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酶活
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  enzyme activity
    Effect of Modification of L-asparaginase by Chitosan on Enzyme Activity and Antigenity
    壳聚糖修饰L-天门冬酰胺酶对酶活及抗原的影响
短句来源
    The purified hPK enzyme activity was mensurated after prohPK was activated by trypsin. The hPK specific activity reached 5.6U/mg.
    纯化得重组hPK经Trypsin进行酶原活化后测定酶活,比活达5.6U/mg。
短句来源
    And the enzyme activity in residual liquid is still high, which means the enzyme is still not immobilized uncompletely and this process needs to be optimizated in further.
    同时发现经过固定化载体处理后的残酶液还表现出较高的酶活,说明固定化尚不完全,需要进一步优化固定化条件或者进行二次固定化。
短句来源
    When the concentration of Tetrahydrofuran is 60% (V/V) , the enzyme activity can reach its highest value 12.84 X 105U/mg;
    当四氢呋喃浓度为60%(V/V)时,酶活达到最大为12. 84×10~U/mg;
短句来源
    when the concentration of DMC is 40% (V/V) , the enzyme activity can reach its highest value 9.76 X 105U/mg.
    当DMC浓度为40%(V/V)时,酶活达到最大为9. 76×10~5U/mg。
短句来源
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  enzyme activities
    After used 10 times, the enzyme activities of Novozym435 only reduce 19%.
    固定化脂肪酶重复使用10次时,酶活仅下降19%。
短句来源
    Mn 2+ ions enhanced xyl II enzyme activity by 2.7-fold whereas Fe 3+ completely inhibited enzyme activities.
    Mn2 + 对xylⅡ酶反应具有促进作用 ,将酶活提高了 2 7倍 ,而Fe3+ 对该酶反应起完全抑制作用。
短句来源
    Cloning and expression of DAAO in Pichia pastoris were also preliminarily studied. Unfortunately, the enzyme activities of the two recombinant Pichia (intracellular and secreted expression, respectively) were both very low.
    对DAAO在毕赤酵母中的克隆和表达进行了初步研究,但两株毕赤酵母重组菌株(胞内表达型和分泌表达型)均只能表达出很低的酶活
短句来源
    Moreover, the addition of 0.1% etOH in the culture medium influenced the enzyme production,as reflected in the improvement of the oxidation and reduction enzyme activities. Consequently,the higher redox enzyme activities result in the higher conversion ability of the cell.
    在Candida parapsilosis细胞培养阶段添加0.1%乙醇,可以将细胞生物量从44g/L提高到48g/L,并且通过提高细胞中氧化还原酶酶活提高细胞的转化能力。
短句来源
    These data obtained are essential to purify cellulase by high pressure CO2. The experimental results indicate that CO2 pressure is not the key factor of the loss of enzyme activities, but ethanol concentration and temperature markedly influence recovered activities.
    实验表明,CO2压力不是酶活损失的主要因素,乙醇浓度和温度对酶活损失的影响较大.
短句来源
  activity of enzyme
    coli. DH5α and the activity of enzyme solution precipitated by (NH4)2SO4 and cell extract was compared. After precipitation, the activity, protein content and specific activity were 2.73, 1.72 and 1.61 folds, and the crude enzyme was prepared as immobilization metarial.
    其次将已构建的质粒转入大肠杆菌DH5α中发酵培养,考察了硫酸铵沉淀前后P450 BM-3酶活的变化,结果表明,经硫酸铵沉淀后,粗酶的酶活、蛋白含量和比活分别是破胞上清液的2.73、1.72和1.61倍,硫酸铵沉淀起了初步分离和浓缩的作用,所制粗酶作为后续固定化的原料。
短句来源
    within the scopes of pH5~6, the flocculation was effective, and the specific activity of enzyme was relatively higher; rising the temperature was advantage of flocculation, but would result in enzyme deactivation, so the optimum temperature for operation was 20 ℃.
    在pH5~6范围内,絮凝效果好,胰酶比酶活相对较高:升高温度有利于絮凝但会造成酶活损失,故一般选择在20℃左右操作。
短句来源
  “酶活”译为未确定词的双语例句
    The characteristics of Hafnia alvei AS1.1009 decarboxylase were studied. Under the conditions as follows:pH 8.0,temperature 37℃,cell concentration 10 g/L,tween-80 0.5 g/L,substrate concentration 30 g/L,the specific activity was up to 3 840 U.L-lysine can be completely degraded by the decarboxylase for 12 h under the optimal conditions.
    对生物转化中赖氨酸脱羧酶性质研究结果表明该酶最适pH为8.0,最适温度为37℃,吐温-80质量浓度0.5g/L,菌体质量浓度10g/L,底物质量浓度为30g/L时,最高比酶活为3840U。 在此优化条件下转化时间为12h。
短句来源
    Methods of Xylanase Activity Determination
    木聚糖酶酶活测定方法
短句来源
    METHODS FOR XYLANASE ACTIVITY DETERMINATION
    木聚糖酶酶活的具体测定方法
短句来源
    By the above improvement the activity of NHase reached over 6000 U/ml, which was much higher than that in today’s industry.
    使发酵液腈水合酶的活力提高到6000U/mL以上,大大高于现有工业生产中的酶活
短句来源
    3. To enhance the expression level of the recombinant GL-7-ACA acylase, a fragment of the gene, in which the sequence encoding the signal peptide was deleted by PCR method, was used to construct a recombinant plasmid, pET-ACY, and then the recombinant E. coli BL21(DE3)/ pET-ACY.
    为获得高酶活表达,将GL-7-ACA酰化酶基因通过PCR突变的方法去除其信号肽序列,并将其连接到质粒pET-28a上,构建的重组大肠杆菌BL21(DE3)/ pET-ACY能够表达出GL-7-ACA酰化酶活性。
短句来源
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  enzyme activity
The effect of the most promising ones have been looked on the counterparts from mammalian sources and difference in the susceptibility towards enzyme activity inhibition were noted.
      
The results indicated that cancer stem cells show a higher level of enzyme activity than non-stem cells.
      
The Cu2+ had strong restrictive effect on enzyme activity.
      
A rapid but transient decrease in the enzyme activity was observed after 9-12 h after adding glucose to the culture medium.
      
In the first case, the enzyme activity adsorbed on residual solids after extended hydrolysis was used for hydrolysis of the newly added substrate.
      
更多          
  enzyme activities
korshinski plantations to assess the effects of the shrub on the physical and chemical properties of the soil as well as enzyme activities.
      
Inhibitor analysis and studies of enzyme activities with synthetic substrates demonstrated that the culture liquid of Fusarium culmorum contained serine proteinases of various classes.
      
Effects of temperature and pH on the enzyme activities, their stabilities under various conditions, and the kinetics of exhaustive hydrolysis of glucuronoxylan and arabinoxylan were studied.
      
The induction of these enzyme activities depends on the concentration of preparations and plant immune status.
      
The induction of glyoxylate cycle enzyme activities was revealed in the liver and other organs of starving rats.
      
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  activity of enzyme
Studies of hydrolytic activity of enzyme preparations of Penicillium and Trychoderma fungi
      
Microencapsulated PAL has an apparent enzyme activity that is 20% of the activity of enzyme in free solution.
      
Supplementation of fermentation medium with external nitrogen (organic and inorganic) showed a mixed impact on bacterial activity of enzyme synthesis.
      
When the quantity of extract or of cells is increased, the specific activity of enzyme, measured by the Warburg manometric method, decreases.
      
The activity of enzyme recovered by elution after electrophoresis in non-denaturing polyacrylamide gels was wholly dependent on pyridoxamine 5-phosphate.
      
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Instead of l-[(m-nitrobenzyloxy) methyl]pyridinium chloride, β-sulfatoethyl s/&&&lphonyl aniline was used to activate Whatman 3 MM paper and aminobenzene sulphonylethyl paper was obtained. After diazotization poly(I). poly(C) was coupled on diazobenzenesulphonylethyl paper. The poly (I) .poly (C)-paper with capacity of 10-35>μg of poly(I) . poly(C)/cm2 could be used as affinity material to specifically adsorb 2'-5'A synthetase. Under optimal condition (3 mM ATP, 24-40 mM Mg (OAc)2, 30℃ for 18 hours) the bound...

Instead of l-[(m-nitrobenzyloxy) methyl]pyridinium chloride, β-sulfatoethyl s/&&&lphonyl aniline was used to activate Whatman 3 MM paper and aminobenzene sulphonylethyl paper was obtained. After diazotization poly(I). poly(C) was coupled on diazobenzenesulphonylethyl paper. The poly (I) .poly (C)-paper with capacity of 10-35>μg of poly(I) . poly(C)/cm2 could be used as affinity material to specifically adsorb 2'-5'A synthetase. Under optimal condition (3 mM ATP, 24-40 mM Mg (OAc)2, 30℃ for 18 hours) the bound enzyme catalyzed the conversion of more than 30% of ATP into 2'-5'A. The synthesis of 2'-5'A was linearly proportional to the amounts of rabbit reticulocyte lysate used in the assay ranging from 10ul up to 300ul. Compared with the method reported by G.R.Stark et al. this method offered some advantages, simple to prepare β-sulfatoethylsulphonyl aniline readily available and low cost.

poly(1)·poly(C)-滤纸是一种亲和材料,可以用来吸附与双链核酸有亲和力的酶或蛋白。本文介绍用对-β硫酸酯乙砜基苯胺为活化剂制备poly(I)·poly(C)-滤纸的方法。poly(I)·poly(C)的结合容量为10—35μg/cm~2,用来吸附兔网织红细胞裂解液中2’-5’A合成酶效果良好。在一定范围内,酶活与被吸附裂解液量呈线性关系,说明可以用来定量检测未知样品中与poly(I)·poly(C)有亲和力的酶。poly(I)·poly(C)-滤纸在-20℃保存四个月亲和能力不变。本方法与文献报道的方法相比,操作简便试剂易得。

The condition for producing nitrile hydratase by strain JP-1 was investegated. The results suggests that nitrile hydratase is an induced enzyme. The strain JP-1 could utilize propionitrile as sole source of carbon and nitrogen. Enzyme formation was not enhanced by additional carbon sources. Added corn steep liquor could remarkably promote enzyme formation. Propionitrile and propionamide were found to be effective inducers for enzyme formation. Enzyme formatoin was considerably inhibited by Co~(++) ion. when...

The condition for producing nitrile hydratase by strain JP-1 was investegated. The results suggests that nitrile hydratase is an induced enzyme. The strain JP-1 could utilize propionitrile as sole source of carbon and nitrogen. Enzyme formation was not enhanced by additional carbon sources. Added corn steep liquor could remarkably promote enzyme formation. Propionitrile and propionamide were found to be effective inducers for enzyme formation. Enzyme formatoin was considerably inhibited by Co~(++) ion. when the organism grew on a suitable medium consisting of 0.15% corn steep liquor, 0.2% K_2HPO_4·3H_2O, 0.1% NaCl, 0.02% MgSO_4·7H_20, 0.25%(V/V)propionitrile and 0.1%(V/V) trace elements, at pH 7.0 and 28℃ for 48 h, about 3.76 units/ml (or 16035 μg acrylamide/h) of acrylamide was obtained.

对恶臭假单胞杆菌(Pseudomonas putida)JP-1产酶条件进行了研究。结果表明,腈水合酶为诱导酶.该菌株能利用丙腈作唯一的碳、氮源,附加碳源对产酶没有促进作用,但附加玉米浆对产酶则有明显的促进作用.丙腈和丙酰胺是腈水合酶的有效诱导物.Co~(+2)对产酶有明显抑制作用,其它金属离子对产酶无促进作用,JP-1菌产酶最适培养基组成为(%);玉米浆0,15,丙腈0,25(V/V),K_2HPO_4·3H_2O 0.2,NaCl 0.1,MgSO_4·7H_2O 0.02,微量元素0.1(V/V).在pH7.0和28℃条件下培养48h后测得每毫升发酵液酶活为为3.76单位(即16035μg丙烯酰胺/h).

The conditions for expression of penicillin-G-acylase gene using hybrid strain E. coli 108(pPAHD1)were studied in 2-liter automatic fermenter. The growth of the strain and the expression of the penicillin-G-acylase gene were influenced obviously by the dissolved oxygen level in the medium. Experiments revealed that a high dissolved oxygen level no less than 80% saturation was demanded for accumulation of biomass in early log growth phase and it shifted down to 5 % saturation to be compulsory for superproduction...

The conditions for expression of penicillin-G-acylase gene using hybrid strain E. coli 108(pPAHD1)were studied in 2-liter automatic fermenter. The growth of the strain and the expression of the penicillin-G-acylase gene were influenced obviously by the dissolved oxygen level in the medium. Experiments revealed that a high dissolved oxygen level no less than 80% saturation was demanded for accumulation of biomass in early log growth phase and it shifted down to 5 % saturation to be compulsory for superproduction of the enzyme. Signals of variable dissolved oxygen peaks were found of great significance for phenylacetic acid addition. The quantities of phenylacetic acid added were correlated with the peak intervals and the proper dosage used in each addition would bring the concentration to 0.03-0.05%. Under the circumstances of 0.3 kg/cm pressure, 1:0.5v/v/m air flow, defined temperature and other controlled conditions, the level of penicillin-G-acylase activity has been reached 342U/100ml(NIPAB method), which is 1.8 fold higher in comparison with the shaking flask fermentation.

用2升全自动发酵罐观察大肠杆菌工程菌Ec108(pPAHD1)青霉素酰化酶(Penicillin-G-acylase)基因的表达,发现溶解氧浓度对生物量的扩增和基因产物的积累有显著的影响。对数前期扩增生物量时需要相对高的溶解氧,以不低于相对饱和度80%为宜;而产酶阶段则要求相对低的溶解氧环境,可保持相对饱和度5%。按溶解氧峰形变化特征,指导补加苯乙酸的时机;以尖峰出现的间距,决定补加剂量,采用每次补加0.03~0.05%为佳。在罐压0.3kg/cm2,通气比1:0.5及恒温条件下,借助上述手段,最高酶活水平已达342u/100ml(NIPAB法),比摇瓶发酵酶活提高1.8倍。

 
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