The strain DG-4-4 was obtained through UV mutation of a thermostable α-amylase-producing strain DG-4 which was used as the starting strain. The enzyme activity of Dg-4-4 increased by 20.4 %.
Then the strain DG-4-46 was obtained through DES mutation of DG-4-4, the enzyme activity of which increased by 61.9 % than DG-4-4 and by 95 % than DG-4.
The recombined yeast(Saccharomyces cerevisiae BGL-19)could grow on the medium with cellobiose as sole carbon source and the highest enzyme activity(0.967μ/ml)was achieved at 36h.
DETERMINATION OF FILTER PAPER ENZYME ACTIVITY ANDCMC ENZYME ACTIVITY OF CELLULASE
纤维素酶的滤纸 酶活和CMC 酶活的测定
After the three-step treatment, purification fold is 6.7 with 67.14% enzyme activity recovery and single band shows by PAGE determination.
Results showed that the fermentation effect in rotate-drum bioreactor excelled the revolve-shaft one with 0.312g dry biomass, 170 u FPA enzyme activities and 0.074g ethanol production per g dry substrate, which showed a successful scale-up from flasks to bioreactor.
结果显示转鼓式反应器内的发酵效果优于转轴搅拌式，达到0.312g干菌体和170u FPA 酶活／g培养基干重，酒精产率达到0.074g／g培养基干重，各参数均优于摇瓶发酵，说明固态生物反应器放大取得良好效果。
The cellulase producing was used with the method of solid-state culture. The optimum culture conditions of it were as follows:pH4.4～4.6,temperature 28～30℃,moisture content of culture medium 68～70%,and cultivation time4～5day. With such conditions,its enzyme activities of CMCNa reached 9000～12000mgG/g·h.
The optimum reaction condition for the enzyme was at 50℃ and pH 5.5.The relative enzyme activities were above 83% at 50℃ for 8 h and 60% at 60℃ for 2 h.
所产酸性蛋白酶的最佳反应pH5.5,温度50℃,50℃保温8h后其相对 酶活在83%以上,pH5.5、60℃水浴2h后其相对 酶活达60%。
The enzyme’s optimum temperature was about 50℃,and its optimum pH value was about 5.5.Its stabilities of pH and temperature resistance were good ,and its relative enzyme activities were above 83% and 60% in the condition of pH5.5 and 50℃ for 8 hours and pH5.5、60℃ for 2 hours.
该酸性蛋白酶的最佳反应pH为5.5,最佳反应温度为50℃。 50℃条件下保温8h后其相对 酶活在83%以上,pH值为5.5条件下60℃水浴2h后,测定其相对 酶活可达60%。
When incubated with optimum enzyme producing conditions,the β-galactosidase activities of 1.1480 was 0.321 NLU/ml or 2.469 NLU/mg,and the enzyme activities of wch9901 was 0.401 NLU/ml or 6.169 NLU/mg.
在最适产酶条件下,1.1 4 8 0的 酶活力为0.3 2 1NLU/m l粗酶液,比 酶活为2.469 NLU/mg蛋白;
Mg 2+ and Co 2+ could activate enzymatic activity, Al 3+ and Cu 2+ could inhibate the activity, Hg 2+ could inactivate nattokinase quickly.
Mg2 +和Co2 +离子对 酶活有激活作用 ,而Cu2 +、Zn2 +和Al3+等离子对该酶有明显的抑制作用 ;
The optimal technical parameters are as follows: the weight ratio of fresh meat to water is 1 to 2, the added enzymatic activity unit is 3600 units per gram meat, the used enzyme preparation is the mixture of papain 60 and AS1398 with the same activities, the pH value is 6.5-6.8, the reaction temperature is 55 degree centigrade and the reaction time is 6 hours.
Two bacterial strains are obtained,which have enzymatic activity of 5 446 and 12 298 U/L,respectively.
酶活测定表明2株 酶活较高,分别为5 446和12 298 U/L. 经分类鉴定表明一株属于假单胞菌属(Pseudomonas sp.)
A Aspergillus oryzae strain,SA-6,was isolated from different brewing koji samples using the clarity circle method on plate. The fibrinolytic enzymatic activity of SA-6 was 25 U/ml.
利用平板透明圈法作为初筛方法,从不同的酿造曲样中筛选出1株产纤溶酶 酶活较高的米曲霉菌株SA-6,其产纤溶酶 酶活为25U/mL。
The results of experiment showed that to hemicellulose A,the enzymolysis with hemicellulase( enzymatic activity 400IU/g) got a higher productivity than alkali hydrolysis,the yield of xylose was 34.87%,but to hemicellulose B,the productivity of enzymolysis was lower than that of acid hydrolysis,the yield of xylose after hemicellulase-acid hydrolysis was 23.60%.
实验结果显示对半纤维素A用半纤维素酶( 酶活400 IU/g)法水解比用20%NaOH水解效果好,木糖得率达34.87%; 而对于半纤维素B,采用酶法水解比7%H2SO4水解得率相差较大,采用酶解后7%H2SO4水解可得得率为23.60%。
When the concentration in the crude enzyme was 6mg/ml,the activity of enzyme was kept intact and active after 10days storage at 30℃.
Polyphenols can not be combined sufficiently the activity of enzyme decreased slowly because of insufficient cellular fragmentation in the processing of blacke tea congou.
工夫红茶加工过程中β-葡萄糖苷 酶活性在萎凋与揉捻阶段持续上升，揉捻结束达最大值，发酵阶段 酶活持续下降。
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The effect of the most promising ones have been looked on the counterparts from mammalian sources and difference in the susceptibility towards enzyme activity inhibition were noted.
The results indicated that cancer stem cells show a higher level of enzyme activity than non-stem cells.
The Cu2+ had strong restrictive effect on enzyme activity.
A rapid but transient decrease in the enzyme activity was observed after 9-12 h after adding glucose to the culture medium.
In the first case, the enzyme activity adsorbed on residual solids after extended hydrolysis was used for hydrolysis of the newly added substrate.
korshinski plantations to assess the effects of the shrub on the physical and chemical properties of the soil as well as enzyme activities.
Inhibitor analysis and studies of enzyme activities with synthetic substrates demonstrated that the culture liquid of Fusarium culmorum contained serine proteinases of various classes.
Effects of temperature and pH on the enzyme activities, their stabilities under various conditions, and the kinetics of exhaustive hydrolysis of glucuronoxylan and arabinoxylan were studied.
The induction of these enzyme activities depends on the concentration of preparations and plant immune status.
The induction of glyoxylate cycle enzyme activities was revealed in the liver and other organs of starving rats.
The enzymatic activity of the mutant was 188 U/ml at 37°C, which was 80% that of the wild type in the same conditions.
It is possible that this mutation severely impairs enzymatic activity and is the underlying basis for the pathology seen in this patient with Hunter syndrome.
After immobilization, microbial cells retained 79-91% of their initial enzymatic activity.
Pretreatment of plant seeds with kartolin-4 (o-isopropyl-N-2-hydroxyethyl carbamate), a preparation with cytokinin activity, reduced the dehydration-induced inhibition of enzymatic activity.
A New Method of Visualization of the Enzymatic Activity of Flavocytochrome b2 in Electrophoretograms
Studies of hydrolytic activity of enzyme preparations of Penicillium and Trychoderma fungi
Microencapsulated PAL has an apparent enzyme activity that is 20% of the activity of enzyme in free solution.
Supplementation of fermentation medium with external nitrogen (organic and inorganic) showed a mixed impact on bacterial activity of enzyme synthesis.
When the quantity of extract or of cells is increased, the specific activity of enzyme, measured by the Warburg manometric method, decreases.
The activity of enzyme recovered by elution after electrophoresis in non-denaturing polyacrylamide gels was wholly dependent on pyridoxamine 5-phosphate.