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改良sds法
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  “改良sds法”译为未确定词的双语例句
     The improved CTAB extraction method which was designed by our research group is suitable to extract the high quality DNA from wheat. Facile operation can save time and be economical.
     改良CTAB法提取DNA质量好于改良SDS法,改良方法尤其改良CTAB法是一种适合小麦基因组DNA提取的方法。
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     1.Based on the analysis of three isolation methods for genomic DAN of prunus, the results showed that high-salt, low-pH method is optimal for isolation method.
     1.用高盐低pH法、改良SDS法和CTAB法三种方法提取李的幼嫩叶片基因组DNA,结果表明高盐低pH法是较好的提取李基因组DNA的方法。
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     Total RNA was prepared from the leaves with higher chitinase activity by methods of Trizol,guanidine isothiocyanate and SDS,and RT-PCR reaction was carried out.
     分别采用Trizol试剂法、改良SDS法、异硫氰酸胍法等方法对酶活力最高试验组的叶片提取RNA进行RT-PCR反应。 结果表明,0.6mmol.
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     The results showed that both the modified SDS and CTAB methods are better than the original SDS and CTAB methods. Yield of genomic DNA was not only related to extraction methods,but also to cotton tissues.
     本文以多酚类植物棉花为研究材料,对SDS法、CTAB法、改良SDS法和改良CTAB法进行了比较研究,结果表明:改进的SDS法和改进的CTAB法分别优于原SDS法和原CTAB法,两种改进的方法对提取棉花基因组DNA有异曲同工之妙。
     The main results are as follows:1 .Based on the analysis of three pomelo genomic DNA isolated methods. The improving SDS method is best one.
     1.用改良SDS法、高盐低pH法和CTAB法三种方法提取柚的幼嫩叶片基因组DNA,结果表明改良SDS法是较好的提取方法。
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     An Improved Method for Staining-destaining of SDS-PAGE
     SDS-PAGE染色-脱色方改良
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     An improved method for SDS-PAGE and its application
     SDS-聚丙烯酰胺凝胶电泳技术的改良及其应用
短句来源
     The improvement A.
     改良 A .
短句来源
     In breeding of polyploid of Brassica oleracea L.var.
     用改良L.
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     An Improved Method for Extraction and SDS-PAGE of Total Protein in Cotton Leaves
     棉叶总蛋白提取及SDS-PAGE电泳的改良
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The DNA samples of Chinese cabbage male sterile line “CMS3411 7” and maintainer line “3411 7” were prepared by SDS ,Urea,improved SDS,Benzyl chloride and CTAB methods,respectively.The results showed that DNA samples obtained by the improved SDS method possessed the standard ultraviolet absorbance spectrum of pure natural DNA,whose A 260 /A 280 was 1.70-1.90 and A 260 /A 230 was 1.8-2.0.The yield of DNA obtained by the improved SDS method was 400 μg/g young leaves,and the DNA samples prepared...

The DNA samples of Chinese cabbage male sterile line “CMS3411 7” and maintainer line “3411 7” were prepared by SDS ,Urea,improved SDS,Benzyl chloride and CTAB methods,respectively.The results showed that DNA samples obtained by the improved SDS method possessed the standard ultraviolet absorbance spectrum of pure natural DNA,whose A 260 /A 280 was 1.70-1.90 and A 260 /A 230 was 1.8-2.0.The yield of DNA obtained by the improved SDS method was 400 μg/g young leaves,and the DNA samples prepared by this procedure were suitable for RAPD analysis.

对用 SDS法、尿素法、改良 SDS法、氯化苄和 CTAB法提取的大白菜基因组DNA进行了比较。结果表明 ,改良 SDS法提取的 DNA具有典型的天然 DNA分子的标准紫外吸收光谱特点 ,其 A2 6 0 /A2 80 为 1 .7~ 1 .9,A2 6 0 /A2 30 为 1 .8~ 2 .0 ,叶片的 DNA产量为 40 0μg/g。用该法提取的大白菜 DNA适于进行 RAPD分析。

Based on the analysis of several isolation methods for genomic DNA of Chinese chestnut,it was found that the improved SDS method was optimal for RAPD and other molecular biology researches.

通过对板栗基因组 DNA几种提取方法获得的 DNA质量分析 ,表明改良 SDS法是最为理想的提取方法 ,能满足 RAPD及其他分子生物学研究的需要

The effects of the DNAs,extracted from young leaves and buds(or sprouts)of Musa by modified SDS,PVP and CTAB methods,the primers and their concentrations,the concentrations of dNTPs,Taq DNA polymerase,annealing temperature and time,number of amplifying cycles,etc.,on RAPD analysis in Musa were studied. The results showed that the amplified results were the same on the whole,although the quantitative and qualities of the DNAs of young leaves or buds extracted by modified SDS,PVP and CTAB methods were different;and...

The effects of the DNAs,extracted from young leaves and buds(or sprouts)of Musa by modified SDS,PVP and CTAB methods,the primers and their concentrations,the concentrations of dNTPs,Taq DNA polymerase,annealing temperature and time,number of amplifying cycles,etc.,on RAPD analysis in Musa were studied. The results showed that the amplified results were the same on the whole,although the quantitative and qualities of the DNAs of young leaves or buds extracted by modified SDS,PVP and CTAB methods were different;and the amplified results of different plants belong to the same clone were similar;and then the optimal amplification conditions for Musa were the following:20 ng template DNA,0 2 mM dNTPs,0 32 pM primer,1 U Taq DNA polymerase,2 5 μL 10×buffer in 25 μL reaction volume. And 45 cycles of 94 °C for 1 min,37 °C for 1 min and 72 °C for 1 5 min,then 72 °C for 10 min,4 °C storing. In the 249 arbitrary primers used in experiment,only 18 primers were amplified 3~8 clear bands in all the 7 strains of Musa.

比较了不同提取方法对香蕉植株不同部位组织提取 DNA的质量及其 PCR扩增结果 ,对香蕉 RAPD分析中引物种类和浓度 ,复性温度 ,d NTPs,Taq DNA聚合酶浓度 ,热循环数等因素进行了比较影响分析。结果表明 ,虽然改良的 SDS法、CTAB法和 PVP法提取的植株嫩叶和吸芽 DNA提取量和纯度各不相同 ,但其 PCR扩增结果基本相同 ;相同克隆不同植株的 DNA其 PCR扩增结果也基本相同 ;建立了适合香蕉大规模 DNA多态性分析 RAPD反应体系 :2 5 μL反应液中 ,含 1倍缓冲液 ,0 .2 m Md NTPs,0 .3 2 p M随机引物 ,IUTaq酶 ,2 0 ng模板 DNA;反应循环数为 45 ,热循环条件为 94°C,1 min;3 7°C,1 min;72°C,1 .5 min;之前为 94°C,5 min;之后为72°C,1 0 min。在筛选的 2 49个随机引物中 ,有 1 8个在 7个品种上都能扩增出 3~ 1 0条比较清晰条带。

 
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