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   斑点杂交试验 的翻译结果: 查询用时:0.019秒
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斑点杂交试验
相关语句
  spot hybridization test
     SERUM DIRECT SPOT HYBRIDIZATION TEST DETECTION OF HBV-DNA AND THE FACTORS INFLUNC1NG ITS SENSITIVITY AND SPECIFICITY
     血清直接斑点杂交试验检测HBV-DNA及其影响因素的探讨
短句来源
     This paper described a direct spot hybridization test using 5-10ul sera sample and various factors related its sensitivity and specificity were discussioned.
     本文介绍了一种只用5—10uL血清样本的直接斑点杂交试验
短句来源
  dot blot test
     The results of radio labelled assay and dot blot test showed the modifying enzyme patterns of aminoglycoside produced by 50 strains of MRSA were the same as which extrapolated form the resistant profiles.
     根据对50株MRSA的核素标记分析和斑点杂交试验获得的结果与其耐药谱推测的结果是一致的;
短句来源
  “斑点杂交试验”译为未确定词的双语例句
     Inject the mice during lactating period with recombinant plasmid p205C3LYZ and detect the expression of hLYZ cDNA in vivo of mice by micrococcal lysis assay and dot blot.
     重组载体p205C3LYZ注射哺乳期小鼠,用微球菌溶解试验和斑点杂交试验检测hLYZ cDNA在小鼠体内的表达。
短句来源
     cDNA fragments(P9-25)were labeled with PHOTOPROBE TM Biotin,and some of them were used as probes to detect target DNA by dot blot hybridization.
     cDNA片段(P9-25)等作为探针,并以斑点杂交试验检测靶DNA。
短句来源
     CLINICAL SIGNIFICANCE OF DIRECT SPOT HYBRIDIZATION IN SERUM HBV DNA
     斑点杂交试验直接检测血清HBV DNA的临床意义
短句来源
     DHBV in duck serum is assayed by α-~(32)P-DHBV DNA probe hybridization.
     用α-~(32)P-dATP-DHBV DNA探针做斑点杂交试验、检测了不同鸭种血清中携带DHBV情况。
短句来源
     322 clinical samples taken randomly from specific pathogen free (SPF) mice were submitted to detection by the dot blotting and previously established PCR and culture with a positive detection rate of 3.1% (10/322) and an agreement of 100 %.
     用斑点杂交试验和PCR对 32 2份SPF小鼠的临床样品进行了检测 ,检出率为 3 1% (10 /32 2 ) ,符合率为 10 0 % ,与细菌学检查的结果相一致。
短句来源
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  相似匹配句对
     Studies On Crossing Application of Shou Guang Chicken
     寿光鸡杂交试验
短句来源
     RNA dot hy- bridization.
     RNA斑点杂交
短句来源
     HYBRIDIZATION EXPERIMENTS WITH TWO SPECIES OF DENDROLIMUS
     松毛虫的杂交遗传试验
短句来源
     TESTING
     试验
短句来源
     CLINICAL SIGNIFICANCE OF DIRECT SPOT HYBRIDIZATION IN SERUM HBV DNA
     斑点杂交试验直接检测血清HBV DNA的临床意义
短句来源
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  spot hybridization test
The spot hybridization test detected PSTV in mixtures of seed extracts equivalent to as few as one seed from a PSTV-infected plant and 80 seeds from healthy plants.
      
The spot hybridization test was shown to be more sensitive and reliable than PAGE analysis; it is suitable for the testing of large numbers of samples with a minimum expenditure of labor and materials.
      
The dot-spot hybridization test was reproducible with formaldehyde-denaturated cell-sap samples stored at room temperature for over 6 months.
      
  dot blot test
A newly developed milk dot blot test was used to detect anti-bovine leukaemia virus (BLV) antibody in milk samples from 2079 lactating adult cows from among 61 herds.
      
The milk dot blot test was highly repeatable; the concordance rate, compared with the agar gel immunodiffusion test performed on serum, was 83.5%.
      
We used an immuno-dot blot test that detects the proteins using antibodies specific for actin or eEF1A.
      


Determination of serum HBV-DNA in 39 cases of chronic hepatitis was performed by means of direct spot hybridization assay. Of the 39 specimens 25 yielded positive results (64.1%)o 15 out of 23 with CAH (65.2%), 7 out of 10 cases with CPH (70%) 2 out of 5 with HLC (40%) and 1 case of SH were positive for HBV-DNA determination.

本文报导采用斑点杂交试验对39例慢性乙型病毒性肝炎的血清HBV-DNA检测结果。39例的阳性率为64.1%(25/39)。其中CAH阳性率为65.21%(15/23),CPH为70%(7/10),HLC为40%(2/5),SH1例为阳性。对HBV-DNA与乙型肝炎病毒血清标志的相关性作了初步分析。

One hundred and one patients with HBV asymptomatic carrier, chronic active hepatitis (CAN) and liver cirrhosis were assayed for HBV DNA with direct spot hydridization. HBV DNA positive rates were58.3% (21/36), 39.6% (17/46) and 10.5% (2/21), respectively. There was significant difference among three groups (p<0.01). HBV DNA positive rates were 95.2% (20/21), 70.8% (17/24) and 25.0% (1/4) , respectively, when HBeAg was positive. There was significant difference among three groups (p<0.05). It is not accordant...

One hundred and one patients with HBV asymptomatic carrier, chronic active hepatitis (CAN) and liver cirrhosis were assayed for HBV DNA with direct spot hydridization. HBV DNA positive rates were58.3% (21/36), 39.6% (17/46) and 10.5% (2/21), respectively. There was significant difference among three groups (p<0.01). HBV DNA positive rates were 95.2% (20/21), 70.8% (17/24) and 25.0% (1/4) , respectively, when HBeAg was positive. There was significant difference among three groups (p<0.05). It is not accordant that HBsAg, anti-HBc and anti-HBe were compared with serum HBV DNA. These results indicate that HBV asymptomatic carriers are more infectious than CAH and liver cirrhosis. They are extremely infectious sources.

本文应用斑点杂交试验直接检测101例乙肝病毒(HBV)无症状携带者、慢活肝及肝硬化患者血清HBV DNA,阳性率分别为58.3%(21/36)、36.9%(17/46)、10.5%(2/21),三组间有显著差异(P<0.01)。并与血清免疫标志进行比较,在HBeAg阳性时,HBV DNA检出率分别为95.2%(20/21)、70.8%(17/24)、25.0%(1/4),三组间有显著差异(P<0.05)。HBsAg、抗-HBc及抗-HBe与血清HBV DNA并非一致。结果表明,HBV无症状携带者比慢活肝及肝硬化具有更高的传染性,是极重要的传染源。

Type I heat-labile toxin(LT1) DNA probes were labeled with digoxigenin, biotin, and 32P by random priming. Digoxigenin labeled, biotinylated, and 32P LTj probe detected 0.1 Pg, 1 Pg. and 0.1 Pg of EWD299 DNA in DNA blot hybridization; 12, 120 and 12 bacteria per millilitre of normal saline in bacteria in situ hybridization; 24, 240, and 24 bacteria per millilitre of stool suspension in stool blot hybridization after exposure to indicator dyes for 4 hours or X-ray film for 72 hours. The treatment of nitrocellulose...

Type I heat-labile toxin(LT1) DNA probes were labeled with digoxigenin, biotin, and 32P by random priming. Digoxigenin labeled, biotinylated, and 32P LTj probe detected 0.1 Pg, 1 Pg. and 0.1 Pg of EWD299 DNA in DNA blot hybridization; 12, 120 and 12 bacteria per millilitre of normal saline in bacteria in situ hybridization; 24, 240, and 24 bacteria per millilitre of stool suspension in stool blot hybridization after exposure to indicator dyes for 4 hours or X-ray film for 72 hours. The treatment of nitrocellulose membrane filters with RNase A and proteinase K eliminated the possibility of false-positivity and made the specificity of digoxigenin labeled and biotinylated LT: probes as good as 32P

采用随机引物法制备地高辛素、生物素和~(32)P标记产毒性大肠杆菌Ⅰ型不耐热肠毒素(LT_1)DNA探针。地高辛素、生物素和~(32)P LT_1探针分别在DNA斑点杂交试验中检测到0.1pg、1pg和0.1pg的质粒DNA,在菌落原位杂交试验中检测到12、120和12个细菌/ml,在粪便斑点杂交试验中检测到24、240和24个细菌/ml。采用RNA酶A和蛋白酶K消化处理滤膜,消除了假阳性,使得地高辛素、生物素LT_1探针的特异性与~(32)PLT_1探针完全一致。检测143株自腹泻病人分离的大肠杆菌,14株(9.79%)阳性,3种探针结果相同。

 
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