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   gus酶活 的翻译结果: 查询用时:0.526秒
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gus酶活
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  “gus酶活”译为未确定词的双语例句
     To study the regulation of hrp gene cluster of Xcc, the nonpolar hrpG integration mutants upon those hrp mutant strains, which Tn5 was inserted in ORF transcription direction, were generated. The GUS activities were mensurated in the plant-induced medium XVM2 before and after the hrpG mutagenesis.
     为研究hrp基因簇的调控,构建了hrp基因Tn5正向插入突变体的hrpG双突变体,在植物诱导培养基XVM2上检测hrpG突变前后GUS酶活变化。
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     semitecum was capable to produce some higher Cellulase activity than R.
     stolonifer产的高。
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     Preliminary probe into the influential factors of cellulase activity
     纤维素的影响因子初探
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     Enzyme mimics
     模型
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     LIVING IN MILAN
     在米兰
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     3) flexible.
     3)
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  gus activity
Promoter activity was estimated from quantification of β-glucuronidase (GUS) activity.
      
GUS activity ofAgrobacterium-mediated transgenic tobacco was measured.
      
Average GUS activity of the complementary sense promoter was 5-6 times that of CaMV 35S promoter, and the highest GUS activity of individual plant was ten times of that of CaMV 35S promoter.
      
Analysis of GUS activity of the transformants revealed that thePglaA activity of the strain T21 is about 3 times stronger than that of the strain AS 3.795.
      
Quantitative GUS assay showed that the average level of uidA expression was increased twofold for the presence of MAR, and the highest level of GUS activity of transgenic plants could be increased six times.
      
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Commelina Yellow Mottle Virus(CoYMV) is a double stranded, circular DNA virus and its promoter could direct GUS gene specifically expressing in phloem tissue of transgenic tobacco plants. To determine the optimal promoter sequence for phloem specific gene expression, CoYMV promoter was deleted from its 5′ end to form promoter fragments with 5 different lengths. Chimeric GUS genes were constructed using the promoter deletion based on the binary vector pBI121. Transgenic tobacco plants evidenced by PCR analysis...

Commelina Yellow Mottle Virus(CoYMV) is a double stranded, circular DNA virus and its promoter could direct GUS gene specifically expressing in phloem tissue of transgenic tobacco plants. To determine the optimal promoter sequence for phloem specific gene expression, CoYMV promoter was deleted from its 5′ end to form promoter fragments with 5 different lengths. Chimeric GUS genes were constructed using the promoter deletion based on the binary vector pBI121. Transgenic tobacco plants evidenced by PCR analysis were obtained with each kind of chimeric GUS gene structure by Agrobacterium mediated transformation. The results of GUS activity assay and histochemical staining showed that most of the chimeric GUS genes were expressed in transgenic plants. The GUS activity with the promoter deleted to -870bp was about 78% higher than that of the full length promoter(1040bp) and was a little higher than that of the promoter deleted to -585bp, but the difference is not significant. The GUS activity reduced significantly when the promoter was deleted to -447bp or -232bp, whereas the property of phloem specific expression pattern was still retained. When the promoter was deleted to -44bp, just upstream adjacent to the TATA box, its tissue specificity was lost and the activity was reduced to undetectable level.These results suggest that the region between -870bp~232bp and downstream of -232bp of CoYMV promoter could be responsible for promoter activity and tissue specific expression, respectively. A negative regulation sequence might exist upstream of -870bp of the CoYMV promoter. Therefore, we recommend that the optional CoYMV promoter sequence for phloem specific expression could be downstream from -870bp or -585bp. In comparison with CaMV 35S promoter, the GUS activity when driven by -870bp CoYMV promoter was about 70% of that when driven by the 35S promoter. Considering the fact that 35S promoter_GUS gene is constitutively expressed, while the CoYMV promoter GUS gene is expressed only in phloem tissues, the activity of the latter in phloem may be the same with or even higher than that of the 35S promoter.

竹节花黄斑驳病毒(CoYMV)是侵染单子叶植物竹节花的一种双链环状DNA病毒,它的启动子可介导外源基因在烟草韧皮部特异表达。为了研究其组织特异性表达的最佳启动子区域,对CoYMV启动子进行了5′端五种不同长度的缺失分析,用不同长度的启动子片段与GUS基因及NOS3′端转录中止序列构建了全长启动子及5个缺失启动子序列的六个嵌合GUS基因植物表达载体。利用农杆菌将上述嵌合基因转化烟草外植体后,每种表达载体都获得了一批转基因烟草植株。转化再生烟草植株的PCR分析、GUS酶活测定及GUS组织染色的结果表明六种类型的嵌合基因已整合到烟草染色体中,并有五种表达出GUS活性。缺失到870bp的启动子比全长启动子(1040bp)的活性约高78%,870bp比585bp启动子介导的GUS活性略高但差别不明显,缺失到447和232时GUS活性有明显下降,但仍具有韧皮部特异表达的特性。当缺失到TATAbox附近的44bp时启动子丧失组织特异性,GUS活性也降低到测不出来的水平。以上结果表明CoYMV启动子从转录起始位点上游870bp~230bp及232bp下游区分别与启动子的活性和韧皮部组织特异性密切相...

竹节花黄斑驳病毒(CoYMV)是侵染单子叶植物竹节花的一种双链环状DNA病毒,它的启动子可介导外源基因在烟草韧皮部特异表达。为了研究其组织特异性表达的最佳启动子区域,对CoYMV启动子进行了5′端五种不同长度的缺失分析,用不同长度的启动子片段与GUS基因及NOS3′端转录中止序列构建了全长启动子及5个缺失启动子序列的六个嵌合GUS基因植物表达载体。利用农杆菌将上述嵌合基因转化烟草外植体后,每种表达载体都获得了一批转基因烟草植株。转化再生烟草植株的PCR分析、GUS酶活测定及GUS组织染色的结果表明六种类型的嵌合基因已整合到烟草染色体中,并有五种表达出GUS活性。缺失到870bp的启动子比全长启动子(1040bp)的活性约高78%,870bp比585bp启动子介导的GUS活性略高但差别不明显,缺失到447和232时GUS活性有明显下降,但仍具有韧皮部特异表达的特性。当缺失到TATAbox附近的44bp时启动子丧失组织特异性,GUS活性也降低到测不出来的水平。以上结果表明CoYMV启动子从转录起始位点上游870bp~230bp及232bp下游区分别与启动子的活性和韧皮部组织特异性密切相关,87?

 
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