The results showed that the complete cDNA was 1263bp in length, including 42bp of 5' untranslatable region, 168bp of 3' untranslatable region which contained a polyadenylation signal, AATAAA, and a 24bp polyadenylation tail.
Objective:To check if mutated polyadenylation signal retroviruses can produce viral host readthrough transcripts (Rth) and have the ability to transform normal REF 1 cells.
Objective To verify if mutated polyadenylation signal retroviruses can produce viral-host readthrough transcripts (Rth) and have the ability to transform human gastric epithelial GES-1 cells, and to discuss the new functions of retroviruses in gastric cancer related gene research.
The results showed that the complete cDNA was 1 984 bp in length, including 16 bp of 5′ untranslated region,202 bp of 3′ untranslated region which contained a polyadenylation singal, AATAAA, and a 17 bp polyadenylation tail. The coding region encoded a peptide of 588 amino acid residues.
Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
Northern blot analysis of viral RNA from infected pools and individual G418-resistant clones demonstrated that mutation of consensus LTR polyadenylation signals generated Rth viral RNA in the infected GES-1 cells. Phenotypic analysis results showed that the GES-1 cells infected with plyadenylation signal mutant viruses tended to grow in a cluster manner.
Sequences corresponding to a polyadenylation signal and a poly(A) attachment site were found immediately downstream from the 3' end of the hemA open reading frame.