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   肝细胞再生增强因子 在 生物学 分类中 的翻译结果: 查询用时:0.058秒
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  “肝细胞再生增强因子”译为未确定词的双语例句
    Construction and Expression of a Vector to Express ALR in Sheep Mammary Glands
    绵羊乳腺特异表达人肝细胞再生增强因子载体的构建及表达
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    Human ALR gene and sheep BLG genes promoter sequences were amplified from human and sheep genomic DNA using PCR. The breast specific expression of ALR constructed by plasmid p7zf(+) was controlled by BLG promoter,and the non-specific expression of EGFP gene was controlled by CMV dual promoter system as genetic marker in the same vector.
    用PCR技术分别从人和绵羊基因组DNA中扩增人肝细胞再生增强因子(Humanaugmenterofliverregeneration,hALR)基因和绵羊β-乳球蛋白(sheepbeta-lactoglobulin,BLG)基因启动区序列,从pEGFP-C1质粒中扩增增强绿色荧光蛋白(EnhancedGreenfluorescenceprotein,EGFP)基因及其表达调控元件.
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Human ALR gene and sheep BLG genes promoter sequences were amplified from human and sheep genomic DNA using PCR.The breast specific expression of ALR constructed by plasmid p7zf(+) was controlled by BLG promoter,and the non-specific expression of EGFP gene was controlled by CMV dual promoter system as genetic marker in the same vector.Sheep fetal fibroblast cell were transfected with p7zf-BAE by using LipofectAMINETM substance.Transgenic cells were identified by fluorescent microscope and PCR technique.The results...

Human ALR gene and sheep BLG genes promoter sequences were amplified from human and sheep genomic DNA using PCR.The breast specific expression of ALR constructed by plasmid p7zf(+) was controlled by BLG promoter,and the non-specific expression of EGFP gene was controlled by CMV dual promoter system as genetic marker in the same vector.Sheep fetal fibroblast cell were transfected with p7zf-BAE by using LipofectAMINETM substance.Transgenic cells were identified by fluorescent microscope and PCR technique.The results showed that the EGFP gene expressed independently in the SFFCs,and the BLG and ALR genes existed in the transgenic cells as well.

用PCR技术分别从人和绵羊基因组DNA中扩增人肝细胞再生增强因子(Humanaugmenterofliverregeneration,hALR)基因和绵羊β-乳球蛋白(sheepbeta-lactoglobulin,BLG)基因启动区序列,从pEGFP-C1质粒中扩增增强绿色荧光蛋白(EnhancedGreenfluorescenceprotein,EGFP)基因及其表达调控元件.由质粒p7zf(+)构建成ALR基因乳腺特异表达、EGFP基因非组织特异性表达载体.同时体外培养绵羊胎儿成纤维细胞(sheepfetalfibroblastcells,SFFCs),脂质体介导载体DNA转染SFFCs,激光共聚焦显微镜观察和PCR检测转基因细胞,结果表明,增强绿色荧光蛋白基因在SFFCs中表达,转基因细胞中可扩增出BLG、ALR、EGFP基因条带.

Human ALR gene were amplified from human genomic DNA using PCR and inserted into the MDS of pEGFP-C1 plasmid to construct ALR and EGFP co-expression vector pEGFP/ALR.It was transferred into the Hela cells and selected by G418.ALR gene of transgenic cells was tested by PCR and SDS-PAGE and EGFP were observed with the confocal laser scanning microscope.The result showed that both PCR and enzyme digestion analysis confirmed constructed plasmids were correct,the ALR gene were detected in these positive cells.SDS-PAGE...

Human ALR gene were amplified from human genomic DNA using PCR and inserted into the MDS of pEGFP-C1 plasmid to construct ALR and EGFP co-expression vector pEGFP/ALR.It was transferred into the Hela cells and selected by G418.ALR gene of transgenic cells was tested by PCR and SDS-PAGE and EGFP were observed with the confocal laser scanning microscope.The result showed that both PCR and enzyme digestion analysis confirmed constructed plasmids were correct,the ALR gene were detected in these positive cells.SDS-PAGE results indicated that there was specific 57KD protein band in the positive cells,which matches the expression of EGFP as well.Therefore,EGFP can be used as a marker for target gene in the screening of transgenic cells.

从人血液中提取总DNA,利用PCR技术扩增人肝细胞再生增生因子基因,将其插入表达载体pEGFP-C1的多克隆位点中,构建增强绿色荧光蛋白(enhanced green fluorescence protein gene,EGFP)和人肝细胞再生增强因子(human augmenter of liver regeneration gene,ALR)融合基因表达载体pEGFP/ALR,并将其转染Hela细胞系,用含G 418的DMEM/F12培养液筛选转基因细胞,然后利用PCR和聚丙烯酰胺凝胶电泳检测转基因细胞中ALR基因的存在及其表达,用荧光显微镜检测EGFP基因的表达.结果显示:得到了构建正确的EGFP和ALR融合基因表达载体;在转基因细胞中,PCR扩增得到1.7 Kb的ALR条带,蛋白电泳得到57 KD大小条带,与ALR和EGFP融合蛋白大小相符;荧光显微镜下观察到发绿色荧光的Hela细胞.在转基因细胞中,EGFP和ALR同时存在并表达,绿色荧光蛋白可作为报告基因指示目的基因的表达,从而简化了目的基因繁琐的检测手段.

 
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