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优势角
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     Realizability of Diagonal Diminance
     对优势的可实现性
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     angle.
    
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     ACHIEVING DIAGONAL DOMINANCE
     对优势化的实現
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     A New Type of Angle Acceleration Sensor
     加速度传感器
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     The Superiority of Literal Translation
     直译的优势
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The purpose of this study was to develop a quantitative and objective method for evaluating neurological deficits in mice with focal cerebral ischemia. After middle cerebral artery occlusion (MCAO) , the neurological deficits were evaluated 24 h later. We measured the mean angles, dominant angles and turns in a hanged test in which the mice were slicked on the wall, and the holding angles in an inclined plane test as well, Then we determined the cerebral infarct volumes, neuron density in hippocampus, cortex...

The purpose of this study was to develop a quantitative and objective method for evaluating neurological deficits in mice with focal cerebral ischemia. After middle cerebral artery occlusion (MCAO) , the neurological deficits were evaluated 24 h later. We measured the mean angles, dominant angles and turns in a hanged test in which the mice were slicked on the wall, and the holding angles in an inclined plane test as well, Then we determined the cerebral infarct volumes, neuron density in hippocampus, cortex and subcorti-cal areas 24 h after MCAO. The correlations among infarct volume, neuron density and neurological deficits were analyzed. We also compared the quantitative method with two typical complex methods of behavioral assessment. The effect of pranlukast, 4-oxo-8-[p-(4-phenylbutyloxy) benzoylamino]-2-(tetrazol-5-yl)-4H-1-benzopyran hemihydrate (ONO-1078) , a neuroprotective agent, on ischemic injury was observed using this method. We found that the variables measured by both quantitative and typical behavioral methods significantly changed in the ischemic mice, and correlated with the infarct volumes and neuron densities. The quantitative variables well correlated with those of typical behavioral assessment, too. ONO-1078 inhibited ischemic injury and reduced the total scores of quantitative assessment. Thus, the quantitative method we developed is useful in evaluating neurological deficits of focal cerebral ischemia with the advantages of objectivity, quantification, simplicity and non-invasion, and can be used in the evaluation of neuroprotective effects of drugs.

本文旨在建立一种客观评价小鼠局灶性脑缺血神经症状的定量方法。在大脑中动脉阻塞诱导局灶性脑缺血后24 h,采用悬挂试验分别测定转动的平均角和优势角以及转动次数,并用爬板试验测定小鼠攀爬角度;分析定量测定指标与脑梗死体积、神经元密度的相关性,并与经典的行为学评价方法比较。还以此法观察抗脑缺血药pranlukast,4-氧-8-[对-(4-苯丁氧基)苯甲酰氨基]-2-(5-四氮基)4H-1-苯并吡喃半水化合物(ONO-1078)的作用。结果显示,脑缺血小鼠各项指标均有显著改变,定量评价总分与脑梗死体积、神经元密度减少密切相关,与经典行为学评分之间也密切相关。ONO-1078可显著抑制缺血损伤,并降低定量评价总分。因此,本方法可反映局灶性脑缺血神经症状变化,具有客观、定量、操作简便、无损伤的优点,并能用于评价抗脑缺血药物的作用。

AIM: To observe time course of neurological deficits after focal cerebral ischemia in mice, and to confirm the quantitative and objective method we developed for evaluating neurological deficits. METHODS: Focal cerebral ischemia was induced by middle cerebral artery occlusion. The neurological deficits were assessed 6 h, 12 h, 24 h, or 1-7 d after ischemia. We measured the mean angles, dominant angles and turns by a computer-assisted video tracking technique in a hanged test, and the holding angles in an inclined...

AIM: To observe time course of neurological deficits after focal cerebral ischemia in mice, and to confirm the quantitative and objective method we developed for evaluating neurological deficits. METHODS: Focal cerebral ischemia was induced by middle cerebral artery occlusion. The neurological deficits were assessed 6 h, 12 h, 24 h, or 1-7 d after ischemia. We measured the mean angles, dominant angles and turns by a computer-assisted video tracking technique in a hanged test, and the holding angles in an inclined plane test. We also analyzed relations between quantitative behavioral assessment and other behavioral assessments, brain infarct volumes, or neuron densities in different brain regions. RESULTS: The behavioral variables measured by the quantitative assessment significantly changed in ischemic mice, similar to those by the complex behavioral assessment, and the total quantitative scores at 6 h after ischemia was higher than those at 12 h and 24 h. During 1-7 d after ischemia, the total quantitative scores had a lasting increase and a tendency of recovery from day 3, the mean angle and relative turn scores markedly increased at day 1, and the holding angle scores persistently increased throughout 7 days. However, the typical neurological scores constantly increased without recovery. Close relations existed between quantitative variables and those of other behavioral assessments, infarct volumes, or neuron densities in different brain regions. Among the quantitative variables, the holding angle was most stable. CONCLUSION: Neurological deficits were stably measured during 12 h to 7 days and recovered gradually in focal cerebral ischemic mice. The quantitative behavioral assessment is useful for getting objective and quantitative data, and holding angle seems to be the simplest and most stable variable for researchers to use in other laboratories.

目的 :确定我们建立的局灶性脑缺血神经症状定量评价方法的实用性。方法 :大脑中动脉阻塞诱导小鼠局灶性脑缺血后 6、12、2 4h以及 1- 7d ,在悬挂试验以计算机视频跟踪技术测定平均角、优势角和转动次数 ,在爬板试验测定爬板角度 ;分析定量评价指标与其他行为学评价方法以及脑梗死体积和神经元密度的相关性。结果 :脑缺血后各项定量评价指标均有显著改变 ,与行为学综合评价的结果相似 ,6h的定量评价总分高于 12、2 4h。缺血后 1- 7d内 ,定量评价总分持续增高 ,但在 3d后有下降 ;平均角与相对转动分值在 1d明显增高 ;爬板角度分值持续增高 ;经典的神经症状评分则持续增高而无下降。定量评价总分与其他行为学评价以及脑梗死体积和各脑区神经元密度密切相关 ,其中爬板角度最稳定。结论 :小鼠局灶性脑缺血后 12h至 7d的神经症状稳定 ,并可逐渐恢复 ;定量评价方法可提供客观、定量结果 ,与其他行为学方法及形态学密切相关 ,其中爬板角度最简便、稳定 ,可以推广试用。

Based on the engineering geologic section model and rock mass parameter of left bank slope of Longtan Hydropower Station,the influential factors were analyzed with orthogonal analysis of toppling rock slope,the range of dominance of toppling slope,was proposed and the topping condition with displacement vector angle in the same nod of different models were also analyzed.Through contrast with the practical engineering case,the obtained results were shown to be applicable as a quick analysis method for failure...

Based on the engineering geologic section model and rock mass parameter of left bank slope of Longtan Hydropower Station,the influential factors were analyzed with orthogonal analysis of toppling rock slope,the range of dominance of toppling slope,was proposed and the topping condition with displacement vector angle in the same nod of different models were also analyzed.Through contrast with the practical engineering case,the obtained results were shown to be applicable as a quick analysis method for failure mode of toppling rock slope.

借助广西龙滩水电站左岸边坡工程地质剖面模型以及岩体参数对反倾岩质边坡的影响因素进行了多工况分析,提出了边坡反倾优势角的范围。并通过不同模型对应同一点的位移矢量角对层状反倾岩质边坡的反倾条件进行了界定分析。通过与工程实例总结资料对比,其可用性强,并为反倾岩质边坡的快速破坏模式分析提供了科学依据。

 
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