Method: SABC immunohistochemical method and in situ hybridization method were used to investigate the expression of P16 protein and mRNA in 5 cases of normal salivary gland tissues and 64 cases of salivary gland tumors.
The expression of mRNA of c-fos gene and brain-derived neurotrophic factor in each group was observed 1,3,7,14 days after spinal injury by in situ hybridization method and image quantitative analysis of Motic Images Advanced 3.0 RESULTS: Totally 72 Wistar rats entered result analysis.
Method The in situ hybridization technique was used to analyze the amounts of expression of 5-HT_ 1A receptor mRNA in order to explore the anti-depressive mechanism of the active components of Sini Powder.
In this paper a numerical simulation for intake water temperature and aquatic environment temperature field of cooling water system for the thermal power plant under the action of tide motion by using the hybrid method of fractional steps with L∞-stability is given, and its hydro-thermal behavior and environment pollution are predicted.
Methods The changes of apoptosis and bcl-2/bax gene expression were detected by DNA gel electrophoresis, flowcytometric analysis and immunohistochemistry, in situ hybridization methods in cholangiocarcinoma SK-ChA-1 cells after treated with SMS(100?ng/ml) for 6, 12 and 24 hours.
方法用DNA凝胶电泳、流式细胞仪检测AnnexinV标记凋亡细胞、免疫组化和原位杂交方法研究SMS(10 0ng/ml)处理 6、12和 2 4h后人胆管癌SK ChA 1细胞凋亡和bcl 2 /bax表达的变化。
Methods On the wound of eschar excision wounds of severe burn patients of large area burns,s-ADM or h-ADM were overlapped and grafted with ultrathin split thickness skin autografts(USTS),and then,the immunohistochemistry and in situ hybridization methods were used to detect the percentages(%) of CD3,CD4,CD8,IFN-γ and IL-4 positive cells,and the expression levels and significance of mRNA of IL-4 and IFN-γ.
The Detection of Cyanobacterial Cells by a Non-Fluorescent in situ Hybridization Method
wesenbergii NIER10068) were tested by a nonfluorescent in situ hybridization method using two specific horseradish peroxidase-labeled oligonucleotide probes and two chromogenic substrates.
A Study of the Composition of the Aquatic Bacterial Community of Lake Baikal by the in situ Hybridization Method
The possible application of the in situ hybridization method to detect soil microflora able to transform the herbicide atrazine was investigated.
By the example of a chimeric AML1-ETO sequence, a marker of frequently occurring leukemia, the express hybridization method was shown to allow the rapid identification of single molecules and the determination of titers of DNA and RNA targets.
A hybrid method for solving variational inequality problems
Analysis of airborne antenna using a FEM-UTD hybrid method
Hybrid method of combined difference and spectrum for time-domain maxwell's equations
Although yeast two-hybrid system is obviously advantageous in searching interacting proteins and setting up the network of proteins interaction, not all of proteins can use routine yeast two-hybrid method to search interacting proteins.
The numerical solution of Green's function for the potential in 2-D arbitrary inhomogeneous media with axial symmetry has been given by use of efficient half-analytical, halfnumerical hybrid method.