In conclusion, reduction of mitotic and MIB-1 indices indicates that suppression of cell proliferation contributes to tumor shrinkage, whereas p27 protein expression and apoptosis play no major role in the adenoma involution.
In addition, we found a weak association of CEACAM1 expression with p27 protein levels (P=0.087 and 0.039), but with none of the other analyzed parameters.
Skp2 expression is associated with down-regulation of p27 protein and cell proliferation in salivary adenoid cystic carcinoma
Moreover, Skp2 small interfering ribonucleic acid (siRNA) transfection decreased Skp2 protein and accumulation of p27 protein and inhibited the cell growth of ACC cells in vitro.
Results: When transferred from monolayer to three-dimensional culture, a consistent upregulation of p27 protein and P-gp protein was observed in ovarian cancer cell lines.
The obtained values are within the limits p = 27-50%, Sv = 2800-6000 cm2/cm3, d = 1.9-6.5 μm, and N = 1.4-10 × 106 cm-2, in agreement with the data fumished by microscopy.
Expression and significance of cyclin D1, p27kip1 protein in bronchioloalveolar carcinoma
Purpose: To investigate the relationship between expression of cell cycle-related protein cyclin D1, p27kip1 and the pathogenesis of bronchioloalveolar carcinoma (BAC) and the value of prediction of prognosis.
Methods: Cyclin D1 and p27kip1 protein were detected by immunohistochemical En Vision method in 43 BACs.
The positivity of p27kip1 in BACs was 51.2% (22/43), significantly lower than that in normal pulmonary tissue (12/13),P>amp;lt;0.01.
An inverse correlation of Skp2 was observed with both its biochemical target p27 expression in gastric carcinoma (rho=-0.451, P=0.000) and with its putative negative regulator, the PTEN tumor suppressor protein (rho=-0.480, P=0.000).
p27 expression had positive relationship with PTEN expression in gastric carcinoma (rho=0.642, P=0.000).
Conclusion: Skp2 overexpression is correlated with carcinogenesis and progression of gastric carcinoma: elevated Skp2 expression is correlated with decreased p27 and PTEN in gastric carcinoma, and p27 expression is parallel with PTEN expression.
Pituitary tumorigenesis is accelerated in mice with severe TGF-β resistance, and greatly accelerated in mice with TGF-β resistance combined with decreased p27 expression compared with wild-type mice.
To better understand the regulatory role of growth factors and hormones in the cell cycle we analyzed cyclin D1, cyclin E, and p27 expression in normal and neoplastic rat pituitary cells.