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凋亡
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  apoptosis
    Proliferation, Differentiation and Apoptosis Molecular Biology Study on the Adjust Mechanism of SD Rat's Marrow Stromal Cell by Sthenia Skeleton Herbs Mixture in Vitro
    补骨合剂对大鼠骨髓基质细胞增殖、分化及凋亡的分子调控机理研究
短句来源
    An Experimental Study of Ginsenoside-Rg3 on Proliferation and Apoptosis of Fibroblast Derived from Pathological Scars
    人参皂甙-Rg3对病理性瘢痕成纤维细胞增殖与凋亡影响的实验研究
短句来源
    Induction of Differentiation and Apoptosis in PC12 and Some Cancer Cells by Toosendanin and the Relative Mechanisms
    川楝素引发PC12等肿瘤细胞分化、凋亡及相关机制的研究
短句来源
    Study of Apoptosis and Autophagy Pathway of Cell Death Induced by Resveratrol
    白黎芦醇诱导细胞死亡的自吞噬和凋亡途径研究
短句来源
    The Impact of Ginkgo Biloba Leaves Extract on the Expression of Apoptosis and the Regulation Gene of Apoptosis during Hepatic Ischemia Reperfusion Injury after Orthotopic Liver Transplantation in Rats
    银杏叶提取物对大鼠肝移植缺血/再灌注损伤凋亡及其调控基因的影响
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  cellar apoptosis
    The changes in myocardial pathology and cellar apoptosis were detected.
    结果 :对照组无细胞变性与坏死 ,仅见极少量凋亡阳性细胞 ;
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  “凋亡”译为未确定词的双语例句
    Studies on the Anti-apoptotic and Antiaging Effects of Ginsenoside Rg1 and Its Mechanism of Action
    人参皂甙Rg1抗凋亡、抗衰老作用及其机制研究
短句来源
    DNA ladder was shown in agarose gel electrophoresis,in additionn to the cells treated by 1.0 μg/mL tanshinone ⅡA .
    琼脂糖凝胶电泳结果显示除1.0μg/mL组外均可见明显的凋亡细胞形成的梯状条带;
短句来源
    The apoptosis-promoting effects of these serum samples(in 2 % concentration) on HSC-T6 after 72 hours in the model were detected by flow cytometry.
    以流式细胞法检测以上采血时间点上2%浓度的血清对乙醛造模后的HSC-T6作用72h后的促凋亡作用。
短句来源
    The apotosis of hippocampi nrurons was detected by Hoechst 33258 kit,the mRNA expression of Bax and Bcl-2 was detected by real time quantitative RT-PCR.
    通过Hoechst试剂盒测定神经元的凋亡,实时定量RT-PCR测定bax和Bcl-2 mRNA的表达。
短句来源
    【Objective】To observe the protective effect of ginsenoside Rg1 on neurons and to explore its mechanism in Alzheimer's disease(AD) cellular model. 【Methods】With NG108-15 cell as the representative of neurons,AD cellular model was induced by β-amyloid protein 25-35(Aβ25-35).
    【目的】探讨在以NG108-15细胞作为神经元代表、β-淀粉样蛋白25-35片段(Aβ25-35)诱导凋亡的阿尔茨海默病体外细胞模型中,人参皂苷Rg1保护神经元的作用及其机制。
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  apoptosis
Prodigiosin also could induce apoptosis of pancreatic cancer cells at low concentration and results in the fragmentation pattern of DNA.
      
All these results demonstrate that prodigiosin can obviously inhibit the proliferation of pancreatic cancer cells H8898 by arresting the cell cycle and inducing apoptosis.
      
Detection of the apoptosis of Jurkat cell using an electrorotation chip
      
The apoptosis of cells is one of the fields that attract increasing attention in biology today.
      
Usually, the cells are treated with chemicals when detecting apoptosis.
      
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Objective. The apoptosisofmurine thymocytes induced by DEX was regulated by LBP and OXY. Methods. PI staining. DNA fragmentation. DNA gel electrophoresis.and so on Results.LBP could inhibit fragmentation of DNA in murine thymocyte induced by DEX.The inhibition is dose-depen-dent and obvious. especially in 1 g/L.Ithas been shown that LBP could greatly reduce the rising of in tracellular Ca2+concentrathn induced by DEX or A23187 respectively.(%of inhibition .54 and 13).Conelusion. LBPcould inhibit the apoptosis...

Objective. The apoptosisofmurine thymocytes induced by DEX was regulated by LBP and OXY. Methods. PI staining. DNA fragmentation. DNA gel electrophoresis.and so on Results.LBP could inhibit fragmentation of DNA in murine thymocyte induced by DEX.The inhibition is dose-depen-dent and obvious. especially in 1 g/L.Ithas been shown that LBP could greatly reduce the rising of in tracellular Ca2+concentrathn induced by DEX or A23187 respectively.(%of inhibition .54 and 13).Conelusion. LBPcould inhibit the apoptosis induced by DEX.which OXY doesn’t have any effect on.

目的:以DEX诱导小鼠胸腺细胞凋亡为模型,研究中药枸杞多糖(LyciumBarbarumPolydsc-charide.LBP)及氧化苦参碱(OXY)对胸腺细胞凋亡的调节作用。方法:应用PI法检测亚2倍体细胞,二苯胺法测定胸腺细胞DNA片断化%及DNA凝胶电泳。结果:LBP可抑制DEX诱导的DNA片断化,其抑制作用具有剂量依赖性,以1g/L最明显;LBP(lg/L)尚可阻止DEX诱导的胸腺细胞内Ca ̄(2+)升高。结论:LBP可抑制DEX诱导的小鼠胸腺细胞凋亡,OXY对此无明显的调节作用。

Ginsenoside Rg1 is an active principle isolated from Panax ginseng CA Meyer. Primary neuronal culture was performed with different concentrations of Rg1. On the 14th day, the culture was changed to serum free medium. On the 16th day, neurons were harvested and assayed under microscope and fluorescence microscope. The result showed that serum withdrawal from the medium induced apoptosis in primary cultured cortical neurons. Rg1 (1, 10 μmol·L -1 ) was shown to inhibit apoptosis and protect neurons against...

Ginsenoside Rg1 is an active principle isolated from Panax ginseng CA Meyer. Primary neuronal culture was performed with different concentrations of Rg1. On the 14th day, the culture was changed to serum free medium. On the 16th day, neurons were harvested and assayed under microscope and fluorescence microscope. The result showed that serum withdrawal from the medium induced apoptosis in primary cultured cortical neurons. Rg1 (1, 10 μmol·L -1 ) was shown to inhibit apoptosis and protect neurons against injury. Membrane fluidity was measured using fluorescence spectrophotometer in five groups of cultured cortical neurons: control (complete medium), apoptosis (serum free medium), Rg1 0 1 μmol·L -1 , Rg1 1 μmol·L -1 and Rg1 10 μmol·L -1 . Neurons were isolated enzymatically and loaded with the fluorescent dye, DPH (1,6 diphenyl 1,3,5 hexatriene). Membrane fluidity in control neurons (η 1 2928±0 1426) was shown to be significantly higher than that in apoptosis cells (η 2 9277±0 2004). The membrane fluidity of neurons treated with Rg1 (0 1, 1, 10 μmol·L -1 ) increased significantly (η 2 9172±0 1604, 2 4731±0 2187, 1 6436±0 1483). These findings indicate a substantial alteration of membrane fluidity with neuronal apoptosis. Increment of membrane fluidity provides an aspect of elucidating the mechanism of Rg1′s anti apoptosis function.

研究人参皂甙Rg1对神经细胞凋亡的抑制作用。用原代培养的大鼠脑皮层神经细胞为实验模型。结果表明,Rg1能增强细胞活性,降低LDH的释放,减轻细胞核形态的改变,减少DNA断裂,增加细胞膜流动性,抑制细胞凋亡。提示细胞凋亡与细胞膜流动性关系密切;Rg1的抗衰老作用与抗凋亡作用有关。

Using techniques of cell culture in vitro and flow eytometry,the cellcycle distribution, cell proliferation index and gene expression of c-myc,c-fos and bc1-2of HL-60 cells of leukemia patients treated by Tanshinone (Tan,a Chinese herbal extract) were analysed and compared with all-trans-retinoic acid (RA). The results showed that HL-60 cells tended to differentiate to granulocytes and that some cells(11-8% ) underwent apoptosis when treated with Tan at 0. 5μg/ml-1. Tan interferedwith cells entrance into S phase...

Using techniques of cell culture in vitro and flow eytometry,the cellcycle distribution, cell proliferation index and gene expression of c-myc,c-fos and bc1-2of HL-60 cells of leukemia patients treated by Tanshinone (Tan,a Chinese herbal extract) were analysed and compared with all-trans-retinoic acid (RA). The results showed that HL-60 cells tended to differentiate to granulocytes and that some cells(11-8% ) underwent apoptosis when treated with Tan at 0. 5μg/ml-1. Tan interferedwith cells entrance into S phase whereupon DNA synthesis of the cells was inhibited.The action of Tan at the molecular level was associated with high expression of c-fosgene and low expression of c-myc as well as bc1-2 gene. Much yet remains to be investigated about the relationship between the induced differentiation and apoptosis of thecells.

应用体外细胞培养及流式细胞仪技术,分析了丹参酮作用前后HL-60细胞的细胞周期分布、细胞增殖指数、p53、c-myc、c-fos及bc1-2基因产物的表达变化,并以全反式维甲酸为对照。结果表明:0.5μg.ml-1的丹参酮作用后,HL-60细胞趋向粒细胞系统分化,有部分细胞发生凋亡(11.8%),丹参酮通过阻止HL-60细胞进入S期而抑制其DNA合成,其作用的分子机制与c-fos基因的表达增高、c-myc基因及bc1-2基因的表达降低有关,诱导分化与细胞凋亡的关系尚有待进一步探讨。

 
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