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肾脏系膜细胞
相关语句
  glomeruli mesangial cells
     Study on the Preparation of Effective Fraction of SKS and Their Effect on the Proliferation of Glomeruli Mesangial Cells
     肾康舒有效部位的制备方法与对肾脏系膜细胞增殖影响的研究
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     Methods GMC were cultured in vitro and the groups were designed as follows: low glucose group(NG, 5.5 mmol/L d-glucose), high glucose group(HG, 30 mmol/L d-glucose), valsartan group (HG+val, 30 mmol/L d-glucose+valsartan 10μmol/L) and mannitol group (MG, 24.5 mmol/L mannitol +5.5 mmol/L d-glucose).
     方法原代培养大鼠肾脏系膜细胞,随机分为4组:低糖组(NG,d-葡萄糖5.5 mmol/L)、高糖组(HG,d-葡萄糖30 mmol/L)、甘露醇组(MG,d-葡萄糖5.5 mmol/L+甘露醇24.5 mmol/L)和缬沙坦组(HG+Val,d-葡萄糖30 mmol/L+缬沙坦10μmol/L)。
短句来源
     ObjectiveTo compare the effect of glucose in high or low concentrations on the expression of extracellular matrix in mesangial cells in vitro, investigate the effect of complement factor H and adrenomeduUin on rat mesangial cells cultured under high glucose condition, and observe the effect of diovan, atovastatin and pioglitazone on the expression of complement factor H and adrenomeduUin.
     通过体外培养实验观察高浓度与低浓度葡萄糖对肾小球系膜细胞TGF-β_1以及细胞外基质表达的影响,以及肾上腺體质素和补体因子H对高糖条件下培养的大鼠肾脏系膜细胞TGF-β_1以及细胞外基质过度表达的影响,并且观察应用代文、阿托伐他汀和匹格列酮干预后补体因子H和肾上腺髓质素表达的变化。
短句来源
     Objective To study the effect of aldose reductase (AR) on expression of fibronectin and collagen Ⅳ in cultured rat renal mesangial cells (MsC).
     目的探讨醛糖还原酶(AR)对大鼠肾脏系膜细胞(MsC)表达细胞外基质成分纤连蛋白(FN)和Ⅳ型胶原(ColⅣ)蛋白的影响,以及AR在肾小球硬化病变过程中的可能作用及其机制。
短句来源
     TGF-β1 Mediates the Regulation of the Expression of MMP-2 in Cultured Rat Mesangial Cell
     TGF-β1参与全反式维甲酸对大鼠肾脏系膜细胞MMP-2表达的调节
短句来源
     Effects of aldose reductase on the expression of fibronectin and collagen Ⅳ in cultured rat renal mesangial cells
     醛糖还原酶对大鼠肾脏系膜细胞表达纤连蛋白和Ⅳ型胶原蛋白的影响
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     Stem Cells in the Kidney
     肾脏细胞
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     Methods Quiescent rat mesangial cells (MCs) were treated by aldosterone.
     方法用醛固酮刺激大鼠肾脏系膜细胞
短句来源
     Effect of Emedin on the expression of proliferating cell nuclear antigen (PCNA): Cyclin during the mesangial cell cycle
     大黄素对肾脏系膜细胞增殖细胞核抗原的影响
短句来源
     We observed the effect of the inhibition on cell proliferation and apoptosis, then explored the apoptosis pathway.
     观察FN的缺失对大鼠肾脏系膜细胞增殖与凋亡的影响。
短句来源
     ②enlargement of the kidneys with thickening of GBM, increase of mesangial matrix and tubular degeneration;
     肾脏增大,肾小球基底膜增厚,系膜基质及系膜细胞增加,肾小管变性;
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In this paper, the method of cultured rat mesangial ceils (Ms ceils) and morphologic analysis of Ms cells with DMPS membrane were firstly established. The effect of Cyclosporine A (CsA)on the Ms cells contraction and the protection of Ms cells from CsA toxicity using platelet-activating factor (PAF) antagonist-BN52021 were observed. In results, CsA induced Ms cells contraction in time and concentration dependent manner. CsA (10~(-9)M) also had the effect and lasted half an hour. Twenty minutes after removing...

In this paper, the method of cultured rat mesangial ceils (Ms ceils) and morphologic analysis of Ms cells with DMPS membrane were firstly established. The effect of Cyclosporine A (CsA)on the Ms cells contraction and the protection of Ms cells from CsA toxicity using platelet-activating factor (PAF) antagonist-BN52021 were observed. In results, CsA induced Ms cells contraction in time and concentration dependent manner. CsA (10~(-9)M) also had the effect and lasted half an hour. Twenty minutes after removing of CsA from cells, the Ms cells could recover from their contracting states. Ms cells contraction induced by CsA was significantly inhibited by PN52021, at 5×10~(-5)M. The results indicate: 1) The effect of CsA induced Ms cells contraction is reversible and the contraction of Ms cells can reduce renal blood ilow and glomerular capillary kf in rive. 2) PAF may take part in the cell contraction in some way. PAF antagonist BN52021 has the effect on prophylaxis and treatment of CsA nephrotoxicity.

本文采用SD大鼠肾脏系膜细胞培养及硅胶膜形态分析技术。研究环孢素A(CsA)诱导肾系膜细胞收缩作用及血小板活化因子拮抗剂BN52021对CsA诱导作用的抑制效应。结果示CsA引起系膜细胞收缩呈现时间和剂量依赖效应,其作用可逆。BN52021浓度为5×10~(-5)M时,可显著地抑制CsA的诱导作用。结果提示CsA通过可逆性诱导系膜细胞收缩引起肾小球毛细血管血流量和超滤系数(Kf)下降;血小板活化因子可能参与了这个过程,使用血小板活化因子拮抗剂可能有预防及治疗CsA肾毒性的作用。

To study the effect of captopril on renal protection,the intracellular Ca2+ level of rat mesangial cells cultured in vitro was detected with laser scanning confocal microscopy. The results showed thatcaptopril directly decreases the Ca2+concentration of glomerular mesangial cells,which demonstrated a closecorrelation between curves for inhibitive effect of captopril on the fluorescence signals of cytoplasmic Ca2+and concentration of captopril. This finding might be an important contribution to the mechanism...

To study the effect of captopril on renal protection,the intracellular Ca2+ level of rat mesangial cells cultured in vitro was detected with laser scanning confocal microscopy. The results showed thatcaptopril directly decreases the Ca2+concentration of glomerular mesangial cells,which demonstrated a closecorrelation between curves for inhibitive effect of captopril on the fluorescence signals of cytoplasmic Ca2+and concentration of captopril. This finding might be an important contribution to the mechanism of the renal protective effect of ACEI.

通过体外培养肾小球系膜细胞的方法,采用激光共聚焦显微镜技术,测定细胞内游离Ca2+水平。结果发现卡托普利对肾脏系膜细胞钙有直接抑制作用,其引起的细胞内Ca2+荧光强度下降反应曲线与药物浓度有一定关系。血管紧张素转换酶抑制剂对细胞内Ca2+的这种抑制作用可能是这类药物对肾脏保护作用的众多机制之一。

Objective To provide a

目的为特异性地将病毒性白细胞介素10(vIL10)基因转移到肾脏提供一种手段。方法应用逆转录病毒载体介导的基因转移技术将外源性基因vIL10转移到大鼠肾脏系膜细胞,应用RTPCR、ELISA方法检测其表达,并通过对混合淋巴细胞反应(MLR)的影响对其活性进行分析。结果vIL10在转染的系膜细胞表达20000pg·(106细胞)-1/24h,应用3HTdR掺入法检测vIL10对MLR的影响,实验组明显低于对照组(P<005)。结论外源性基因在系膜细胞有效地转录和表达,并对MLR有抑制作用。

 
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