Isolation of chemical constituents were carried out in step with for effective part of H.petiolulatum by column chromatography. The chemical structures were identified by physical and spectral data analysis. Six constituents have been isolated and established as 3α-epi-betulinic acid (1),Patuloside A (2),dimethylpaxanthonin(3),Morusignin D (4),quercetin (5)and quercitrin (6)respectively for the first time.
Two methods of obtaining 3α-betulinic acid and related compounds from their 3β-epimers were studied: the reaction of bimolecular substitution and the stereoselective reduction of 3-ketoderivatives.
Four compounds were isolated from Coleus carnosifolius. They were identified as betulic acid,betulin,oleanic acid and β sitosterol by spectral analysis and comparison with authentic samples.