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杂交方法     
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  hybridization method
     The expression of cbfa1 mRNA was detected by in situ hybridization method.
     用原位杂交方法检测cbfa1mRNA的表达。
短句来源
     The expressions of STAT3, STAT5, LMP1, Survivin and Ki67 were detected with immunohistochemical method. The expression of EBER1/2 was detected with in-situ hybridization method.
     用免疫组化方法检测STAT3、STAT5、LMP1、Survivin和Ki67及用原位杂交方法检测EBER1/2在25例鼻NK/T细胞淋巴瘤中的表达。
短句来源
     Method: SABC immunohistochemical method and in situ hybridization method were used to investigate the expression of P16 protein and mRNA in 5 cases of normal salivary gland tissues and 64 cases of salivary gland tumors.
     方法 :应用免疫组化和原位杂交方法检测了 5例正常涎腺和 6 4例涎腺肿瘤标本中P16蛋白和P16mRNA的表达 ,并分析了P16蛋白和P16mRNA表达与患者临床病理参数的关系。
短句来源
     The expression of mRNA of c-fos gene and brain-derived neurotrophic factor in each group was observed 1,3,7,14 days after spinal injury by in situ hybridization method and image quantitative analysis of Motic Images Advanced 3.0 RESULTS: Totally 72 Wistar rats entered result analysis.
     应用原位杂交方法及MoticImagesAdvanced3.0图像定量分析法观察脊髓损伤后1,3,7,14d时各组c-fos基因mRNA和脑源性神经营养因子mRNA的表达变化。 结果:72只Wistar大鼠均进入结果分析。
短句来源
     Methods In situ hybridization method was used to detect CD44v6 mRNA and p16 mRNA expression in 46 cases of PTC.
     方法 应用原位杂交方法 ,检测 4 6例PTC组织中CD4 4v6和 p16的mRNA表达。
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  hybridization technique
     AT 1R genotypes(AA, AC and CC) and PAI 1 genotypes ( 4G/4G, 4G/5G and 5G/5G) were analyzed by the allele specific oligonucleotide hybridization technique.
     采用等位基因特异性寡核苷酸探针杂交方法分析 AT1 R基因型 (AA、AC和 CC)和 PAI- 1基因型 (4G/ 4G、4G/ 5 G和 5 G/ 5 G)。
短句来源
     Method The in situ hybridization technique was used to analyze the amounts of expression of 5-HT_ 1A receptor mRNA in order to explore the anti-depressive mechanism of the active components of Sini Powder.
     方法 采用原位杂交方法分析 5 HT1A 受体mRNA表达的数量 ,以探讨四逆散活性成分抗抑郁机制。
短句来源
     AT 1R and PAI-1 genotypes were analyzed by the allele specific oligonucleotide hybridization technique.
     采用等位基因特异性寡核苷酸探针杂交方法分析AT1R基因型和PAI - 1基因型。
短句来源
     ③ mRNA expressions of collagenase (MMP-1) and tissue inhibitor of metalloproteinase (TIMP-1) were measured with in situ hybridization technique.
     ③原位杂交方法观察胶原降解有关酶类:胶原酶(MMP-1)和金属蛋白酶组织抑制剂(TIMP-1)mRNA的表达。
短句来源
     Methods: Immunohistochemical technique and in situ hybridization technique were used to detect P16 protein, HBxAg and p16 mRNA.
     方法:采用免疫组化及原位杂交方法在87例肝细胞癌及其癌旁肝组织内检测P16蛋白、HBxAg及p16mRNA。
短句来源
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  hybrid method
     A New Hybrid Method of Crossovers and Its Application to Constrained Optimization
     一种新的混合杂交方法及其在约束优化中的应用
短句来源
     To speed up the method, we combine the structured MBFGS method with Gauss-Newton method to propose a hybrid method.
     并且在有限次迭代后,对于零残量问题,该杂交方法退化为Guass-Newton方法,对于非零残量问题,该杂交方法退化为结构化MBFGS方法。
短句来源
     In this paper a numerical simulation for intake water temperature and aquatic environment temperature field of cooling water system for the thermal power plant under the action of tide motion by using the hybrid method of fractional steps with L∞-stability is given, and its hydro-thermal behavior and environment pollution are predicted.
     本文采用L_∞稳定的分步杂交方法,数值模拟了热电厂冷却水系统在潮汐作用下的取水温度和水环境温度,对其水力热力特性和水环境污染进行了预测。
短句来源
     This bode introduces fusion of beer Yeast protoplasm hybrid method.
     介绍啤酒酵母原生质体融合的杂交方法
短句来源
     A HYBRID METHOD OF FRACTIONAL STEQS WITHI_∞STABILITY FOR NUMERICAL MODELLINGOF AQUATIC ENVIRONMENTS
     数值模拟水环境污染的一种L_∞稳定的分步杂交方法
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  hybridization methods
     ④Transforming growth factor-β1 (TGF-β1) and TGF-β1 mRNA expressions in lung tissues were detected by immunohistochemistry and in situ hybridization methods.
     ④采用免疫组织化学方法和原位杂交方法检测肺组织转化生长因子β1及转化生长因子β1mRNA的表达。
短句来源
     In situ hybridization methods were used to detect the expression of CD44v6 mRNA and nm23H1 mRNA in HCC.
     应用原位杂交方法 ,检测分析HCC组织中CD44v6mRNA和nm2 3HmRNA表达。
短句来源
     Methods The changes of apoptosis and bcl-2/bax gene expression were detected by DNA gel electrophoresis, flowcytometric analysis and immunohistochemistry, in situ hybridization methods in cholangiocarcinoma SK-ChA-1 cells after treated with SMS(100?ng/ml) for 6, 12 and 24 hours.
     方法用DNA凝胶电泳、流式细胞仪检测AnnexinV标记凋亡细胞、免疫组化和原位杂交方法研究SMS(10 0ng/ml)处理 6、12和 2 4h后人胆管癌SK ChA 1细胞凋亡和bcl 2 /bax表达的变化。
短句来源
     Methods:Southern and Norhtern hybridization methods were used to screen the cDNA subtractive library constructed from ATRA induced human lung adenocarcinoma cell line GLC 82 in our laboratory.
     方法 :采用Southern和Northern杂交方法对本室用ATRA诱导人肺腺癌细胞GLC 82 ,经消减杂交所构建的cDNA消减文库进一步鉴定 ;
短句来源
     Methods On the wound of eschar excision wounds of severe burn patients of large area burns,s-ADM or h-ADM were overlapped and grafted with ultrathin split thickness skin autografts(USTS),and then,the immunohistochemistry and in situ hybridization methods were used to detect the percentages(%) of CD3,CD4,CD8,IFN-γ and IL-4 positive cells,and the expression levels and significance of mRNA of IL-4 and IFN-γ.
     方法 在临床大面积烧伤患者深度切痂创面上 ,将s ADM或h ADM与超薄断层自体皮重叠移植 ,采用免疫组化和原位杂交方法检测移植物中CD3、CD4、CD8、γ 干扰素 (IFN γ)和白细胞介素 4 (IL 4 )阳性细胞的百分比 (% ) ,以及IFN γ和IL 4mRNA的表达水平。
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  hybridization method
The Detection of Cyanobacterial Cells by a Non-Fluorescent in situ Hybridization Method
      
wesenbergii NIER10068) were tested by a nonfluorescent in situ hybridization method using two specific horseradish peroxidase-labeled oligonucleotide probes and two chromogenic substrates.
      
A Study of the Composition of the Aquatic Bacterial Community of Lake Baikal by the in situ Hybridization Method
      
The possible application of the in situ hybridization method to detect soil microflora able to transform the herbicide atrazine was investigated.
      
By the example of a chimeric AML1-ETO sequence, a marker of frequently occurring leukemia, the express hybridization method was shown to allow the rapid identification of single molecules and the determination of titers of DNA and RNA targets.
      
更多          
  hybridization technique
Usingin situ hybridization technique, the expression ofNfbp6 was investigated in various stages of flower development.
      
Molecular cloning of rice cDNAs related to pollen development using the subtraction hybridization technique
      
By using the cDNA subtraction hybridization technique, three cDNA fragments,RP-1, RP-2 andRP-3 have been successfully cloned from Annong N.
      
The application of the chromosomalin situ hybridization technique on fish and the relationship between CR1 repetitive sequence distribution and its function have been discussed.
      
Our analysis indicates that the total DNA hybridization technique may be useful in preliminary studies of taxonomic relationships between species of small animals.
      
更多          
  hybrid method
A hybrid method for solving variational inequality problems
      
Analysis of airborne antenna using a FEM-UTD hybrid method
      
Hybrid method of combined difference and spectrum for time-domain maxwell's equations
      
Although yeast two-hybrid system is obviously advantageous in searching interacting proteins and setting up the network of proteins interaction, not all of proteins can use routine yeast two-hybrid method to search interacting proteins.
      
The numerical solution of Green's function for the potential in 2-D arbitrary inhomogeneous media with axial symmetry has been given by use of efficient half-analytical, halfnumerical hybrid method.
      
更多          
  hybridization methods
Detecting expression of MRP-1/CD9 mRNA in lung cancers using tissue microarrays and fluorescence in situ hybridization methods
      
The expression levels of caspase-3 protein and bcl-2 and fas mRNA in HCMV infected cells (MOI=0.25) at 72 h post-infection were detected by Western blot andin situ hybridization methods, respectively.
      
esculentum were analyzed by DNA hybridization methods.
      
Two different DNA hybridization methods were used to study the genetic relationships among strains of Chlorella sorokiniana.
      
Hybridization methods for the analysis of nonlinear systems
      
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