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化学吸引
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     Objective To establish MCP-1 siRNA human renal tubular epithelial cells line and to investigate the inhibitory effect of plasma-mediated small interfering RNA (siRNA) on the expression of monocyte chemoattractant protein-1 (MCP-1) gene in human renal tubular epithelial cells (HKC).
     目的建立单核细胞化学吸引蛋白质1(MCP-1)小分子干扰RNA(siRNA)人肾小管上皮细胞株,并检测MCP-1 siRNA对人肾小管上皮细胞(HKC)MCP-1基因的抑制效应。
短句来源
     Establishment of monocyte chemoattractant protein-1 small interfering RNA human renal tubular epithelial cells line
     单核细胞化学吸引蛋白质1小分子干扰RNA人肾小管上皮细胞株的建立
短句来源
     In situ hybridization and immunochemistry were used to observe MCP 1 and M CSF expression in experimental renal tissue.
     原位杂交、免疫组化观察单核细胞化学吸引蛋白质 1(MCP 1)、巨噬细胞集落刺激因子 (M CSF)在肾内的表达分布。
短句来源
     Methods The concentration of MCP-1 in the cultured supernatants of HaCaT cell treated with tacrolimus was determined by ELISA. The expression level of MCP-1 mRNA in HaCaT cell treated with tacrolimus was studied by RT-PCR method.
     方法用不同浓度的他克莫司处理人角质形成细胞株HaCaT,24h后收集上清液,通过ELISA法检测上清液中的单核细胞化学吸引蛋白质1(monocytechemoattractantprotein1,MCP-1)浓度,并通过RT-PCR法检测细胞中MCP-1mRNA水平。
短句来源
     Objective To detect the expression of MCP-1 in psoriatic lesional keratinocytes and to investigate the mechanisms of VitD 3 in the treatment on psoriasis.
     目的 观察维生素D3 对角质形成细胞单核细胞化学吸引蛋白质 1(monocytechemoattractantprotein 1,MCP 1)的表达影响 ,探讨其在治疗银屑病中的作用机制。
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  相似匹配句对
     Radioanalytical Chemistry
     放射分析化学
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     The Rules of Attraction
     吸引的规律
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     CHAPTER 5 MARINE SEDIMENTATION Section 5 Sedimentary Chemistry
     沉积物化学
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     Attraction,Exchange and Development
     吸引·交流·发展
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  chemical attraction
The thermally pressed nylon/polyester fabric films showed a chemical attraction to formamide.
      
The sorption properties of the microfiber fabrics were investigated using a real time testing device, and these tests showed that the sorption behavior was more influenced by the structure of the fibrous assembly than by any chemical attraction.
      
Older pods less than 7 weeks before ripening (WBR) were preferred, suggesting greater nutritional value or chemical attraction of the older pods.
      
Chemical attraction to a filtrate of chironomids was significantly reduced after the 4-h exposure to 1 μg/L carbofuran.
      
Chemical attraction of the eastern yellowjacket,Vespula maculifrons (Hymenoptera: Vespidae)
      
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In this paper, the writer believes that the principal forms of motion of nature are mechanical, thermal, electro-magnetic, nuclear, fundamental particle, chemical and organic life motion. After analyzing the physical nature of attraction and repulsion in the world of non-living things, he points out that there exist biochemical attraction and repulsion in organic life motion. In conclusion, the writer proposes that attraction and repulsion be considered a contradiction common to all forms of motion of nature....

In this paper, the writer believes that the principal forms of motion of nature are mechanical, thermal, electro-magnetic, nuclear, fundamental particle, chemical and organic life motion. After analyzing the physical nature of attraction and repulsion in the world of non-living things, he points out that there exist biochemical attraction and repulsion in organic life motion. In conclusion, the writer proposes that attraction and repulsion be considered a contradiction common to all forms of motion of nature.

1、认为自然界的主要运动形式有:机械运动、热运动、电磁运动、核运动、基本粒子运动、化学运动和生命运动.2、讨论了非生物界中吸引和排斥的物理本质.3、指出在生命运动中存在着生物化学吸引和排斥作用.4、提出把吸引和排斥看成是自然界一切运动的共同矛盾.

Objective: To study the relationship between monocyte/macrophage(MC/MP) accumulation and tubulointerstitial fibrosis.Methods: The renal tubulointerstitial fibrosis model in Wistar rats was established by unilateral renal vein ligature. The rats were fed for 25 days. The kidneys were obtained every 5 days by killing the rats. The morphological changes of tubulointerstitial fibrosis were observed by light microscopy with HE,PAS, PASM and Masson staining. Immunohistochemistry and double immunohistochemistry...

Objective: To study the relationship between monocyte/macrophage(MC/MP) accumulation and tubulointerstitial fibrosis.Methods: The renal tubulointerstitial fibrosis model in Wistar rats was established by unilateral renal vein ligature. The rats were fed for 25 days. The kidneys were obtained every 5 days by killing the rats. The morphological changes of tubulointerstitial fibrosis were observed by light microscopy with HE,PAS, PASM and Masson staining. Immunohistochemistry and double immunohistochemistry were used to observe MC/MP accumulation and proliferation. In situ hybridization and immunochemistry were used to observe MCP 1 and M CSF expression in experimental renal tissue. The MCP 1 protein expression was inspected by Western blot. All the data were analyzed statistically.Results: The pathological changes of tubulointerstitial fibrosis were typical. There were many MC/MPs accumul sated in the interstitial space at the early stage and some of them were PCNA positive. At the late stage both accumulation and proliferation of MC/MPs were decreased. The portion of monocyte proliferation was high correlated with the MC/MP accumulation. In situ hybridization showed the positive signals of MCP 1 and M CSF were mainly located in the cytoplasm of degenerated tubular epithelium and they were strong at the early stage, weak at the late stage. MCP 1 by immunochemistry and Western blot were consistent with in situ hybridization. The MC/MP accumulation was high correlated with the expression of MCP 1 and tubular epithelium degeneration. The portion of monocyte proliferation was high correlated with the expression of M CSF.Conclusion:There was obvious accumulation of MC/MP at the early stage of tubulointerstitial fibrosis. The accumulation came from infiltration and proliferation which were regulated by degenerated tubular epithelial cells producing MCP 1 and M CSF. MC/MP accumulation was highly correlated with tubular degeneration. MC/MP promoted tubulointerstitial fibrosis and damaged tubular epithelium by secreting a variety of cytokines.

目的 :探讨肾小管间质纤维化与单核巨噬细胞 (MC/MP)的关系。方法 :结扎大鼠一侧肾静脉 ,连续饲养2 5d ,制作大鼠肾小管间质纤维化模型。每 5天杀检取材。HE、PAS、PASM、Masson等染色方法观察MC/MP的聚集和肾小管间质纤维化病变 ;免疫组化和双重免疫组化观察肾间质MC/MP的聚集与增生 ;原位杂交、免疫组化观察单核细胞化学吸引蛋白质 1(MCP 1)、巨噬细胞集落刺激因子 (M CSF)在肾内的表达分布。Westernblot检测病变肾脏MCP 1的表达。对不同时间点肾小管变性、肾小管间质纤维化程度、肾间质MC/MP聚集的数量及其中的增生比例、MCP 1和M CSF的表达进行评估分析。结果 :肾静脉结扎侧表现为肾小管变性萎缩、间质中MC/MP浸润、细胞外基质沉积等肾小管间质纤维化的典型病变。病变早期肾间质中有明显的MC/MP聚集 ,后期减少。表达增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)的单核细胞占一定比例 ,单核细胞的增生比例与聚集高度相关。原位杂交、免疫组化和Westernblot显示 ,病变肾组织中MCP 1、M C...

目的 :探讨肾小管间质纤维化与单核巨噬细胞 (MC/MP)的关系。方法 :结扎大鼠一侧肾静脉 ,连续饲养2 5d ,制作大鼠肾小管间质纤维化模型。每 5天杀检取材。HE、PAS、PASM、Masson等染色方法观察MC/MP的聚集和肾小管间质纤维化病变 ;免疫组化和双重免疫组化观察肾间质MC/MP的聚集与增生 ;原位杂交、免疫组化观察单核细胞化学吸引蛋白质 1(MCP 1)、巨噬细胞集落刺激因子 (M CSF)在肾内的表达分布。Westernblot检测病变肾脏MCP 1的表达。对不同时间点肾小管变性、肾小管间质纤维化程度、肾间质MC/MP聚集的数量及其中的增生比例、MCP 1和M CSF的表达进行评估分析。结果 :肾静脉结扎侧表现为肾小管变性萎缩、间质中MC/MP浸润、细胞外基质沉积等肾小管间质纤维化的典型病变。病变早期肾间质中有明显的MC/MP聚集 ,后期减少。表达增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)的单核细胞占一定比例 ,单核细胞的增生比例与聚集高度相关。原位杂交、免疫组化和Westernblot显示 ,病变肾组织中MCP 1、M CSFmRNA的表达主要位于变性的肾小管上皮细胞胞浆中 ,病变早期强 ,后期减弱。肾小管上皮细胞MCP 1和M CSF的表达与肾间质MC/MP的聚集高度相关。肾间质MC/MP的聚集与肾小管变性高度相关。结论 :肾小管间质?

Objective To detect the expression of MCP-1 in psoriatic lesional keratinocytes and to investigate the mechanisms of VitD 3 in the treatment on psoriasis.Methods The concentration of MCP-1 in the cultured supernatants of HACAT treated with VitD 3 was determined by ELISA. The expression level of MCP-1 mRNA in HACAT treated with VitD 3 was detected by RT-PCR.Results The concentration of MCP-1 in HACAT treated with proper concentrations of VitD3 was significantly decreased. The expression level of MCP-1mRNA...

Objective To detect the expression of MCP-1 in psoriatic lesional keratinocytes and to investigate the mechanisms of VitD 3 in the treatment on psoriasis.Methods The concentration of MCP-1 in the cultured supernatants of HACAT treated with VitD 3 was determined by ELISA. The expression level of MCP-1 mRNA in HACAT treated with VitD 3 was detected by RT-PCR.Results The concentration of MCP-1 in HACAT treated with proper concentrations of VitD3 was significantly decreased. The expression level of MCP-1mRNA was also markedly decreased.Conclusion The results demonstrated that VitD 3 might inhibit the expression of MCP-1 in keratinocytes and then suppress the chemotactic ability of monocytes and macrophages so as to prevent from some of inflammatory reactions.

目的 观察维生素D3 对角质形成细胞单核细胞化学吸引蛋白质 1(monocytechemoattractantprotein 1,MCP 1)的表达影响 ,探讨其在治疗银屑病中的作用机制。方法 培养角质形成细胞株HACAT ,并用不同浓度的维生素D3 处理 2 4h ,通过酶联免疫吸附 (ELISA)法检测上清液中MCP 1的表达 ;通过RT PCR法进一步检测细胞中MCP 1mRNA的水平。结果 经过浓度为 7.8× 10 -12 mol/L~ 6.2 5× 10 -11mol/L的维生素D3 处理过的角质形成细胞株HA CAT ,其分泌至上清中的MCP 1水平明显受到抑制 ,细胞中MCP 1mRNA的水平低于正常对照组。结论 维生素D3 在治疗银屑病过程中很有可能是通过抑制了角质形成细胞中MCP 1的表达 ,从而减弱了银屑病患者角质形成细胞趋化单核细胞及巨噬细胞的能力 ,阻止了银屑病皮损局部的炎症反应。

 
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