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宫颈癌细胞
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cervical carcinoma cell
This article discusses the characteristics of the drug-resistant human cervical carcinoma cell line HeLa/MMC and investigates reversal effect of SDZ-PSC833(PSC833) and Verapamil (Ver) upon HeLa/MMC.
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Objective: To investigate the regulatory effect of curcumin on proliferation and apoptosis in human cervical carcinoma cell line HeLa in vitro.
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Methods: Human cervical carcinoma cell line Hela was cultured in vitro.
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Cervical carcinoma cell lines containing from 1-3 to 600 copies of the HPV genome were used as positive controls and were scored positive in all assays by both Southern blotting and GP-PCR.
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Cells from the ME-180 cervical carcinoma cell line were used as target cells.
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We investigated here the expression pattern of AP-2α, AP-2β, and AP-2γ, as well as that of the cellular AP-2 target gene c-erbB-2, in a series of cervical cancer cell lines.
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We found that, with the exception of HPV-negative C33A cells, all investigated cervical cancer cell lines expressed all three AP-2 family members, although at varying levels.
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Three of the five cell lines (ovarian cancer cell line BG-1, cervical cancer cell line ME-180, and melanoma cell line SK-MEL 28) were sensitive to both IFNs.Cervical cancer cell line CaSki was sensitive to IFN-α2b but resistant to IFN-γ.
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To investigate its in vitro activity toward cervical cancer, we exposed six cervical cancer cell lines to gemcitabine.
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Six of 10 ovarian cancer cell lines showed positive staining, while 3 of 5 cervical cancer cell lines were positive.
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Cervical cancer cells contain detectable levels of functional p53 protein despite of the expression of the HPV E6 protein, which can induce p53 degradation.
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These results indicate that p53 mutations are rare events in recurrent cervical carcinomas, and that somatic mutations of p53 do not provide cervical cancer cells with a selective growth advantage for recurrence.
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These results indicate that, although a rare event, loss of AP-2 expression occurs in cervical cancer cells.
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HPV DNA was detected in 16 (8.5%) of the 187 specimens tested, although in a low copy number compared with SiHa cervical cancer cells used as control.
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Conclusions: We were able to reprogramme the target specificity of recombinant Cl96 and na?ve CTLs resulting in efficient cytolysis of CD44v7/8-positive cervical cancer cells.
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Transcriptional inhibition of the human papilloma virus reactivates tumor suppressor p53 in cervical carcinoma cells
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Activation of p53 in cervical carcinoma cells was accompanied by induction of p53-dependent CDKN1 (p21), inhibition of cell proliferation, and induction of apoptosis.
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McCoy cells (mouse fibroblasts), HeLa 229 (derived from human cervical carcinoma cells) and BHK-21 cells (baby hamster kidney cells) are the cell types regularly used for the culture ofC.
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Reversal efficacy of SDZ-PSC833 and verapamil on drug-resistant human cervical carcinoma cells line
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Membrane oxidative damage and apoptosis in cervical carcinoma cells of patients after radiation therapy
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