Study in vitro suggested that ooexpression of Naf1 site-specific mutants(Q 463→E 463 ,Q 464→E 464) interferes in a dominant negative manner with the NF-κB inhibiting function of wild type Naf1.To test the role of Naf1-mut in vivo we generate the pCMV- Tag 3B/Naf1-mut transgenic mouse .
体外试验表明Naf1的突变Q 463→E 463 ,Q 464→E 464(Naf1-mut)和野生型的Naf1共转细胞时能够破坏Naf1对NF-κB激活的抑制作用。 为了在体内水平验证上述结论我们构建了pCMV- Tag 3B/Naf1-mut转基因小鼠。
This has provided a basis to establishing the HCV5′NCR transgene mouse model of liver specific expression. It is of great significance to evaluate the antisense drugs against HCV and liver targeted delivery systems.
The liver of the transgene mice resembled the acute hepatitis B.The early phase of injury(2nd d and 4th d) was a few lymphomononuclear cells infiltrate on portal areas and sinusoid. Hepatocytes began to degenerate on 4th day.
A study of recombinant human lactoferrin secreted in milk of transgenic mice
Analysis of the maintenance of pCI.ARS-neo and pCI.ori-neo in transgenic mice and in cultured immortalized human T cells revealed the interplay of transcription and replication, as the CMV element suppressed autonomous replication of both plasmids.
Examples are given for utilization of conditionally immortal cells obtained in vivo from tissues of transgenic mice and rats carrying the gene of mutant T-antigen tsA58 SV40.
Analysis of transfer of microinjected zygotes in production of transgenic mice
The data on transfer of mouse eggs microinjected with DNA during production of transgenic mice were analyzed.
The cardioprotective role of Trx 1 is further confirmed with transgenic mouse hearts overexpressing Trx 1.
A total of four transgenic mouse lines were obtained, in three of which the vector was integrated into genes active during embryogenesis.
To study the mechanisms of such instability, two transgenic mouse lines and 11 clones of transfected F9 mouse embryonic teratocarcinoma cells were obtained with the 3.8-kb repetitive unit (Sat) of Bos taurus satellite DNA IV.
G-CSF of transgenic mouse milk was shown to stimulate the formation and growth of granulocyte-containing colonies in human umbilical blood cell culture and be close or identical in physiological activity to the natural human G-CSF.
These observations are associated with an increased atrial vulnerability.????The Cx40 deficient mouse provides a good example of the relevance of transgenic mouse models to clarify the mechanisms of arrhythmogenesis.
EGb761 significantly improved motor performance and survival, and protected against a loss of spinal-cord anterior motor horn neurons in male G93A mutant transgenic ALS mice, but not in littermate female mutant transgene mice.
A variety of airway abnormalities were noted in the transgene mice.
Lungs were obtained from CC10-IL-13 transgene mice and transgene littermate controls.