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酶表达
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  “酶表达”译为未确定词的双语例句
    Expression of insulin degrading enzyme in insulin resistance cell model
    胰岛素抵抗细胞模型的胰岛素降解酶表达
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    The growth and enzymes expression of the thymuses of newborn mice in vitro
    新生小鼠胸腺的体外生长及酶表达
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    Activated Lymphocytes Induces the Expression of Matrix Metalloproteinase in Cultured Human Vascular Smooth Muscle Cells
    活化淋巴细胞诱导人血管平滑肌细胞基质金属蛋白酶表达
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    Effect of Oxidized Low Density Lipoprotein on the Expression and Activity of Matrix Metalloproteinases in Human Monocytes
    氧化型低密度脂蛋白对人血单核细胞基质金属蛋白酶表达及活性的影响
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    Relationship between Expression of AmpC Beta-lactamase in Enterobacter Cloacae and ampD-ampR Regulatory Gene
    ampD-ampR调节基因与阴沟肠杆菌AmpC酶表达关系的研究
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  enzyme expression
Inhibitors of COX-2 efficiently suppressed oxidative stress and enzyme expression in the cells treated with Cum-OOH.
      
Tryptophan 7-Halogenase from Pseudomonas aureofaciens ACN Strain: Gene Cloning and Sequencing and the Enzyme Expression
      
Many foodstuffs act as chemopreventers by altering xenobiotic metabolising enzyme expression in favour of detoxication over bioactivation pathways.
      
However, IQ itself can also affect enzyme expression, which may be a confounding factor in chemoprevention studies.
      
Enzyme expression and activity were determined by Western blotting and the use of selective probe substrates as appropriate.
      
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  enzyme expression
Inhibitors of COX-2 efficiently suppressed oxidative stress and enzyme expression in the cells treated with Cum-OOH.
      
Tryptophan 7-Halogenase from Pseudomonas aureofaciens ACN Strain: Gene Cloning and Sequencing and the Enzyme Expression
      
Many foodstuffs act as chemopreventers by altering xenobiotic metabolising enzyme expression in favour of detoxication over bioactivation pathways.
      
However, IQ itself can also affect enzyme expression, which may be a confounding factor in chemoprevention studies.
      
Enzyme expression and activity were determined by Western blotting and the use of selective probe substrates as appropriate.
      
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With PCR point mutation technique,the CCAAC box in the promoter region of δ-globingene was changed into CCAAT which is required in the normal transcription of β-globin geneat the corresponding site.The normal and mutant segments of the δ-globin promoter regionobtained with each segment of about 300 bp(-280/+54 bp),a fragment sufficient for therequirement of its normal promoter function,were separately cloned into pUC19/Hinc Ⅱ.After sequencing,it showed that the CCAAC box had been successfully changed intoCCAAT...

With PCR point mutation technique,the CCAAC box in the promoter region of δ-globingene was changed into CCAAT which is required in the normal transcription of β-globin geneat the corresponding site.The normal and mutant segments of the δ-globin promoter regionobtained with each segment of about 300 bp(-280/+54 bp),a fragment sufficient for therequirement of its normal promoter function,were separately cloned into pUC19/Hinc Ⅱ.After sequencing,it showed that the CCAAC box had been successfully changed intoCCAAT without other mutations. The two segments were released by Nco Ⅰ and BamH Ⅰ,and were cloned into the corresponding site of plasmid pMG3 which has the firefly luciferaseas its reporter gene.The positive clones were selected by Dot Blot and were comfirmed withrestriction mapping. Then the two recombinant plasmids were transfected into HeLa cell linerespectively and the promoter activities of the two fragn1ents were identified by comparingthe light intensity generated by the cell extracts when added with luciferase assay substrate.The results demonstrated that the luminescence of the mutant group is on average five timeshigher than that of the normal group,which indicate that the C-T mutation of the CCAACbox of δ-globin promoter region could increase its promoter activity. Our results have confirmed the hypothesis that the sequence of CCAAC box of human δ-globin gene is one of themain reasons which accounts for its low expression level.The author is now studying thespecific DNA binding protein of the upstream promoter region of human δ-globin gene.

以pUC19-δglobin为模板,用PCR定点突变技术将δ珠蛋白基因启动子区-64位的C突变为T,即将CCAAC盒改变为CCAAT,并获得野生及突变的δ珠蛋白基因启动子区约300bp的两个片段。将此两片段分别克隆到以虫荧光素酶为报告基因的表达载体PMG3中,转染人HeLa细胞,以瞬时表达分析突变的CCAAC盒的启动子功能。结果表明突变组发动荧光素酶表达而产生的发光强度为野生型的5倍,说明将δ珠蛋白基因启动子区-64位的CCAAC盒改变为CCAAT能够增强该启动子的功能,从而证实了δ基因启动子区CCAAC盒的结构特点是该基因表达水平低的主要原因之一。

Objective:To study the effect of IFN α on the expressions of perforin and granzymes in IL 2 activated lymphocytes.Methods:NK and LAK activities were assayed by 4 hour standard 51 Cr release test,the activity of perforin was detected by hemolysis method,expression of granzyme B was measured by ABC immunohistological method,expression of granzyme A was measured by BLT method.Results:IFN α significantly augmented the activities of NK and LAK in IL 2 activated peripheral blood lymphocytes(PBL) after...

Objective:To study the effect of IFN α on the expressions of perforin and granzymes in IL 2 activated lymphocytes.Methods:NK and LAK activities were assayed by 4 hour standard 51 Cr release test,the activity of perforin was detected by hemolysis method,expression of granzyme B was measured by ABC immunohistological method,expression of granzyme A was measured by BLT method.Results:IFN α significantly augmented the activities of NK and LAK in IL 2 activated peripheral blood lymphocytes(PBL) after 1 day culture.Perforin activity in lymphocytes was increased after 1 day exposure to IL 2 or IFN α,and was enhanced when exposed to the combination of IL 2 and IFN α.After 3 day culture,the perforin activity remained high in lymphocytes activated by IL 2 alone or in combination with IFN α,while declined to control level in IFN α exposed group.IL 2 and IFN α alone or in combination had no effect on expression of granzyme A and B.Conclusion:IFN α enhances the cytotoxicity of lymphocytes activated by IL 2.The mechanism might be that IFN α upregulates the perforin expression.

目的:研究α干扰素(IFN-α)对白细胞介素2(IL-2)激活的淋巴细胞中穿孔素和颗粒酶表达的影响。方法:4小时标准51Cr释放实验检测NK和LAK活性,ABC酶标法检测颗粒酶B的表达,溶血法测定穿孔素活性,BLT法测定颗粒酶A的表达。结果:IFN-α能增强IL-2诱导的外周血淋巴细胞中NK和LAK细胞活性。IL-2和IFN-α分别作用于淋巴细胞1天即可增加穿孔素活性,二者联合可使活性进一步增强(P<0.05),于培养第3天,IL-2和联合组的穿孔素仍有高表达,但IFN-α组则下降至对照组水平。IFN-α和IL-2单独或联合对淋巴细胞颗粒酶A和B的表达无影响。结论:IFN-α增加IL-2激活淋巴细胞的细胞毒作用可能与其增加淋巴细胞穿孔素表达相关。

Recombinant luciferase repoter genes(pSIS 1758/+43 Luc and pSIS 402/+43 Luc)containing different upstream sequences( 1758/+43bp and 402+43bp,respectively)of human PDGF B chain gene have been constructed.After these reporter genes and pSV β galatosidase vector were cotransfected into human vascular endothelial cells,the activities of the cellular luciferase and β galatosidase were measured under either normal state or TNFα treatment.The results suggest that under TNF a treatment,presence of upstream...

Recombinant luciferase repoter genes(pSIS 1758/+43 Luc and pSIS 402/+43 Luc)containing different upstream sequences( 1758/+43bp and 402+43bp,respectively)of human PDGF B chain gene have been constructed.After these reporter genes and pSV β galatosidase vector were cotransfected into human vascular endothelial cells,the activities of the cellular luciferase and β galatosidase were measured under either normal state or TNFα treatment.The results suggest that under TNF a treatment,presence of upstream sequences of PDGF B chain gene up regulates luciferase expression in endothelial cells and a TNFα induced positive regulatory element may be restricted to 1758/ 403bp.

本研究构建了含人PDGFB链基因不同上游序列的荧光素酶报告基因pSIS-1758/+43Luc和pSIS-402/+43Luc,经与pSVβGalactosidaseControlVector共转染血管内皮细胞后,测定了基础水平和TNFα作用下细胞裂解液中的荧光素酶和β半乳糖苷酶活性。结果表明:在TNFα作用下,PDGFB链基因上游序列上调内皮细胞荧光素酶的表达,其主要调控区可能位于-1758/-403bp之间。

 
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