高。 The adsorption and desorption extraction processes of Midecamycin in the fermentation broth with macroreticular adsorbents in the production process were discussed.The material and experimental conditions such as, adsorbents, eluants and other relevant factors were selected and determined in the laboratory.Results showed that, (l)the static capacity of Midecamycin onto the MD-A adsorbent is as high as 6.7 × 10~4u/g of resin, from fermentation broth with a concentration of 1,500 u/ml,and is 40% higher than that... The adsorption and desorption extraction processes of Midecamycin in the fermentation broth with macroreticular adsorbents in the production process were discussed.The material and experimental conditions such as, adsorbents, eluants and other relevant factors were selected and determined in the laboratory.Results showed that, (l)the static capacity of Midecamycin onto the MD-A adsorbent is as high as 6.7 × 10~4u/g of resin, from fermentation broth with a concentration of 1,500 u/ml,and is 40% higher than that of CAD-40 adsorbent reported ia the literature) (2) the dynamic capacity of Midecamycin onto the ME)-A adsorbent is 1.32×10~5 u/g of resin; (3) a kind of ketone was used as eluant with elution rate of 93%. 本文主要介绍用大网格吸附剂从发酵液中提取麦迪霉素的吸附与解吸过程的研究,包括吸附剂和解吸剂的选择、吸附与解吸条件的筛选等。结果表明:选用MD-A型吸附剂,在发酵液浓度为1500u/ml左右时,对麦迪霉素的静态吸附容量高达6.7×10~4u/g抽干树脂,比以前报道过的CAD-40吸附剂高出40%左右,动态吸附容量可达1.32×10~5u/g干树脂;所筛选到的酮类洗脱剂其解吸率可达93%以上。 Antioxidative conditions in the process of extracting tea polyphenols have been studied. The experimental results proved that the percentage of tea polyphenols oxidized could be apparently controlled by adding inorganic antioxidant in the neutral solutions. The antioxidative activity increased in the following order: KI 本文报道了茶多酚制备(工艺)过程中抗氧化条件的筛选,研究结果表明在中性介质中添加无机抗氧化剂能明显抑制茶多酚氧化,其抗氧化效应序列MST>MSO_4>Na_2S_2O_3>KI。MST对人体无害且价廉,它的应用为茶多酚商品化开发提供诱人前景。
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