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酶供体
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  enzyme donor
     A novel homogeneous immunoassay system, cloned enzyme donor immunoassay system(CEDIA),has been developed by Henderson in 1986 on the basis of a -complementation reaction of 3 -galactosidase (E.coli) . In the CEDIA test, genetic engineering was used for the generation and selection of enzyme acceptor (EA) and enzyme donor (ED) of P -galactosidase.
     Henderson等人在1986年利用基因工程技术生产大肠杆菌β-半乳糖苷酶酶受体(EA)和酶供体(ED),并利用其α-互补功能创立了克隆酶供体免疫分析(CEDIA)技术。
短句来源
  “酶供体”译为未确定词的双语例句
     loned enzymer doner immunoassay (CEDIA) with automatic analyzer for detection of theophylline was used, it′s calibration curve presented a linear relationship in range of 0 to 40μg/ml.
     用克隆化酶供体免疫分析法(CEDIA)在全自动生化分析仪上测定茶碱的血药浓度,在0~40μg/ml范围内线性关系良好。
短句来源
     Determination of theophylline by Cloned enzymer doner immunoassay in comparison with HPLC
     用克隆化酶供体免疫分析法测定茶碱血药浓度
短句来源
     2. The protein obtained by this way was highly active a -complementation.
     2.我们制备的EA蛋白、ED蛋白能用于研究6-半乳糖昔酶的a一互补,为制备克隆酶供体免疫分析试剂打下了基础。
短句来源
     Objetive To compare CEDIA with RIA in determination of serum digoxin.
     目的克隆酶供体免疫分析技术(CEDIA)与放免法(RIA)测定血清地高辛的两种实验方法的观察比较。
短句来源
  相似匹配句对
     Enzyme mimics
     模型
短句来源
     It couldn’t express in T.
     但该在T .
短句来源
     Determination of theophylline by Cloned enzymer doner immunoassay in comparison with HPLC
     用克隆化供体免疫分析法测定茶碱血药浓度
短句来源
     The αALDC activity of recombinant bacterium was 10 000fold higher than that of Bacillus brevis.
     活检测表明重组的α-ALDC的活性是供体菌的10000倍。
短句来源
     coli harboring orfz gene was used as a provider of pTn5cat transposon vector and S.fredii was used as a acceptor.
     coli为供体菌,以S.
短句来源
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  enzyme donor
Enzyme subunit, called the enzyme donor, is used as a label on antigen.
      


2′-5′oligo(A) is an important mediator in the expression of interferon runction.In this paper the substrate specificity of T_4 RNA ligase and the possibility for thesynthesis of 2′-5′oligo(A) derivatives by T_4 RNA ligase were examined.It was found(1980) that 2′-5′oligo(A) can be used as acceptor for T_4 RNAligase.This problem was further studied here in our laboratories and it was shownthat 2′-5′P_3A_3 can be used as an acceptor in joining with different nucleoside diphos-phates(pNp,N=A,G,G,U);pCpUpC;pCpm_2~2G...

2′-5′oligo(A) is an important mediator in the expression of interferon runction.In this paper the substrate specificity of T_4 RNA ligase and the possibility for thesynthesis of 2′-5′oligo(A) derivatives by T_4 RNA ligase were examined.It was found(1980) that 2′-5′oligo(A) can be used as acceptor for T_4 RNAligase.This problem was further studied here in our laboratories and it was shownthat 2′-5′P_3A_3 can be used as an acceptor in joining with different nucleoside diphos-phates(pNp,N=A,G,G,U);pCpUpC;pCpm_2~2G etc.by T_4 RNA ligase.The opinion was incurred that oligonucleotides with the 2′-5′phosphodiester bondcan scarcely be used as a donor for T_4 RNA ligase.Evidence provided here thatpA~(2′)p~(5′)A can join with CpUpC,UpCpCpA,Cpm~1IpψpG by the action of T_4 RNA ligasesuggests that oligo(A) with the 2′-5′ phosphodiester bond can be used as a donor forT_4 RNA ligase.Using T_4 RNA ligase,oligonucleotides containing the 2′-5′phosphodiester bondas well as the 3′-5′phosphodiester bond can be synthesized.Evidence was also obtainedto show that A~(2′)p~(5′) A can be used as the substrate of T_4 polynucleotido kinase.

在干扰素的功能表达中,2′-5′寡聚腺苷酸是一类重要的媒介物。本文研究了T_4-RNA连接酶的专一性及其用于2′-5′寡聚腺苷酸衍生物合成的可能性。1980年,我们曾发现2′-5′寡聚腺苷酸可以作为RNA连接酶的受体。本文对此作了进一步的研究,证实了T_4-RNA连接酶可以将pNp(N=A,G,C,U)、pCpUpC、pCpm_2~2G等供体连到2′-5′P_3A_3受体上去,生成各种相应产物。2′-5′磷酸二酯键连接的寡核苷酸能否作为T_4-RNA连接酶的供体,有人估计不大可能。本文也证实了T_4-RNA连接酶能将供体pA~(2′)p~(5′)A连接到CpUpC、UpCpCpA、Cpm′IpψpG等受体上面去。从而说明T_4-RNA连接酶也可使用2′-5′磷酸二酯键连接的寡核苷酸作为供体。应用T_4-RNA连接酶,可以合成既含有2′-5′又含有3′-5′磷酸二酯键的寡核苷酸。本工作还证明A~(2′)p~(5′)A也可以作为T_4-多核苷酸激酶的底物。

loned enzymer doner immunoassay (CEDIA) with automatic analyzer for detection of theophylline was used, it′s calibration curve presented a linear relationship in range of 0 to 40μg/ml.In this paper, the results of 63 samples correlated favorably with HPLC ( r = 0.987 , a = 1.025 ). The pharmacokinetic results of six patients by two methods showed no significant difference. The CEDIA is suitable for detection of the theophylline in plasma as a routine.

用克隆化酶供体免疫分析法(CEDIA)在全自动生化分析仪上测定茶碱的血药浓度,在0~40μg/ml范围内线性关系良好。和高效液相色谱法(HPLC)比较,63例样品相关系数为0.987,斜率为1.025,且对6例病人的茶碱药动学研究两法亦无明显差异。

Objetive To compare CEDIA with RIA in determination of serum digoxin. Method Digoxin concentration in 206 patients with heart disease was determined by the method of CEDIA and comared with that of RIA. Results Statistical analysis showed that the concentrations of digoxin of 151 cases in the non - intoxicated group and 55 cases in the intoxicated group sera were 1. 23 ± 0. 46ng/ml and 2. 59 ± 0. 62ng/ml respectively, approximating those repeted abroad. Conclusion CEDIA has a remarkably good correlation with...

Objetive To compare CEDIA with RIA in determination of serum digoxin. Method Digoxin concentration in 206 patients with heart disease was determined by the method of CEDIA and comared with that of RIA. Results Statistical analysis showed that the concentrations of digoxin of 151 cases in the non - intoxicated group and 55 cases in the intoxicated group sera were 1. 23 ± 0. 46ng/ml and 2. 59 ± 0. 62ng/ml respectively, approximating those repeted abroad. Conclusion CEDIA has a remarkably good correlation with RIA(Radioimmunoassay), r= 0. 9263.

目的克隆酶供体免疫分析技术(CEDIA)与放免法(RIA)测定血清地高辛的两种实验方法的观察比较。方法采用CEDIA对206例心脏疾病患者的血清地高辛浓度进行测定。结果经统计学分析,151例非中毒组和55例中毒组血清地高辛浓度分别为1.23±0.46ng/ml和2.59±0.62ng/ml。结论实验结果与国外报道接近,与放射免疫分析进行相关实验,结果r=0.9263,相关良好。

 
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