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肾近端小管细胞
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  “肾近端小管细胞”译为未确定词的双语例句
     Expression of Megalin and Cubilin in Cultured Proximal Tubule Cells of Opossum Kidney
     受体Megalin和Cubilin在培养的Opossum肾近端小管细胞的表达
短句来源
     (2) Fabrication of RAD with hPTCs.
     (2) 构建RAD用培养的人肾近端小管细胞构建RAD;
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     Effect of high concentration of glucose on the expression of TGF-β mRNA and its relationship with the effect of angiotensin Ⅱ in human proximal tubular cells
     高糖对人肾近端小管细胞TGF-β mRNA表达的影响
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     Methods (1) Primary culture, subculture and identification of human renal proximal tubule cells (PTC).
     材料和方法 (1) 细胞培养 原代培养人肾近端小管细胞,传代并鉴定;
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     Therefore nephrotoxicity is best studied in these particular cells. The OK and LLC-PK1 cells are generally used, however, the OK cell can not express the marker enzyme of proximal tubular cells -AP and y -GT. LLC-PK1 cell lacks of the G-6-P and hexokinase and can not glyconeogenesis.
     目前国内外常用的研究模型是OK和LLC-PK1细胞,然而OK细胞不表达肾近端小管细胞标志酶——碱性磷酸酶和γ-谷氨酰转肽酶,而LLC-PK1细胞因缺乏葡萄糖-6-磷酸酶和己糖激酶,无法进行糖原异生。
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  相似匹配句对
     THE CULTURE OF PROXIMAL TUBULE CELLS OF RABBIT KIDNEY
     兔管细胞的培养
短句来源
     Expression of Megalin and Cubilin in Cultured Proximal Tubule Cells of Opossum Kidney
     受体Megalin和Cubilin在培养的Opossum管细胞的表达
短句来源
     Meth-ods:Primary culture of rabbit proximal tubular cells was established.
     方法 :在体外建立原代兔管细胞培养。
短句来源
     (2) Fabrication of RAD with hPTCs.
     (2) 构建RAD用培养的人管细胞构建RAD;
短句来源
     Protective Effects of VE on Renal Proximal Tubular Cells of Mice With Chronical Cadmium Poisoning
     维生素E对慢性镉染毒管细胞损伤的保护作用
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  renal proximal tubular cell
How big is the electrochemical potential difference of Na+ across rat renal proximal tubular cell membranes in vivo
      
We have used somatic cell genetic techniques to probe the role of PKA in controlling morphology and behavior of a renal epithelial cell line, LLC-PK1, which acquires many properties characteristic of the renal proximal tubular cell.
      
Receptors for both GH and IGF I are present in the basolateral membrane of the renal proximal tubular cell.
      
Additionally, rapamycin has been shown to have a direct effect on renal proximal tubular cell function.
      
Model of a renal proximal tubular cell with sulfate transport systems.
      
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It is a new approach to investigate the ultrastructure, function and metabolism of each segment of uriniferous tubule using dual environment culture chambers (DECC). In the present study the DECC culture of proximal tubule cells was undertaken with millcell-HA culture insert in the mixed medium of Ham's F12 and Dulbecco's modified Eagle's medium.A monolayer of proximal tubule cells was obtained in the medium mixed by constant bubling of oxygen at 3 weeks after seeding. Transmission electron microscopy revealed...

It is a new approach to investigate the ultrastructure, function and metabolism of each segment of uriniferous tubule using dual environment culture chambers (DECC). In the present study the DECC culture of proximal tubule cells was undertaken with millcell-HA culture insert in the mixed medium of Ham's F12 and Dulbecco's modified Eagle's medium.A monolayer of proximal tubule cells was obtained in the medium mixed by constant bubling of oxygen at 3 weeks after seeding. Transmission electron microscopy revealed the cuboidal cells with microvilli, abundant mitochondria, vacuoles, lysosomes, and irregular plasma membrane infoldings. Also, the monolayer exhibited active metabolism from the release of ~(14)C-I-carbon dioxide and absorption ability of ~(14)C-I-glucose across the cultured monolayer. In addition, the culture procedure has been introduced in detail.

本文将percoll分离的兔肾近端小管细胞,应用微细胞培养插盘,在Ham F 12和Dulbecco's modified Eagle混合培养液中进行双室培养。应用充氧的培养液培养3周后,获得一层完整的近端小管细胞。透射电镜观察表明,细胞具有近端小管细胞的特点,如微绒毛的出现、较丰富的线粒体、吞饮小泡、溶酶体以及不规则的质膜内褶。~(14)C标记的葡萄糖吸收试验,以及“~(14)CO_2释放的测定,说明培养出的一层近端小管细胞重吸收功能完好,代谢旺盛。本文并对该方法进行了较详细的介绍和分析。

AIM: To study the mechanism of cisplatin interaction with DNA, and the attenuating effects of 6,7-dimethoxycoumarin ( DMOC ) on crosslink. METHODS: Primary cultured rabbit kidney proximal tubular cells (PTC) were established. DNA interstrand crosslink was assayed with ethidium bromide binding and DNA-protein crosslink with 125I-postlabelling. PTC were incubated with cisplatin for 24 h. DMOC was preincubated with PTC for 24 h, and cisplatin (26 μmol·L-1) was added into culture and incubated for another 24 h....

AIM: To study the mechanism of cisplatin interaction with DNA, and the attenuating effects of 6,7-dimethoxycoumarin ( DMOC ) on crosslink. METHODS: Primary cultured rabbit kidney proximal tubular cells (PTC) were established. DNA interstrand crosslink was assayed with ethidium bromide binding and DNA-protein crosslink with 125I-postlabelling. PTC were incubated with cisplatin for 24 h. DMOC was preincubated with PTC for 24 h, and cisplatin (26 μmol·L-1) was added into culture and incubated for another 24 h. RESULTS: Cisplatin induced formation of DNA interstrand crosslink (13, 26, 52, and 78 μmol·L-1) and DNA-protein crosslink (26, 52, and 78μmol·L-1) (P<0.01). DNA interstrand crosslink in DMOC (0.4, 4, and 8 mg · L-1) and DNA-protein crosslink in DMOC (4,8 mg·L-1) were less than those in cisplatin group (26 μmol·L-1), respectively ( P < 0.01). CONCLUSION: The mechanisms of cisplatin interaction with DNA in PTC were DNA interstrand crosslink and DNA-protein crosslink, and DMOC attenuated these effects in vitro.

目的:研究顺铂与肾近端小管DNA的作用机制,和茵陈素的干预作用,方法:原代培养兔肾近端小管细胞(PTC).溴乙锭荧光测DNA链间交联,~(125)Ⅰ标记测DNA-蛋白交联,顺铂与PTC保温24h.茵陈素与PTC提前24 h保温后,加入顺铂26μmol·L~(-1)再保温24 h,结果:顺铂13到78 μmol·L~(-1)和26到78 μmol·L~(-1)可使PTC形成DNA链间交联和DNA-蛋白交联,茵陈素(0.4,4,8 mg·L~(-1))和(4,8 mg·L~(-1))组,DNA链间交联和DNA-蛋白交联分别低于顺铂(26μmol·L~(-1)),结论:顺铂导致肾近端小管形成DNA链间交联和DNA-蛋白交联,茵陈素减弱这两种作用。

AIM: To study protective effects of Panax notoginseng saponins ( PnS ) against cisplatin-nephrotoxicity. METHODS: Cisplatin-induced nephrotoxicity in mice in vivo, and primary culture of rabbit proximal tubular cells (PTC) in vitro were established. Blood urea nitrogen, serum creatinine, cell viability, DNA interstrand crosslink, DNA-protein cross-link, and cytosolic free [Ca2+]i were assayed with diacetyl monoxime, alkaline picrate, trypan blue, ethidium bromide binding, 125I-postla-belling, and Fur 2-AM, respectively....

AIM: To study protective effects of Panax notoginseng saponins ( PnS ) against cisplatin-nephrotoxicity. METHODS: Cisplatin-induced nephrotoxicity in mice in vivo, and primary culture of rabbit proximal tubular cells (PTC) in vitro were established. Blood urea nitrogen, serum creatinine, cell viability, DNA interstrand crosslink, DNA-protein cross-link, and cytosolic free [Ca2+]i were assayed with diacetyl monoxime, alkaline picrate, trypan blue, ethidium bromide binding, 125I-postla-belling, and Fur 2-AM, respectively. RESULTS: With pretreatment for 2 d in mice, PnS 100 and 200 mg·kg-1·d-1 suppressed cisplatin-induced high blood urea nitrogen level to 83 % and 31 % , and serum creatinine level to 86 % and 42 % , respectively (P < 0.01). Preincubated with PTC for 24 h, PnS 10 and 100 mg· L-1 inhibited cisplatin-induced .decrease of cell viability from 78 % to 81 % (P <0.05) and 89 % (P < 0.01), respectively. PnS 10 and 100 mg·L-1 suppressed formations of DNA interstrand cross-link to 47 % and 40 %, DNA-protein interstrand cross-link to 77 % and 42 %, and cytosolic free [Ca2+ ]i overload in PTC to 70 % and 63 % , respectively. ( P < 0.01). CONCLUSION: PnS was a prophylactic for cisplatin-induced nephrotoxicity, and mechanisms were relevant to the effects that PnS reduced cisplatin-induced cytosolic free [Ca2+ ]i overload, and formations of DNA interstrand cross-link and DNA-protein cross-link.

目的:研究三七皂苷(PnS)对顺铂肾毒性的防护作用。方法:采用小鼠和原代兔肾近端小管细胞培养(FTC)建立体内外顺铂肾毒性模型。用双乙酰、苦味酸、溴乙锭、台酚蓝、~(125)碘标记和Fura 2-AM方法分别测血尿素氮、血清肌酐、细胞存活率、DNA链间交联、DNA-蛋白交联和细胞内游离钙离子。结果:预先2d给PnS(100,200mg·kg~(-1)·d~(-1))使顺铂导致的小鼠血尿素氮下降到83%和31%,血清肌酐下降到86%和42%(P<0.01)。提前24h PnS(10,100mg/L)与PTC孵育,细胞存活率从顺铂组的78%提高到81%和89%,DNA链间交联下降到47%和40%,DNA-蛋白交联下降到77%和42%,细胞内游离钙下降到70%和63%(P<0.01)。结论:PnS可预防顺铂的肾毒性,其机制是降低顺铂导致的DNA链间交联、DNA-蛋白交联和钙离子超载。

 
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