Comparing with traditional methods of pellets,the quality of the freezing semen remarkably improved,the motility of the thawed semen increased from 35.45% to 57.88%(P<0.01),the intact acrosomes rate elevated from 42.25% to 78.24%(P<0.01).
The changes of trace element Zn in semen were observed on 60 patients with particular fewness or feelbleness of semen by building kidney and nursing the liver.
Results The method of goat semen freezing and thawing was as follows: the washed spermatozoa were resuspended in 20% egg yolk diluted to 2×10~8/mL as the final sperm cell concentration,then followed by 4 h equilibration period at 4℃ before freezing,and goat semen frozen in pellet form and thawed at 40℃ by TALP buffer.
Compared with that in the controlled group, the sperm viability in the experimental group was decreased significantly on D1 PI and D1.5 PI (P>amp;lt;0.05).
The semen of Beagle was collected with massage method, its successfulrate was 71.4%. The ejaculatory semen volume was 2.67 mL, and its pH value was 6.0-6.5. The whole course of ejaculation may be divided into 3 stages, the spermatozoawere mainly in the semen ejaculated in2nd stage. The spermatozoon density was 2.105×10~8/mL. The vitality of fresh semen was 0.8 to 0.9. After dilution with two kinds ofdiluents and freezing in liquid nitrogen for 14-60 days, the rate of incubation vitalitieswere 0.415 and 0.42...
The effects of semen extender A(S. E. A) and semen extender B (S. E. B) on sperm motility after freezing and thawing was tested with 20 semen samples of holstein bulls. S. E. A contained 10 g/1000 ml fructose,while in SEB Glucose was used instead of fructose at the same molarity. Other components and their quantities in the two extenders remained identical. The idea of this trial was to compare the effects of fructose and glucose in the extenders.Paired contrast was adopted. The semen from the same sample w...
In this test, three kinds of diluents, three kinds of glycerine concentration, three kinds of yolk concentration and three kinds of starting frozen temperature were used in freezing and defrosting the sperm of goat at designed proccdure,checking gradually the activity of sperm and living sperm percentage. We observed the spcrm by scrccning supcrmicrostructutc with EM after collection of spcrm. before freezing and after defrosting. All data were analysed by single factor analysis of significant difference. I...