1.The Expression of Human Macrophage Metalloelastase in Gastric Cancer Cell Lines and Gastric Cancer Tissues and Its Clinical Significance 2.The Study of the Associalation between DPYD Gene Polymorphism and Metabolism of 5-FU and the Clinical Signifi
Results: In gastric cancer tissues, the positive expression rate of CD44V6 mRNA, MMP 9 mRNA and P53 protein was 60% , 63% and 65% , respectively. In tissues with positive expression of CD44V6 mRNA, MMP 9 mRNA and P53 protein, lymphnode metastasis rate was 92% , 92% and 90% , respectively, significantly higher than tissues with negative expression(38% , 32% and 33% , P<0.05).
The apoptotic index in the gastric cancer tissue with positive expression of hMLH_1 [(3.652±0.241)%] was higher than that in the gastric cancer tissue with negative expression of hMLH_1 [(1.887±0.212)%](P<0.01).
Reversal of multidrug resistance of gastric cancer cells by downregulation of CIAPIN1 with CIAPIN1 siRNA
The overexpression of a new cytokine-induced apoptosis inhibitor 1 (CIAPIN1) gene has been shown previously to promote a multidrug resistant phenotype in gastric cancer cells through the upregulation of MDR1 and MRP1.
CIAPIN1 siRNA could significantly downregulate the expression of Bcl-2, and upregulate the expression of Bax, but does not alter the expression of PTEN in gastric cancer cells.
These observations suggested that the siRNA constructs of CIAPIN1 we obtained could effectively downregulate the expression of CIAPIN1 and reverse the resistant phenotype of gastric cancer cells.
Further study of the biological functions of CIAPIN1 may be helpful for understanding the mechanisms of multidrug resistance of gastric cancer and in developing possible strategies to treat gastric cancer.
The allelic distribution ofEcoRI andBamHI fragments ofras family genes between the human primary gastric cancer tissues and the corresponding adjacent normal tissues did not show any differences.
Four protooncogenes, c-H-ras, N-ras, c-myc and c-fos, were found to be transcriptionally active in the gastric cancer tissues in some cases examined.
AI and PI in gastric cancer tissues were (6.5 ± 3.7) % and (49.8 ± 15.9) % respectively, and the rate of AI/PI was 0.13 ± 0.05, which were obviously different from those of normal gastric mucosa in paragastric cancer (P >amp;lt; 0.01).
There was significant difference in them between the gastric cancer tissues and normal gastric mucosa in pericarcinoma in TNM stageI to M (P >amp;lt; 0.05).
In this study, we evaluated the protein expression level of RhoC in gastric cancer tissues and cell lines.
Objective: To determine the prevalence of Mycoplasma hyorhinis in archived paraffin-embedded gastric cancer tissue.
Methods: The expression of MMP-2, MMP-9 and MVD were detected by immunohistochemistry SP method on 65 cases of gastric cancer tissue and 32 adjacent gastric mucosa.
Specimens of gastric ulcer had significantly lower enzyme activities of GSH-peroxidase and GSH-aryltransferase, whereas gastric cancer tissue had significantly lower concentrations of total glutathione.
Immunocytochemical and immunohistochemical staining confirmed that phage IN11 could specifically bind to Co-HUVECs as well as vascular endothelial cells in gastric cancer tissue sections.
Gastric cancer tissue microarray was formed and the expression of survivin and STAT1 in gastric cancer was detected by immunohistochemical method.