Four short random repeat loci (BM1862?BM2113?BM720 and TGLA122) were used for individual identification of two pieces of frozen semen lack of marks and four bulls.
Comparing with traditional methods of pellets,the quality of the freezing semen remarkably improved,the motility of the thawed semen increased from 35.45% to 57.88%(P<0.01),the intact acrosomes rate elevated from 42.25% to 78.24%(P<0.01).
Oestrum pregnancy rate was 76.42%, compared with Australian Dr Salaman result (74.04%), increased 2.38%(p>0.05), compared with ordinarily freezing semen inseminated result (the highest level 55.45%), increased 20.97%(p<0.01). The experiment result showed that inseminated to uterine cornu with laparoscope is very significant for eve's pregnancy.
Two diluents that can be used in fox semen freezing were selected in anexperi-ment,the semen dosage for frozen pellet semen and the defrosting temperature were 0.1mland 42~45℃ resp,the semen may be frozen on fluorine board or copper net.
In this paper it is pointed that the technological key in the processing proce-dures of hull semen freezing is the establishment of temperature curve consisted of thefreezing temperature and the coolingrate.
the average number of microbe in pellet freezing sperm (0.1ml、0.5ml) were 920 and3750 , in tubule freezing sperm the number (0.25ml、0.5ml) were 1060 and 1520.The sorts ofmicrobe had Grape、Intestines bacillus、Streptococcus and so on.
The methods occur many problem that traditionally Tibet cattle×yak, natural mating between imported breed and local yak, freezing sperm and artificial fertilization.
The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied.
Furthermore, we shall show how the evaluation of the results of a frozen semen specimen, on the basis of analysing a single straw after such freezing, does not guarantee the homogeneity of all the straws.