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多价基因
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  multiple genes
     Based on present plant gene engineering techniques, some strategies to develop transgenic crops expressing multiple genes by constr ucting a multigenes expression vector or a number of expression vectors and then making one or more transformations or co-transformation are discussed and the p rospects are described as well in this paper.
     本文探讨了在利用植物基因工程技术的基础上,通过构建多基因或者多个表达载体,进行一次、多次基因转化或共转化方法获得转多价基因作物的一些策略,并进行了展望。
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  “多价基因”译为未确定词的双语例句
     cry GENE ANALYSIS AND MULTI cry GENE COMBINATIONS OF SEVERAL HIGHLY TOXIC BACILLUS THURINGIENSIS STRAINS
     苏云金芽孢杆菌高效菌株cry基因分析及多价基因组合的研究
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     Construction of multivalent plant express vector of chi,rip and DREB1A gene
     rip、chi及DREB1A多价基因植物表达载体的构建
短句来源
     The main results were summarized as follows: (1) Construction of plant expression vectors One multivalent plant express vectors pBDCR was constructed, including that ribosome inactivating protein (rip) gene、chitinase (chi) gene and DREB1A gene, which were regulated respectively by constitutive expression promoter double CaMV35S、E12 and inducible expression promoter rd29A.
     构建了分别由双35S 组成型启动子、E12 强组成型启动子和rd29A 诱导型启动子调控的核糖体失活蛋白基因、几丁质酶基因和DREB1A 基因的多价基因植物表达载体;
短句来源
     Using filling-gap method,a hybrid gene named PfSI coding for several epitopes of Pfs230 and Pfs48/45 in the sexual stage of Plasmodium falciparum and a exogenous T cell epitope from interleukin-I(IL-I)was designed and chemically synthesized.
     采用缺口填补法将恶性疟原虫有性期Pfs230和Pfs48/45抗原中的2个T细胞表位,3个B细胞表位以及人白介素-Ⅰ中的一个T细胞激活位点按预设方案,拼接成复合多价基因PfSI。
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  相似匹配句对
     Multivariate GARCH Models on the Relationship between Price and Trading Volume
     量关系的元GARCH建模
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     Construction of Multivalent DNA Vaccine against Schistosoma japonicum
     日本血吸虫DNA疫苗的构建
短句来源
     Multiple target Tracking
     目标跟踪
短句来源
     TRACKING OF MULTIPLE TARGETS
     目标跟踪
短句来源
     Objective To study preparation of polyvalent DNA vaccine and the control of multiple gene expression.
     目的为了便于DNA疫苗的制备和基因表达的研究。
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  multiple genes
Simultaneous introduction of multiple genes into plants is a critical step in plant genetic engineering to manipulate multiple functional genes in metabolic engineering and trait stacking.
      
Bound to DNA in the cell nucleus, YB-1 functions as a transcription factor interacting with inverted CCAAT-box (Y-box) in promoters and enhancers of multiple genes.
      
The most probable scheme of origination of the multiple genes of the POLR2J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated.
      
A new method of preparing fiber-optic DNA biosensor and its array for the simultaneous detection of multiple genes is described.
      
Involvement by multiple genes in complex diseases usually occurs but the isolation and identification of specific genes so far has been exceptional.
      
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A 274 bp gene of human plasmodium falciparum hybrid peptide antigen,which isdivided into 8 oligonucleotide fragments(F1~F8),has been synthesized by the solid-phase phos-phoramidite methods with ABI-381A DNA Synthesizer.The gene encodes several T-or B-cellepitopes of CSP,RESA,MSA1 and MSA2.F1,F3,F5,and F7 are located in the positive chain, andF2,F4,F6F8 in the negetive one.Each two neighbouring fragments(i.e ,F1 and F2 ,or F2 and F3)has 15 matching bases.After being annealed and ligated with T4 DNA ligase,the...

A 274 bp gene of human plasmodium falciparum hybrid peptide antigen,which isdivided into 8 oligonucleotide fragments(F1~F8),has been synthesized by the solid-phase phos-phoramidite methods with ABI-381A DNA Synthesizer.The gene encodes several T-or B-cellepitopes of CSP,RESA,MSA1 and MSA2.F1,F3,F5,and F7 are located in the positive chain, andF2,F4,F6F8 in the negetive one.Each two neighbouring fragments(i.e ,F1 and F2 ,or F2 and F3)has 15 matching bases.After being annealed and ligated with T4 DNA ligase,the 8 fragments wereinterlinked into a double DNA chain with 8 gaps. Then ,those gaps were filled with the gene am-plification methods. The technique,in combination with genetic chemical splicing and gene ampli-fication in vitro,is named as “Filling-Gap Method”by us.Analysis of PCR or DNA sequencingshows that the filling-gap method is cheaper,simpler and more effective than other methods inrecombing gene.(Fig.1-6)

将恶性疟原虫保护性抗原环子孢子蛋白(CSP)、环状体感染红细胞表面抗原(RESA)、裂殖子表面抗原1和2(MSA1和MSA2)4种抗原中具有T和/或B细胞表位的6个基因片段,按照我们设计的方案排成一条线性序列,经在计算机上分析比较后,分成长短不一的8个基因片段(F1~F8),其中F1、F3、F5和F7为正链片段,F2、F4、F6和F8为负链片段。每相邻两个片段间预设15个互补碱基搭头。在DNA合成仪上分别合成这8个片段后,把F2~F76个片段分别磷酰化,按等摩尔浓度混合。经30℃退火,使8个片段连接起来,然后按基因体外扩增的原理,填补互补碱基以外的缺口,重组成新的多价基因。我们把这种化学拼接法取名为“缺口填补法(Filling-qapMethod)”,经克隆和测序验证,表明这种方法具有效果好、经济、简便等优点。

Using filling-gap method,a hybrid gene named PfSI coding for several epitopes of Pfs230 and Pfs48/45 in the sexual stage of Plasmodium falciparum and a exogenous T cell epitope from interleukin-I(IL-I)was designed and chemically synthesized. The gene was composed of 147 base pairs and coded for a 43 amino acid peptide. After purification,the target gene PfSI was inserted into plasmid pcDNA3 at poly linker.Then,the recombinant vector pcDNA3/PfSI was transformed into E.coli JM109. Positive clones were screened...

Using filling-gap method,a hybrid gene named PfSI coding for several epitopes of Pfs230 and Pfs48/45 in the sexual stage of Plasmodium falciparum and a exogenous T cell epitope from interleukin-I(IL-I)was designed and chemically synthesized. The gene was composed of 147 base pairs and coded for a 43 amino acid peptide. After purification,the target gene PfSI was inserted into plasmid pcDNA3 at poly linker.Then,the recombinant vector pcDNA3/PfSI was transformed into E.coli JM109. Positive clones were screened by ampicillin and PCR technique,and identified with restriction endonucleases. The success of recombination of the hybrid gene PfSI will facilitate the high level expression of transmission-blocking antigens of Plasmodium falciparum.

采用缺口填补法将恶性疟原虫有性期Pfs230和Pfs48/45抗原中的2个T细胞表位,3个B细胞表位以及人白介素-Ⅰ中的一个T细胞激活位点按预设方案,拼接成复合多价基因PfSI。复合基因全长147bP,编码43肽复合抗原,经分离、纯化后,插入质粒pcDNA3的多克隆位点,构建重组载体pcDNA3/PfSI。再将重组载体转化大肠杆菌JM109,用氨苄青霉素和PCR扩增法筛选阳性克隆,最后用限制性内切酶对阳性克隆进行鉴定。复合基因PfSI拼接、克隆的成功为在体外高效表达恶性疟原虫传播阻断抗原创造了条件。

In order to provide α gene material for prepare the gene engineering vaccine against Clostridium perfringens, β toxin gene was amplificated from genomic DNA of Clostridium perfringens type B by polymerase chain reaction(PCR) PCR product was cleaved with restriction endonucleases Bam HI and Eco RI,and inserted into vector pET 28C(+) directively,Which had been cleaved with Bam HI and Eco RI.The recombinant plasmid pECB2 was identified by restriction endonuclease analysis,PCR amplification test and nucleotide...

In order to provide α gene material for prepare the gene engineering vaccine against Clostridium perfringens, β toxin gene was amplificated from genomic DNA of Clostridium perfringens type B by polymerase chain reaction(PCR) PCR product was cleaved with restriction endonucleases Bam HI and Eco RI,and inserted into vector pET 28C(+) directively,Which had been cleaved with Bam HI and Eco RI.The recombinant plasmid pECB2 was identified by restriction endonuclease analysis,PCR amplification test and nucleotide sequencing.The results showed that β toxin gene fragment of 930 bp have been cloned with a positive reading frame.

为了给产气荚膜梭菌(Clostridiumperfringens)β毒素基因工程亚单位苗和细菌毒素多价基因工程苗的研制提供基因材料,用聚合酶链式反应(PCR)技术,从B型产气荚膜梭菌染色体基因组中扩增了930bp的β-毒素基因。用限制性核酸内切酶BamHI和EcoRI对PCR产物进行双酶切处理,然后通过T4DNA连接酶将其定向连接于事先经同样的双酶切处理的载体质粒pET-28C(+)的多克隆位点,转化至受体菌BL21(DE3)中。经BamHI和EcoRI双酶切分析和PCR扩增检测,证明重组质粒pECB2中含有产气荚膜梭菌的β-毒素基因。经核苷酸序列分析,明确了克隆的β-毒素基因在重组质粒中的连接向位和阅读框架是正确的。

 
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