This article reports the study of cleavage and differentiation and monoclone culture of the somatic cells which were enzymic-degraded from thallus of Porphyra haitanensis after inducing mutation by ()~(60)Co-γ ray irradiation.
Methods Interleukin-18-PE38 fusion gene was cleaved from plasmid PRKL459k-IL-18-PE38 by restriction enzyme digestion,then linked with vectors PsecTag2B and transformed into competence bacteria, positive clones were selected and confirmed by restrictive enzyme(EcoRI) digestion assay.
Human bone mesenchymal stem cells(MSCs) were isolated,cultured and proliferated by Percoll gradient centrifugation,adherence to plastic flask and monoclonal culture.
For the suspension and colony culture growth of the cells, the IC50 concentrations of 6HT were 500±46 and 350±37 nM, while those of taxol were 3.2±0.2 and 2.8±0.5 nM, respectively.
Colonies were then transferred to colony culture medium (0.4% Gelrite-solidified protoplast culture medium) and maintained by subculturing at 4-week intervals to form embryogenic calluses.
A toxic dinoflagellate Alexandrium tamarense collected in 1991, fromDapeng Bay in the South China Sea was studied to assess its sensitiveness to differentUV radative doses (0, 95, 190, 380, 760 and 1520J /m2) under four different pairsof culture condtions, i. e., continulng light for 24h to continulng dark for 24h, different UV radiative intensities of 5.9W /m2 and 9.5W /m2, lack of nitrogen to half nitrogen concentration (6-17mg /L) and lack of phosphorus to half phosphorusconcentration (646μg /L).The resu...
The schistosomula of Schistosoma japonicum were transformed from cercariae released from the snails by syringe method and incubated in the RPMI 1640 medium, which contained 10% rabbit serum and maintained in monoclonal culture plate. Different densities of crystal toxin from the Bacillus thuringiensis (Strain YBT 1955), were added separately in the hole of plate and incubated at 37℃, 5% CO 2. The activity of the schistosomula became low and some of the worms died after being incubated for 12 h as obs...
Paramecium caudatum(P.c.)cultured by clone was treated with He—Ne laser.The results showed irradiation promoted the division and reproduction of P.c. when the dosages of laser were between 50.9 J/cm 2 and 101.9 J/cm 2 .The most proper dosage was 101.9 J/cm 2 under the condition of this experiment. The speed was accelerated or,at least,no disadvantage effect on division and reproduction of P.c.under very intensive and very wide range of dosages from 25.5J/cm 2 to 152.8J/cm 2.The results indicated t...