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   球蛋白基因启动子 的翻译结果: 查询用时:0.279秒
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球蛋白基因启动子
相关语句
  globulin gene promoter
     Cloning of the Seed Globulin Gene Promoter from Citrus Sinensis Osbeck cv. Jincheng and Analysis in Transgenic Tobacco
     锦橙(Citrus sinensis Osbeck cv.Jincheng)种子球蛋白基因启动子的克隆及在转基因烟草中的功能分析
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  “球蛋白基因启动子”译为未确定词的双语例句
     Cloning and Nucleotide Sequence Analysis of 26kDa Aalpha-Globulin Promoter in Rice (Oryza sativa L.)
     水稻26kDa球蛋白基因启动子克隆及序列分析
短句来源
     Association of (TAAAA)_n repeat polymorphism in promotor of sex hormone-binding globulin gene with glucose metabolic status in patients with polycystic ovary syndrome
     多囊卵巢综合征糖代谢异常与性激素结合球蛋白基因启动子(TAAAA)_n多态性的关系
短句来源
     Analysis of 5'-flanking Deletion and Specific Expression of Citrin Gene Promoter
     柑桔种子球蛋白基因启动子5’端缺失与特异性表达分析
短句来源
     Comparing rice globulin promoter sequence cloned in this experiment with that reported in GenBank AY427575 by using vector NTI software, we found that the DNA fragment composed of 904bp nucleotide with homology 99%.
     测序结果显示,该片段含904个核苷酸对; 采用vectorNTI软件将本试验中克隆的序列与Genbank(AY427575)公布的日本晴球蛋白基因启动子序列比对,有9个核苷酸差异,同源性为99%,证实本试验中克隆的DNA序列为水稻球蛋白基因启动子;
短句来源
     Study on the (TAAAA)n repeat polymorphism in sex hormone-binding globulin gene and the SHBG serum levels in putative association with the glucose metabelie status of Chinese patients suffering from polycystic ovarian syndrome in Shandong province
     性激素结合球蛋白基因启动子(TAAAA)n重复多态及其血清水平与多囊卵巢综合征糖代谢的关联性
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  相似匹配句对
     Cloning and Nucleotide Sequence Analysis of 26kDa Aalpha-Globulin Promoter in Rice (Oryza sativa L.)
     水稻26kDa球蛋白基因启动子克隆及序列分析
短句来源
     4 new genes were obtained.
     U基因
短句来源
     Isolation and characterization of gene promoter from Crambe abyssinica
     海甘蓝基因启动子的分离和鉴定
短句来源
     Cloning of Medicinal Gene and Promoter from Ganoderma
     灵芝药效基因启动子的克隆
短句来源
     Analysis of 5'-flanking Deletion and Specific Expression of Citrin Gene Promoter
     柑桔种子球蛋白基因启动子5’端缺失与特异性表达分析
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  globulin gene promoter
Recombinant REB binds to the region from -754 to -562 in the α-globulin gene promoter, but not to promoters of other major storage genes such as glutelin, prolamin and albumin.
      
A Super-shift assay using anti-REB antibody suggested that REB is a major DNA-binding protein for the α-globulin gene promoter in rice endosperm.
      


About 900bp DNA fragment was amplified by using PCR technique from genomic DNA of rice japonica cultivar"Nipponbare". Recovered and linked with pUCm-T vector, the DNA fragment was transferred to the competence E.coli. After using double enzymes digestion analyses, the recombinant was chosen and the sequence of alpha-globulin promoter was identified. Comparing rice globulin promoter sequence cloned in this experiment with that reported in GenBank AY427575 by using vector NTI software, we found that the DNA fragment...

About 900bp DNA fragment was amplified by using PCR technique from genomic DNA of rice japonica cultivar"Nipponbare". Recovered and linked with pUCm-T vector, the DNA fragment was transferred to the competence E.coli. After using double enzymes digestion analyses, the recombinant was chosen and the sequence of alpha-globulin promoter was identified. Comparing rice globulin promoter sequence cloned in this experiment with that reported in GenBank AY427575 by using vector NTI software, we found that the DNA fragment composed of 904bp nucleotide with homology 99%. There were no differences between the cloned promoter sequence and the sequence published in GenBank in dominant functional regions, based on which we suggested that the nucleotide neutral mutation would result in the main difference between the two sequences of rice globulin promoter of"Nipponbare". The successfully cloning rice globulin promoter sets up bases for the prospective transgenic studies on important crops such as rice.

以水稻品种日本晴基因组总DNA为模板,采用PCR扩增获得约900bp大小的DNA片段,回收该片段并与pUCm-T载体连接,转化感受态大肠杆菌。进行PCR检测和酶切鉴定,选取阳性克隆进行测序分析。测序结果显示,该片段含904个核苷酸对;采用vectorNTI软件将本试验中克隆的序列与Genbank(AY427575)公布的日本晴球蛋白基因启动子序列比对,有9个核苷酸差异,同源性为99%,证实本试验中克隆的DNA序列为水稻球蛋白基因启动子;在重要功能区段上,两者核苷酸序列完全一致。本研究认为造成长期不在一个环境中种植的同一水稻品种球蛋白基因启动子序列差异的原因之一,可能是核苷酸中性突变。水稻球蛋白基因启动子的成功克隆,为今后开展水稻等重要农作物转基因研究奠定基础。

 
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