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  sds method
Optimization of CE-SDS Method for Antibody Separation Based on Multi-Users Experimental Practices
      
Capillary Electrophoresis-Sodium Dodecyl Sulfate (CE-SDS) method with UV detection was developed and satisfactorily used for determination of purity and manufacturing consistency of a monoclonal antibody (MAb) at Amgen Inc.
      
The CE Users Forum first identified the issues which needed resolution, defined group experiments to better understand the problem and to test potential solutions, and together defined a generic (platform) CE-SDS method for MAbs.
      
We present here a platform CE-SDS method for purity determination of MAbs.
      
The optimized CE-SDS method is used during process development and has been transferred to the quality control (QC) lab as a purity assay for lot release testing of therapeutic antibodies.
      
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The method of isolating genomic DNA and RAPD primer screening of were studied. The results are as follows; Qualified DNA could be obtained from Litsea coreana var. lanuginose by means of the improved SDS method. The DNAs are qualified for RAPD analysis. At.the same time, 16 arbitrary 10 - mer primers were selected from 4 groups to show much higher genetic diversity of L. coreana var. lanuginose.

以毛豹皮樟叶片为材料,对其DNA提取方法和RAPD引物的筛选进行了研究。结果表明,采用改良SDS法从毛豹皮樟的老、幼叶片中都能提取到高质量的DNA,可以用于RAPD分析;从4组随机引物中筛选出了16个显示毛豹皮樟遗传差异的多态性引物。

A procedure for isolation of plant total DNA from leaf of Robinia pseudoacacia with a modified SDS method is introduced. Comparing to the traditional methods, it is simple to operate, higher content and good integrity of DNA. It is suitable to prepare plant DNA for the genetic engineering.

以刺槐叶片为材料,摸索出了一套改良的SDS法。与传统方法相比,该方法DNA收取量大,操作简单、纯度高、完整性好,适用于遗传工程的DNA制备。

 
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