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肝细胞再生增强因子
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  “肝细胞再生增强因子”译为未确定词的双语例句
     To clone the fragment of human liver regeneration(hALR) gene and construct the eucaryote expression vector for hALR expression,we extract total RNA from 6-month old fetal liver. and amplified hALR gene fragment by RT-PCR technique,The PCR product was cloned into PET28a(+) vector and recombinants were identified by PCR and enzyme digestion.
     从6月龄流产的胎儿肝脏中提取总RNA,利用反转录-PCR(RT-PCR)技术扩增出人肝细胞再生增强因子hALR(Human augmenter of liver regeneration)基因片段,克隆于PET28a(+)载体,经进一步PCR及酶切鉴定,确定为此目的片段后进行序列分析,再将目的片段亚克隆于真核表达载体pcDNA3.1(+),用酶切方法鉴定重组子。
短句来源
     Effects of recombinant augmenter of liver regeneration protein,danshen and oxymatrine on rat fibroblasts
     重组肝细胞再生增强因子丹参及苦参对大鼠纤维细胞的作用
短句来源
     Construction and Expression of a Vector to Express ALR in Sheep Mammary Glands
     绵羊乳腺特异表达人肝细胞再生增强因子载体的构建及表达
短句来源
     separated one kind hot stability cell factor in 1994. Because it could promote liver cell multiplication, so it was named augmenter of liver regeneration (ALR).
     1994年Hagiya等人从初断乳大鼠的肝胞液中分离出一种能促进肝细胞增殖的一种热稳定性细胞因子,命名为肝细胞再生增强因子
短句来源
     We systemically studied the bioactivities of the hALR acquired by gene cloning method by establishing the big rat liver fibrosis model and small rat fat liver damage model. The work settle the fundamental for earlier using hALR in clinical to cure liver diseases.
     并通过建立大鼠肝纤维化模型和小鼠的脂肪肝损伤的模型,较系统的研究了基因克隆方法获得的人肝细胞再生增强因子基因的生物活性,为使hALR尽早应用于临床治疗肝病奠定了基础。
短句来源
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  相似匹配句对
     Herpes Simplex Virus Infection in Regenerating Mouse Liver
     单纯疱疹病毒在再生肝细胞中的增殖
短句来源
     atrophy and nodular hyperplasia of liver cells.
     肝细胞萎缩及结节状再生相伴。
短句来源
     Studies on the Mechanism of Shark Hepatic Regenerative Factor on Rat Liver Regeneration
     鲨鱼肝再生因子肝细胞再生的作用
短句来源
     regeneration and beneficial circulation.
     再生和循环。
短句来源
     PREPARATI0N AND BIOLOGICAL CHARACTERIZTION OF HEPATIC STIMULATOR SUBSTANCE FROM NEONATAL CATTLE LIVER
     牛肝细胞再生刺激因子的制备及其生物学特性
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  augmenter of liver regeneration (alr)
Augmenter of liver regeneration (ALR) may promote liver regeneration by reducing natural killer (NK) cell activity in human live
      
An augmenter of liver regeneration (ALR) inhibits liver natural killer cell activity in rats.
      


Human ALR gene and sheep BLG genes promoter sequences were amplified from human and sheep genomic DNA using PCR.The breast specific expression of ALR constructed by plasmid p7zf(+) was controlled by BLG promoter,and the non-specific expression of EGFP gene was controlled by CMV dual promoter system as genetic marker in the same vector.Sheep fetal fibroblast cell were transfected with p7zf-BAE by using LipofectAMINETM substance.Transgenic cells were identified by fluorescent microscope and PCR technique.The results...

Human ALR gene and sheep BLG genes promoter sequences were amplified from human and sheep genomic DNA using PCR.The breast specific expression of ALR constructed by plasmid p7zf(+) was controlled by BLG promoter,and the non-specific expression of EGFP gene was controlled by CMV dual promoter system as genetic marker in the same vector.Sheep fetal fibroblast cell were transfected with p7zf-BAE by using LipofectAMINETM substance.Transgenic cells were identified by fluorescent microscope and PCR technique.The results showed that the EGFP gene expressed independently in the SFFCs,and the BLG and ALR genes existed in the transgenic cells as well.

用PCR技术分别从人和绵羊基因组DNA中扩增人肝细胞再生增强因子(Humanaugmenterofliverregeneration,hALR)基因和绵羊β-乳球蛋白(sheepbeta-lactoglobulin,BLG)基因启动区序列,从pEGFP-C1质粒中扩增增强绿色荧光蛋白(EnhancedGreenfluorescenceprotein,EGFP)基因及其表达调控元件.由质粒p7zf(+)构建成ALR基因乳腺特异表达、EGFP基因非组织特异性表达载体.同时体外培养绵羊胎儿成纤维细胞(sheepfetalfibroblastcells,SFFCs),脂质体介导载体DNA转染SFFCs,激光共聚焦显微镜观察和PCR检测转基因细胞,结果表明,增强绿色荧光蛋白基因在SFFCs中表达,转基因细胞中可扩增出BLG、ALR、EGFP基因条带.

To clone the fragment of human liver regeneration(hALR) gene and construct the eucaryote expression vector for hALR expression,we extract total RNA from 6-month old fetal liver.and amplified hALR gene fragment by RT-PCR technique,The PCR product was cloned into PET28a(+) vector and recombinants were identified by PCR and enzyme digestion.The analyzed gene sequence showed that it has the basic sequence with the one in Genebank.The target gene was subcloned into eucaryote expression vector pcDNA3.1(+) and recombinants...

To clone the fragment of human liver regeneration(hALR) gene and construct the eucaryote expression vector for hALR expression,we extract total RNA from 6-month old fetal liver.and amplified hALR gene fragment by RT-PCR technique,The PCR product was cloned into PET28a(+) vector and recombinants were identified by PCR and enzyme digestion.The analyzed gene sequence showed that it has the basic sequence with the one in Genebank.The target gene was subcloned into eucaryote expression vector pcDNA3.1(+) and recombinants were identified by enzyme digestion.The successfully construction of the eucaryote expression vector provide the foundation of hALR eucaryote expression.

从6月龄流产的胎儿肝脏中提取总RNA,利用反转录-PCR(RT-PCR)技术扩增出人肝细胞再生增强因子hALR(Human augmenter of liver regeneration)基因片段,克隆于PET28a(+)载体,经进一步PCR及酶切鉴定,确定为此目的片段后进行序列分析,再将目的片段亚克隆于真核表达载体pcDNA3.1(+),用酶切方法鉴定重组子。采用RT-PCR技术成功地获得了hALR基因片段。序列分析表明,克隆的hALR基因与Genbank报道的人ALR核苷酸序列基本一致。完成了真核表达载体的构建,为hALR基因的真核表达奠定了基础。

Human ALR gene were amplified from human genomic DNA using PCR and inserted into the MDS of pEGFP-C1 plasmid to construct ALR and EGFP co-expression vector pEGFP/ALR.It was transferred into the Hela cells and selected by G418.ALR gene of transgenic cells was tested by PCR and SDS-PAGE and EGFP were observed with the confocal laser scanning microscope.The result showed that both PCR and enzyme digestion analysis confirmed constructed plasmids were correct,the ALR gene were detected in these positive cells.SDS-PAGE...

Human ALR gene were amplified from human genomic DNA using PCR and inserted into the MDS of pEGFP-C1 plasmid to construct ALR and EGFP co-expression vector pEGFP/ALR.It was transferred into the Hela cells and selected by G418.ALR gene of transgenic cells was tested by PCR and SDS-PAGE and EGFP were observed with the confocal laser scanning microscope.The result showed that both PCR and enzyme digestion analysis confirmed constructed plasmids were correct,the ALR gene were detected in these positive cells.SDS-PAGE results indicated that there was specific 57KD protein band in the positive cells,which matches the expression of EGFP as well.Therefore,EGFP can be used as a marker for target gene in the screening of transgenic cells.

从人血液中提取总DNA,利用PCR技术扩增人肝细胞再生增生因子基因,将其插入表达载体pEGFP-C1的多克隆位点中,构建增强绿色荧光蛋白(enhanced green fluorescence protein gene,EGFP)和人肝细胞再生增强因子(human augmenter of liver regeneration gene,ALR)融合基因表达载体pEGFP/ALR,并将其转染Hela细胞系,用含G 418的DMEM/F12培养液筛选转基因细胞,然后利用PCR和聚丙烯酰胺凝胶电泳检测转基因细胞中ALR基因的存在及其表达,用荧光显微镜检测EGFP基因的表达.结果显示:得到了构建正确的EGFP和ALR融合基因表达载体;在转基因细胞中,PCR扩增得到1.7 Kb的ALR条带,蛋白电泳得到57 KD大小条带,与ALR和EGFP融合蛋白大小相符;荧光显微镜下观察到发绿色荧光的Hela细胞.在转基因细胞中,EGFP和ALR同时存在并表达,绿色荧光蛋白可作为报告基因指示目的基因的表达,从而简化了目的基因繁琐的检测手段.

 
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