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突变株分离
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  mutant isolation
     In view of these, the authors give here a comprehensive commentary on some common techniques in mutant isolation, enzyme assay and signal detection and their progress recently.
     居于此,文中综合评述在突变株分离、酶检测和信号探测等方面,目前的一些常用技术及其最新进展.
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  “突变株分离”译为未确定词的双语例句
     each with its own features and colors, were segragated and cloned from the VM3 generation mutants and were named 85-1 and 85-2 respectively.
     通过无性繁植,已从VM_1代突变株分离纯化出两个花色各具特点的突变系,分别编号为“85-1”和“85-2”。
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     A mutant 880103 was obtained from parent strain A03 by an improved selection procedure of mutant.
     麦迪霉素产生菌生米加链霉菌A03菌株经过多次诱变筛选及抗自身代谢物突变,得到了产量正变株; 通过推理筛选的方法,应用初级代谢产物生物合成途径的调节突变株,分离得到了一主要组分麦迪霉素A_1高于对照菌株的优良菌株880103。
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     The growth of G. oxydans and fermentation process are studied. The results show that the rate of producing 2-KG of strain 201A is higher than that of the strain now used(201C) ,the conversion rate being about 80%.
     在对维生素C生产菌选育过程中,发现一株葡萄糖酸杆菌正向突变株,分离纯化后,获得优良菌株201A,经摇瓶培养,做生长曲线测定,发现前期生长及产酸明显高于生产菌株201C,经流加发酵工艺培养,可缩短发酵周期8h,山梨糖生成2—酮基—L—古龙酸的摩尔转化率达80%。
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  相似匹配句对
     Isolation of Pathogenic Mutants of Magpaporthe Grisea
     稻瘟病菌致病突变株分离
     Isolation of Pathogenic Mutants of Magnaporthe Grisea
     稻瘟病菌致病突变株分离
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     Separation of Mixtures with Same Boiling Point
     分离共沸混合物
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     Thermal Diffusion Separation
     热扩散分离
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     Compared with its initial strain A.
     突变株A.
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  mutant isolation
Mutant isolation techniques with plant tissue cultures
      
Establishment of haploid and diploid cell lines with stable chromosome number is important for mutant isolation and protoplast fusion.
      
Procedures for mutant isolation and scoring, maintenance of stocks, mating, sporulation, complementation, tetrad and random spore analysis have been developed for these inbred strains.
      
Mutant isolation was facilitated by directed mutagenesis and a mutant bacterial host which is unusually restrictive for missense mutations in gene 32.
      
However a rapid and simple route to mutant isolation was obtained, which could allow the use of other λ-related genetic techniques in several important species which, to date, have not been genetically manipulated.
      
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This experiment lasted from 1985 to 1988. Mutants of Bougainvillea spectabilis induced with 60Co γ rays were used for selecting new varieties of different colors.The results indicated that root-sprouting cuttings, treated with a radiation dose of 3KR and 3.5KR, produced mutants of various colors in the VM1 generation. Cuttings treated with 3KR brought about a mutant from which a scarlet and an orange-yellow colored flowers grew, itsmutation rate being 0.74%. Two other mutants, with orange-yellow and red-yellow...

This experiment lasted from 1985 to 1988. Mutants of Bougainvillea spectabilis induced with 60Co γ rays were used for selecting new varieties of different colors.The results indicated that root-sprouting cuttings, treated with a radiation dose of 3KR and 3.5KR, produced mutants of various colors in the VM1 generation. Cuttings treated with 3KR brought about a mutant from which a scarlet and an orange-yellow colored flowers grew, itsmutation rate being 0.74%. Two other mutants, with orange-yellow and red-yellow flowers respectively, emerged from the 3.5KR treated cuttings, rate 1.64%.Through vegetative reproduction, two mutant strains. each with its own features and colors, were segragated and cloned from the VM3 generation mutants and were named 85-1 and 85-2 respectively.The new varieties reproduced to the VM3 generation, exhibiting a long blossoming period, stable characters, unique colors as well as a high appreciation value. All these varieties are being propagated and popularized now.

本研究于1985~1988年,用~(60)Coγ射线诱发重瓣叶子花突变,选育新的花色品种。试验结果表明,辐照剂量为3KR和3.5KR处理的发根插条,在VM_1代均获得不同花色突变株,其中3 KR处理的出现一株分别有鲜红和橙黄色的变异,突变率为0.74%;3.5KR处理的出现两株分别为橙黄色和红黄嵌合花色变异,突变率为1.64%。通过无性繁植,已从VM_1代突变株分离纯化出两个花色各具特点的突变系,分别编号为“85-1”和“85-2”。经继代繁殖到VM_3代,表现性状稳定,花期特长,花色艳丽,具有较好的观赏价值,目前正在加速繁育推广应用。

Chlorophyll mutants of different type were obtained from indica type temperature sensi-tive genic male·sterile rice(cv. 2177S )by using“Co γ-rays irradiation. The total chloro-phyll mutation frequency reached to 0.26%in M2 generation. However only about 4. 50%of these mutants could survived.Among them,33 heritable chlorophyll mutant lines wereeasily distinguished,and were screened and studied.The mutants either showed chlorosis oryellowing or expressed only at seedling period or persisted all growth cycle. The...

Chlorophyll mutants of different type were obtained from indica type temperature sensi-tive genic male·sterile rice(cv. 2177S )by using“Co γ-rays irradiation. The total chloro-phyll mutation frequency reached to 0.26%in M2 generation. However only about 4. 50%of these mutants could survived.Among them,33 heritable chlorophyll mutant lines wereeasily distinguished,and were screened and studied.The mutants either showed chlorosis oryellowing or expressed only at seedling period or persisted all growth cycle. The expressionof mutant character was stable under different environment.It is suggested that they are use-ful as the marker traits in two-line hybrid rice.Moreover,the agreconomical traits of most ofthese lines changed in different levels compared with the parent line 2177S.Every mutationline seemed to be controlled by one recessive gene as the F1 plants of reciprocal crosses be-tween mutant and 2177S showed normal leaf color. And the ratio of green plants/mutantplants was 3:1 in the segregated F2 population.

用60Coγ射线辐照粑型温敏核不育系2177S,获得了一批常见的叶绿素突变体。M2代按苗计,叶绿素总突变频率为0.262%,其中只有4.50%可以存活并遗传,从中筛选出33个综合性状优良的籼型温敏核不育叶绿素突变系。经鉴定,这些叶绿素突变受基因控制,比较稳定,有的只在苗期表达并逐渐转为正常,有的则在全生育期表达。与2177S相比,农艺性状都有不同程度变化,但温敏不育特性基本不受影响。遗传分析表明,所有的突变体与亲本正反交时,杂种F1均为绿色植株,杂种F2群体中,正常绿色株与对应叶绿素突变株分离比值符合3:1。证明这类突变是由1对隐性细胞核基因所控制。

Objective To isolate and identify parainfluenza virus.Methods Viruses were isolated by using chicken embryo and mutant lung fibroblast of human embryo(Z-HL-16C).The viruses isolated were assayed by hemagglutination test(HA),hemagglutination inhibition test (HAI)with several international and national influenza strains,vitus antigen slides of them were also assayed by specific cross test with McAb against 4 main respiratory track viruses.Results HA titer of two isolated strains was high,The results of HAI test...

Objective To isolate and identify parainfluenza virus.Methods Viruses were isolated by using chicken embryo and mutant lung fibroblast of human embryo(Z-HL-16C).The viruses isolated were assayed by hemagglutination test(HA),hemagglutination inhibition test (HAI)with several international and national influenza strains,vitus antigen slides of them were also assayed by specific cross test with McAb against 4 main respiratory track viruses.Results HA titer of two isolated strains was high,The results of HAI test were nagative when assayed with anti-sera of FluA and FluB virus,but strong fluorescence were obtained when antigen slides of two isolated strains were tested with PIV-2 McAb,pathological changes of cell fusion were obtained in Z-HL-16C.Conclusions The virus isolated were not influenza virus,and identified as PIV-2 by results of McAb fluorescent staining and pathologocal changes in Z-HL-16C.

目的 副流感病毒的分离、鉴定。方法 应用鸡胚和人胚肺成纤维细胞突变株分离病毒 ,用多株流感国际和国内代表株抗血清进行血凝抑制交互试验 ,用 4类主要呼吸道病毒单克隆抗体间接免疫荧光法做特异性交叉反应鉴定。结果 分离株在鸡胚培养中有较高的血凝效价 ,但与流感病毒甲、流感病毒乙均不发生血抑反应 ,而与副流感病毒2型单克隆抗体发生较强免疫荧光反应 ,在人胚肺成纤维细胞突变株培养出现明显细胞融合病变。结论 分离株虽具有高滴度血凝效价 ,但不是流感病毒 ,根据与副流感病毒 2型单克隆抗体可以发生较强反应和在人胚肺成纤维细胞上的特征性细胞病变 ,确认为副流感病毒 2型。

 
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