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重组cho细胞
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  recombinant cho cell
     Effects of Glucose on Growth, Metabolism and EPO Expression in Recombinant CHO Cell Cultures
     葡萄糖对重组CHO细胞生长代谢及EPO表达的影响
短句来源
  recombinant cho cells
     Efficient Expression of IL-2Rα in Recombinant CHO Cells
     重组CHO细胞高效表达人IL-2Rα链的研究
短句来源
     Inhibition of HBsAg Expression in Recombinant CHO Cells by Vector-mediated RNAi
     应用载体介导的RNAi技术抑制HBsAg在重组CHO细胞中的表达
短句来源
     Effect of Metabolite on HBsAg Secretion of Recombinant CHO Cells
     重组CHO细胞培养过程中代谢产物对细胞分泌HBsAg的影响
短句来源
     Semi continuous culture of recombinant CHO cells which can secret prourokinase(Pro UK) was carried out in a 100mL and a 1000mL spinner flasks using this kind of microcarriers. During the 25 days cultivating process in a 100mL spinner flasks,the cell density reached 6.3×10 6/mL,the maximal activity of Pro UK was 2325 IU/mL and the Pro UK production was 28.7mg.
     利用该微载体培养能分泌尿激酶原(ProUK)的重组CHO细胞,在100mL搅拌瓶中换液培养25d,细胞最高密度为63×106/mL,尿激酶原最高活性为2325IU/mL,共获287mg产品。
短句来源
     Influence of Glucose, Sodium Butyrate and Lactate on Glycosylation of EPO Expressed in Recombinant CHO Cells
     葡萄糖、丁酸钠和乳酸对重组CHO细胞表达EPO糖基化的影响
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  “重组cho细胞”译为未确定词的双语例句
     Results Decreased expression levels of HBsAg were determined in the culture supernatant of CHO cells 24-120 h after transfection with plasmids pHs-9 and pHs-170 respectively.
     结果pHs-9和pHs-170转染重组CHO细胞后24~120h,在细胞培养上清中检测到HBsAg表达降低,抑制率均达到70%左右。
短句来源
     Study on High-level Expression of Recombinant Hepatitis B Surface Antigen in CHO Cell
     重组CHO细胞高效表达乙肝表面抗原的研究
短句来源
     Optimizing the Recombinant CHO HBsAg Purification
     重组CHO细胞HBsAg纯化工艺的优化
短句来源
     Methods Design and synthesize two pairs of 64 nt oligo nucleotide fragments according to the sequence of HBV gene and insert downstream to H1 promoter to construct plasmids pHs-9 and pHs-170 for expression of short hairpin RNA(shRNA). Transfect CHO cells integrated with HBsAg gene by the two plasmids and determine the expression level of HBsAg by ELISA.
     方法设计两段靶向HBV s基因的特异性siRNA,合成两对64nt寡核苷酸,插入载体H1启动子下游,构建表达干扰性发夹状RNA(Short hairpin RNA,shRNA)的载体pHs-9和pHs-170,转染重组CHO细胞(该CHO细胞整合了HBV s基因,可稳定表达s抗原),用ELISA法检测HBsAg的表达。
短句来源
     Objective:To optimize the method of recombinant CHO HBsAg purification.
     目的 :优化重组CHO细胞HBsAg纯化工艺。
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  recombinant cho cell
A recombinant CHO cell line producing human prorenin was cultivated on microcarriers in serum-free medium.
      
A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin
      
A recombinant CHO cell line, CHO2DS, was immobilized on porous microcarrier Cytopore 1 and cultivated in 1 l modified Super-spinner and 2 l stirred tank bioreactor with the perfusion of a low-cost chemically defined protein-free medium DF6S.
      
The transiently expressed protein had the same extent of glycosylation as the same antibody produced from a stably transfected recombinant CHO cell line.
      
A recombinant CHO cell line (GT19) secreting a high level of human growth hormone (hGH) was constructed with amplification of the introduced hGH gene.
      
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  recombinant cho cells
However, when treated recombinant CHO cells were passaged for 10 d and then subcloned, no conversion could be detected when >amp;gt;90 clones were analyzed by locus-specific PCR-RFLP.
      
Examples are presented for the use of this protocol for recombinant CHO cells.
      
Continuous production of tissue plasminogen activator from recombinant CHO cells in a depth filter perfusion system
      
The growth rate of recombinant CHO cells at 10-7 M MTX was found to be 20% lower than that of recombinant CHO cells in MTX-free medium, and the cell growth rate at 10-6 M MTX was 20% lower than that of recombinant CHO cells at 10-7 M MTX.
      
The reduction of growth rate in recombinant CHO cells is therefore thought to be mainly due to the effect ofdhfr and foreign gene amplification and increased β-galactosidase expression.
      
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Human interleukin-2 recptor a chain (IL-2Rα) cDNA was transfected into CHOdhfr~- cells in which it had been expressed stably. 13 CHOdhfr~+ transformants with efficient expression (OD 490>0. 80) were screened fron 180 CHOdhfr~+ clones by ELISA. For gene amplification, cultures were exposed to methotrexate (MTX) at different concentration and 3 clones of more efficient expression (R30-6,R30-27 and R30-159) were obtained with OD490 value of 1. 95,1. 86 and 1. 66,respectively. The cell culture test indicated that...

Human interleukin-2 recptor a chain (IL-2Rα) cDNA was transfected into CHOdhfr~- cells in which it had been expressed stably. 13 CHOdhfr~+ transformants with efficient expression (OD 490>0. 80) were screened fron 180 CHOdhfr~+ clones by ELISA. For gene amplification, cultures were exposed to methotrexate (MTX) at different concentration and 3 clones of more efficient expression (R30-6,R30-27 and R30-159) were obtained with OD490 value of 1. 95,1. 86 and 1. 66,respectively. The cell culture test indicated that no changes of primary efficient expression were assayed in 30 passages and the effect of gene amplification with MTX could exist within 15 - 20 passages. The molecular weight of rIL-2Rα is about 50 - 55000 dal-ton and it is able to bind IL-2 and anti-Tac McAb. Southern blotting demonstrated that the raising of recombi-nant production by MTX resulted from increses of foreign gene copy rather than appearence of reintegration and rearrangment. Therefore, the results could be as the strong basis of rIL-2Rα production by fermenting CHO cells.

将人IL-2Ra cDNA导入CHOdhfr~-细胞,经ELISA对180个重组克隆的鉴定,筛选出13株高产克隆,其OD值均大于0.80。再经MTX扩增基因试验,获得了3株更高表达水平的克隆,其OD值分别为1.95、1.86及1.66。稳定性试验表明:MTX诱导前的高效表达水平在30代次时未见改变,MTX的扩增基因效果可维持15—20代次,rIL-2Ra的分子量为50—55kd,具有Tac抗原性和结合IL-2的能力。Southern分析证实:MTX是通过扩增外源基因的份额来提高重组产物的表达水平。本试验为发酵培养重组CHO细胞、大量制备IL-2Ra蛋白奠定了坚实基础。

Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-derivedHBsAg...

Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of "a" determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-derivedHBsAg (CD HBsAg ) and vaccinia-derived HBsAg (VD HBsAg). There was alsosimilar density of epitopes on two preparations of yeast-derived HBsAg (YDHBsAg) produced by Amgen Co. and Phillipe Co. respectively. However epi-topes against three McAbs (B_6, B_9 And A_1) on YD HBsAg were quantitativelydifferent from those on PD HBsAg, CD HBsAg and VD HBsAg. This analysisof HBsAg epitopes may be important for the evaluation of their immunogen i-sity.

通过Western-Blot发现,多克隆抗-HBs抗体及单克隆抗体B_(13),A_1均识别24kd及27kd两条多肽带,而单克隆抗体B_6,B_9及B_(12)只识别24kd,不识别27kd的多肽带。用这几株单克隆抗体对不同来源HBsAg a决定簇的表位密度进行了研究,结果表明血源、重组CHO细胞表达及重组痘苗病毒表达的HBsAg与重组酵母表达的HBsAg在结合单克隆抗体A_1、B_6及B_9的密度上有数量的差别,并且有统计学意义。

With ELISA it was identified that IL-2R?produced in recombinant CHOdhfr" cells are mainly existed on the surface of membinant and the others are secreted into medium as soluble IL-2R(sIL-2R). The time courses of sIL-2R expressed in CHOdhfr" cells is similar to a longer constant stage with high level. The results show that the metabolism of mIL-2R is more active than that of sIL-2.and no obvious change of sIL-2R was investigated when the level of mIL-2Roc was increasing. This typical metabolism rule in sIL-2R...

With ELISA it was identified that IL-2R?produced in recombinant CHOdhfr" cells are mainly existed on the surface of membinant and the others are secreted into medium as soluble IL-2R(sIL-2R). The time courses of sIL-2R expressed in CHOdhfr" cells is similar to a longer constant stage with high level. The results show that the metabolism of mIL-2R is more active than that of sIL-2.and no obvious change of sIL-2R was investigated when the level of mIL-2Roc was increasing. This typical metabolism rule in sIL-2R may appear a specific magnificience of immuno-biology. In addition,the analysis of RIA shows the Bmax of rIL-2Rα is 3 300/cell,KD is 1. 5× 10-11M. And it is needed much more IL-2 to block Wu69 McAb binding to IL-2Ra玾hich indicates that the binding sites of IL-2 and Wu69 McAb to IL-2Ra are difference.but near to each other.

ELISA检测表明:重组CHO细胞产生的IL-2Rα分子主要分布在细胞膜上,少量分泌到膜外成为可溶性IL-2R(sIL-2R)。sIL-2R产生的时相与膜IL-2Rα相似,但后者有一个较长的高浓度稳定期。此结果提示:膜IL-2Ra代谢比培养液中sIL-2R明显活跃,但sIL-2R的水平并不随膜IL-2Rα含量增加而改变,sIL-2R这种恒定的代谢方式可能包含着独特的免疫生物学意义。放免受体分析表明:重组IL-2Rα的最大结合容量(Bmax)为33000/细胞,KD为1.5×10~(-11)M。大剂量IL-2才能阻断Wu69McAb与IL-2Rα的结合。提示:IL-2与Wu69 McAb结合IL-2Rα的位点虽然不同,但可能十分靠近。

 
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