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      单克隆培养
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  monoclone culture
     Study of ~(60)Co-γ irradiation and monoclone culture in thallus of Porphyra haitanensis
     ~(60)Co-γ射线辐照坛紫菜叶状体及单克隆培养的研究
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     This article reports the study of cleavage and differentiation and monoclone culture of the somatic cells which were enzymic-degraded from thallus of Porphyra haitanensis after inducing mutation by ()~(60)Co-γ ray irradiation.
     本文报道了坛紫菜叶状体经60Coγ射线辐照后,用酶解方法获得离体细胞,观察了体细胞的分裂分化及进行单克隆培养叶状体.
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  “单克隆培养”译为未确定词的双语例句
     In laboratory,SPC-A-1 cell line with highly expression of IGF1R was obtained by monoclonal techniques and distributed into B,C,D,E,F,G and H groups.
     实验部分:对SPC-A-1细胞株进行单克隆培养,筛选出IGF1R高表达细胞株,并分为B、C、D、E、F、G、H组。
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     1. Fast propagation of gaznetophyte clones:(1).
     6天的生长之用(单克隆培养密度1-10g/L)。
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     Methods Interleukin-18-PE38 fusion gene was cleaved from plasmid PRKL459k-IL-18-PE38 by restriction enzyme digestion,then linked with vectors PsecTag2B and transformed into competence bacteria, positive clones were selected and confirmed by restrictive enzyme(EcoRI) digestion assay.
     方法 经限制性内切酶双酶切从质粒PRKL4 5 9K- IL18- PE38中获取IL- 18- PE38融合基因,将其与真核表达载体Psec Tag2 B连接,转化感受态菌,挑取单克隆培养并提取质粒,Eco R 单酶切鉴定。
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     Human bone mesenchymal stem cells(MSCs) were isolated,cultured and proliferated by Percoll gradient centrifugation,adherence to plastic flask and monoclonal culture.
     利用Percoll梯度分离、贴壁筛选法及单克隆培养法分离、培养人骨髓间充质干细胞(mesenchymal stem cells,MSCs);
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     Rat skeletal myoblasts were selected with continuous adherence method for monoclonal cultivation by inoculating into a 96-pore plate.
     以连续贴壁法筛选大鼠骨骼肌成肌细胞,接种于96孔板进行单克隆培养
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  相似匹配句对
     Hybridoma Cell Culture Producing Monoclonal Antibody
     培养杂交瘤细胞制备单克隆抗体的工艺过程
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     Continuous Perfusion Culture Hybridoma Cells for Production of Monoclonal Antibody
     连续灌流培养杂交瘤细胞生产单克隆抗体
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     On the Cultivation of Esthetic Judgment
     审美能力的培养
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     Then how?
     如何培养?
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  colony culture
For the suspension and colony culture growth of the cells, the IC50 concentrations of 6HT were 500±46 and 350±37 nM, while those of taxol were 3.2±0.2 and 2.8±0.5 nM, respectively.
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We found the agar colony culture method to be most suitable and methodologically most advantageous.
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Based on the results of inverted colony culture, it would appear that some isolates of Trichoderma produce inhibitory volatile compounds.
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Colonies were then transferred to colony culture medium (0.4% Gelrite-solidified protoplast culture medium) and maintained by subculturing at 4-week intervals to form embryogenic calluses.
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The morphology of differentiating mouse astrocytes in colony culture
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         A toxic dinoflagellate Alexandrium tamarense collected in 1991, fromDapeng Bay in the South China Sea was studied to assess its sensitiveness to differentUV radative doses (0, 95, 190, 380, 760 and 1520J /m2) under four different pairsof culture condtions, i. e., continulng light for 24h to continulng dark for 24h, different UV radiative intensities of 5.9W /m2 and 9.5W /m2, lack of nitrogen to half nitrogen concentration (6-17mg /L) and lack of phosphorus to half phosphorusconcentration (646μg /L).The resu...
            对1991年分离自南海大鹏湾海域的塔玛亚历山大藻单克隆培养林在不同光照条件、辐射强度(9.5,5.9W/m2)(辐射剂量为:95,190,380,760,1520J/m2)和营养盐(N,P)浓度下,进行紫外辐射敏感性的研究。结果表明,紫外辐射可以导致细胞变形、增大和死亡。不论在什么条件下,低剂量的紫外辐射(95J/m2)就能使该藻的存活率和生长率大幅下降。此后,随着辐射剂量的增加,虽然存活率和生长率继续下降,但下降幅度渐缓。充足的营养盐和辐射处理后的连续光照对藻群的恢复有益,但在缺氮和缺磷条件下培养的藻群,可能由于生理活性减弱而导致对紫外辐射敏感性的迟钝。辐射强度对该藻的紫外辐射敏感性的影响十分显著,而且与辐射剂量具有明显的双重效应。
文摘来源
         The schistosomula of Schistosoma japonicum were transformed from cercariae released from the snails by syringe method and incubated in the RPMI 1640 medium, which contained 10% rabbit serum and maintained in monoclonal culture plate. Different densities of crystal toxin from the Bacillus thuringiensis (Strain YBT 1955), were added separately in the hole of plate and incubated at 37℃, 5% CO 2. The activity of the schistosomula became low and some of the worms died after being incubated for 12 h as obs...
            将感染日本血吸虫的钉螺常规逸蚴,经注射器推压机械断尾获得的童虫,加入内含10%兔血清的RPMI-1640培养液后,置于单克隆培养板内,以苏云金芽胞杆菌(菌株YBT-1955)制得的伴胞晶体毒素,以不同的浓度加入以上各孔中于37℃,5%CO2培养箱内培养培养12h后发现童虫活动力减弱,有些已经死亡;24h后,随着加入伴胞晶体毒素浓度的增加,童虫死亡率也相应增加,呈正相关(r=0.897)。经毒素蛋白含量为40mg/L处理的童虫,24h后死亡率为100%,LD50为15.95μg(1mL)。
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         Paramecium caudatum(P.c.)cultured by clone was treated with He—Ne laser.The results showed irradiation promoted the division and reproduction of P.c. when the dosages of laser were between 50.9 J/cm 2 and 101.9 J/cm 2 .The most proper dosage was 101.9 J/cm 2 under the condition of this experiment. The speed was accelerated or,at least,no disadvantage effect on division and reproduction of P.c.under very intensive and very wide range of dosages from 25.5J/cm 2 to 152.8J/cm 2.The results indicated t...
            采用He—Ne激光辐照经单克隆培养的尾草履虫(Parameciumcauatum)无性繁殖系,观察并分析激光对尾草履虫分裂繁殖产生的影响。结果表明,在本实验条件下,当辐照的能量密度处于50.9—101.9(J/cm2)范围内,激光对尾草履虫的分裂繁殖产生促进作用。当辐照的能量密度为101.9J/cm2时,草履虫分裂繁殖的个体平均数达最大值。草履虫对激光辐照有很大的耐受范围和很强的耐受能力。本实验采用的辐照剂量范围宽(从25.5J/cm2至152.8J/cm2),辐照剂量大(最高达152.8J/cm2),但对草履虫分裂繁殖表现为起促进作用或无不利影响。这一结果说明,最原始、最低等的单细胞动物对激光有强的耐受性;同时,从对光的反应方面,为原生动物的生态研究提供数据资料
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