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   间接碘标记法 在 特种医学 分类中 的翻译结果: 查询用时:2.013秒
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间接碘标记法    
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  indirect labelling
The directly 15N-labelled casuarina residue and indirect labelling (unlabelled casuarina + 15N soil) were compared to estimate the proportion and amount of N derived from the residue and fertilizer.
      
Using the indirect labelling PAP-technique, specific immunostaining was found in the endothelial layer of all arteries and arterioles of kidney cortex and in some descending vasa recta.
      
The indirect labelling of antibodies, with the avidin-biotin amplifying system, should make this technique suitable for the quantitation of other plant growth substances against which specific antibodies are available.
      
Direct and indirect labelling procedures were employed using colloidal gold and ferritin-labelled lectins, or biotinylated lectins followed by gold-labelled streptavidin.
      
by means of a MiniMACS separation unit after indirect labelling with the antibody mAb414 (raised against rat liver nucleoporin p62) and a secondary anti-mouse antibody labelled with superparamagnetic MACS Microbeads.
      
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  indirect labelling
The directly 15N-labelled casuarina residue and indirect labelling (unlabelled casuarina + 15N soil) were compared to estimate the proportion and amount of N derived from the residue and fertilizer.
      
Using the indirect labelling PAP-technique, specific immunostaining was found in the endothelial layer of all arteries and arterioles of kidney cortex and in some descending vasa recta.
      
The indirect labelling of antibodies, with the avidin-biotin amplifying system, should make this technique suitable for the quantitation of other plant growth substances against which specific antibodies are available.
      
Direct and indirect labelling procedures were employed using colloidal gold and ferritin-labelled lectins, or biotinylated lectins followed by gold-labelled streptavidin.
      
by means of a MiniMACS separation unit after indirect labelling with the antibody mAb414 (raised against rat liver nucleoporin p62) and a secondary anti-mouse antibody labelled with superparamagnetic MACS Microbeads.
      
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~(125)I-LEP was prepareded by ~(125)I with indirect iodination and direct iodination respectively,to develop RIA for method comparison.For indirect iodination,BH agent was iodinated by ch-T,then conjugated with leptin.Specific radioactivity of ~(125)I-LEP was 1.43 MBq/μg,total labelling rate of ~(125)I-37.8%.The B_(max) of ~(125)I-LEP-97.5%,NSB-6.7 % and B_0-43.7%.The sensitivity was 0.25μg/L,CV of within and between assay-7.8% and 12.9%,respectively.The recovery rate was 96.4%~103.2%,there was no cross-reaction...

~(125)I-LEP was prepareded by ~(125)I with indirect iodination and direct iodination respectively,to develop RIA for method comparison.For indirect iodination,BH agent was iodinated by ch-T,then conjugated with leptin.Specific radioactivity of ~(125)I-LEP was 1.43 MBq/μg,total labelling rate of ~(125)I-37.8%.The B_(max) of ~(125)I-LEP-97.5%,NSB-6.7 % and B_0-43.7%.The sensitivity was 0.25μg/L,CV of within and between assay-7.8% and 12.9%,respectively.The recovery rate was 96.4%~103.2%,there was no cross-reaction with TSH,LH,FSH,PRL,GH and Ins,the correlation coefficient between results of indirect iodination method and that of Linco Kit was 0.951.Standard curve stability of ~(125)I-LEP prepared by indirect iodination was much better than that by the direct iodination in 45 days,which can satisfy the needs in clinic use.

间接碘标记法与直接碘标记法标记消脂素(leptin,LEP),并分别建立LEP的RIA进行方法学的比较。间接碘标记法先以氯胺T法标记对羟基苯丙酸琥珀酰亚胺脂(BH试剂)制备125I-BH,并与LEP进行联结制备125I-LEP。125I-LEP放射性比活度为1.43 MBq/μg,125I的总标记率为37.8%,125I-LEP的最大结合率(Bmax)为97.5%。所建立的RIA的NSB为6.7%,零计量管(B0)的结合率(B/T)为43.7%,灵敏度为0.25μg/L,高、中、低三种浓度的样品批内CV为7.8%,批间CV为12.9%,回收率为96.4%~103.2%,与TSH、LH、FSH、PRL、GH及Ins等均无交叉反应,与Linco的药盒进行临床测试对比亦显示良好的线性关系,r为0.951,以间接碘标记法标记的125I-LEP在45天内其标准曲线的稳定性较直接法标记的125I-LEP有较大的提高,能满足临床工作的需求。

 
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