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dnadna杂交
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  dna-dna hybridization
     Applied DNA-DNA hybridization in determination of oral Streptococcus
     DNA-DNA杂交技术在口腔链球菌鉴定中的应用
短句来源
     c. pv. campestris and cloned in the vector pBR322. The results of DNA-DNA hybridization and Tn5 mutagenesis analysis show that there is only one copy of lysyl-tRNA gene in Xoo genome and mutation of the gene is fatal to Xoo.
     以32P标记的甘蓝黑腐病黄单胞菌赖氨酰-tRNA合成酶基因为探针,将水稻白叶枯病黄单胞菌同功的基因定位于pGXN30001.6kb的BamHI片段上.通过DNA-DNA杂交,标记置换等方法初步证实:在稻白叶枯病黄单胞菌中只有一个拷贝的赖氨酰-tRNA合成酶基因,该基因失活对病菌是致死的。
短句来源
  dna-dna hybridizations
     Using DNA-DNA hybridizations, nif clones of Frankia pCclGX from Co01,pAt1GX from At4, pHr18GX and pHr11-③ GX from Hr18 were isolated.
     利用DNA-DNA杂交方法,分离细枝木麻黄的Frankia菌株Co01的nif克隆pCc1GX,赤杨内生菌株At4的nif克隆pAt1GX及沙棘的FrankiaHr18的nif克隆pHr18GX、pHr11-③GX。
短句来源
  “dna-dna杂交”译为未确定词的双语例句
     The DNAs homology for Jb 2 and Jb 3 strains, Jb 2 and Tb 1 strains, Jb 2 and E 4 strains were carried out by liquid phase reassociation rate method.
     应用DNA-DNA杂交技术(初始复性速率法)比较了1型鸭疫里氏杆菌Jb2与Jb3株之间以及Jb2与Tb1株、E4株之间的同源性。 结果表明,Jb2与Jb3之间的DNA同源值为68.63%;
短句来源
     In this study,the whole DNA of L.biflexa sv Patoc strain Patoc 1 was labelled with photohiotin. It was used as a probe to detect different serovars of L. interrogans,and L. biflexa sv Andamana Strain CH11 by the dot-hybridization method on a nintrocellulose membrane and the microtiter plate method.
     本文以光生物素标记的双曲钩体Patoc1株全DNA作为探针,对不同血清型的问号钩体与双曲钩体在硝酸纤维素膜上和微量反应板中进行DNA-DNA杂交试验。
短句来源
     Some technique problems are discussed. Such as purification,precision of DNA and temperature as well as time of DNA hybridization and standard of judgement.
     本文还就DNA-DNA杂交技术中的某些技术关键问题,包括DNA的提取和精度,纯度要求,杂交的时间和温度以及DNA同源率的判断标准进行了讨论。
短句来源
     This paper reports on the basis of a large amount of literatures,introduces the basic concepts,principles,characteristics,and their application at the present and in the future on taxonomy of rhizobia of some new molecular biological methods such as whole cell protein electrophoresis,multilocus enzyme electrophoresis (MLEE),DNA G+C mol%,DNA DNA hybridization,restriction fragment length polymorphism (RFLP),randomly amplified polymorphic DNAs (RAPD),repetitive extragenic palindrome PCR (REP PCR),16s rDNA sequencing.
     研究在参考大量文献的基础上,概述了全细胞可溶性蛋白电泳、多位点酶电泳、DNAG+C摩尔百分含量测定及DNA-DNA杂交、限制性片段长度多态性分析、随机扩增DNA片段的多态性、基因外回文重复序列PCR扩增技术及16SrDNA序列分析确定根瘤菌系统发育等7种分子生物学方法的原理、特点,以及在根瘤菌分类研究中的应用现状和前景。
短句来源
     The results of RFLP were chiefly accorded with those of numerical taxonomy, SDS-PAGE analysis and DNA-DNA hybrization.
     聚类结果与数值分类、全细胞蛋白电泳分析及DNA-DNA杂交结果基本一致。
短句来源
  相似匹配句对
     DNA,respectively.
     DNA
短句来源
     DNA Sequencing by hybridization
     DNA杂交测序法
短句来源
     Superoxide dismutase could not inhibite DNA damage in the xanthine-xanthine oxidase system.
     DNA
短句来源
     Study on interaction between nitrophenols and DNA in hybridization
     对硝基苯酚与杂交DNA的相互作用
短句来源
     e-Hybrid Rice
     e杂交
短句来源
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  dna-dna hybridization
Based on the 16S rRNA gene sequences, phenotypic characteristics, and the results of DNA-DNA hybridization, the taxonomic affiliation of the isolates was tentatively established.
      
The taxonomic investigation of five streptomycete cultures belonging to the International Streptomyces Project (ISP) standards was carried out using the methods of population analysis, DNA-DNA hybridization, and multilocus DNA fingerprinting.
      
The Role of DNA-DNA Hybridization and 16S rRNA Gene Sequencing in Solving Taxonomic Problems by the Example of the Order Haloana
      
The phylogenetic relationships of 12 aerobic dichloromethane-degrading bacteria that implement different C1-assimilation pathways was determined based on 16S ribosomal RNA sequences and DNA-DNA hybridization data.
      
The level of DNA-DNA hybridization of the isolate (strain 101T) and Desulfacinum infernum(strain BαG1T) is as low as 34%.
      
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  dna-dna hybridizations
Example data from elm (Ulmus procera) SR4 regenerant plants, shown to be genetically modified by PCR and DNA-DNA hybridizations, in which higher GUS expression levels are found in stems than in leaves demonstrates the utility of this approach.
      
The results of multiple DNA-DNA hybridizations indicated that separate species designations were appropriate for H.
      
DNA-DNA hybridizations demonstrated that multiple homologues of IS4712 were also present within the genomes of several other thermophilic bacillus isolates.
      
From the results of DNA-DNA hybridizations, strain TE26T was genetically coherent to strain K6 (94 and 88% hybridization), and exhibited lower relatedness to R.
      
heterostrophus mtDNA was constructed using the restriction enzymes BamHI, EcoRI, and PvulI by DNA-DNA hybridizations with cloned or purified mtDNA BamHI fragments.
      
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A strain of Haloalkalophilic Archaeon Y21 was isolated from soda lake in Inner Mongolia Autonomous Region. The isolate is rod shaped, motile and red clone. On the basis of morphology, physiology and biochemistry, polar lipid compositiion, phylognetic analysis and DNA\|DNA hybridization, we found that strain Y21 is new species of the genus \%Natrilba\%. The name \%Natrilba wudunaoensis\% sp.nov.is proposed.

从内蒙古乌都淖咸性盐湖分离到嗜盐碱菌菌株Y21。其形态为杆状,能运动,红色菌落,通过生理生化、极性脂成分、基于16SrRNA序列的系统发育学分析和DNADNA杂交同源性比较,发现菌株Y21是Natrilba属中一个与其它成员不同的新种,命名为Natrilbawudunaoensissp.nov.。

Objective: Using heat-resisting alkaline phosphatase to establish the method of microplate quantitative DNA-DNA hybridization for examining the homology among bacterial strains with high G+C percent. Methods: The denatured bacterial chromosome DNA linked to microplate reacted to the probe which was labeled by photobiotin. After adding streptoavidin labeled heat-resisting alkaline phosphatase, the enzyme was bond to microplate through combination of streptoavidin and photobiotin. The results of hybridization...

Objective: Using heat-resisting alkaline phosphatase to establish the method of microplate quantitative DNA-DNA hybridization for examining the homology among bacterial strains with high G+C percent. Methods: The denatured bacterial chromosome DNA linked to microplate reacted to the probe which was labeled by photobiotin. After adding streptoavidin labeled heat-resisting alkaline phosphatase, the enzyme was bond to microplate through combination of streptoavidin and photobiotin. The results of hybridization could be read by fluorescence analyzer following the relevant substrate was added. Results: The favorable hybridization results could be obtained when 1 μg/well covered DNA and 25~50 ng/well probe was used respectively, and pH 9.5 Tris-HCl buffer was the optimum condition for reaction of enzyme and substrate. The results showed that the method was sensitive, specific and simple, and it will adapt to determine the homology of bacteria with high G+C content. Conclusion: It had not obviously influence over high temperature of hybridization and determination when the heat-resisting alkaline phosphatase was used for quantifing DNA-DNA similarity value among bacteria, especially in Mycobacterium and Pseudomonas possessed high G+C content. [

目的应用耐热碱性磷酸酶(AKP)于微孔板DNADNA杂交技术,建立高G+C含量细菌染色体DNA同源性测定的特异性方法,为此类细菌的分类与鉴定奠定基础。方法细菌染色体DNA经分离纯化并变性后,直接非共价结合于固相微孔板中,与标有光标生物素的DNA探针进行杂交。借助抗生物素蛋白与生物素的特异性结合,通过与板上带有生物素的杂交体作用,使得链霉亲和素连接的耐热AKP固定于板上,根据底物加入后的酶促反应强度,定量判读杂交结果。结果微孔板中1μg/孔DNA的包被浓度与25~50ng/孔的生物素探针呈现较为理想的杂交结果,pH95TrisHcl缓冲液系耐热AKP的最佳作用条件。应用该法于高G+C含量的分枝杆菌种间染色体同源性分析表明,方法具有敏感、特异、简便等优点。结论耐热AKP应用于微孔板定量杂交,可不受较高杂交和测定温度的影响,适用于高G+C含量的结核杆菌等分枝杆菌属以及假单胞菌属的定性检测和同源性的定量分析。

Strain AD9--an aniline-degradation bacterium was isolated from the dying plant soil. AD9 has resistance to Amp, Km, Rif and has no Chloromycetin and Tc. The temperature for AD9 to grow and degrade aniline was 20~37℃,and the optimum temperature was 30℃. The strain AD9 grew well in the pH scale range from 4.0 to 9.0. The optimum pH was 7.0. The strain AD9 grew well in the aniline concentration range from 200 mg/L to 4500 mg/L. The best aniline concentration was 1000mg/L. The 16SrRNA of strain AD9 belongs to the...

Strain AD9--an aniline-degradation bacterium was isolated from the dying plant soil. AD9 has resistance to Amp, Km, Rif and has no Chloromycetin and Tc. The temperature for AD9 to grow and degrade aniline was 20~37℃,and the optimum temperature was 30℃. The strain AD9 grew well in the pH scale range from 4.0 to 9.0. The optimum pH was 7.0. The strain AD9 grew well in the aniline concentration range from 200 mg/L to 4500 mg/L. The best aniline concentration was 1000mg/L. The 16SrRNA of strain AD9 belongs to the evolution branch of Delftia. The G+C content of AD9 is 66.8 mol%, which is very similar to that of D. tsuruhatensis T7. In addition, the hybridization rate between AD9 and T7 was 83.8%. Together with the physiological and biochemical tests, the strain is named Delftia tsuruhatensis AD9.

从活性污泥中分离到一株能以苯胺为唯一碳源、氮源生长的苯胺降解菌株AD9,该菌株最适生长的苯胺浓度为1000mg/L,降解效率可达90%,对苯胺耐受程度高达4500mg/L,降解苯胺的最适pH值为7.0,最适温度为30℃。以上实验结果表明,AD9具有降解苯胺速率快、耐受苯胺浓度高的特性。该菌具有氨苄青霉素、卡那霉素和利福平抗性,无氯霉素、四环素抗性,其16SrDNA序列与多株Delftiasp.菌有很高的同源性,其G+C含量为66.8mol%,非常接近于标准菌株D.tsuruhatensisT7(66.2mol%)。另外该菌和T7的DNADNA杂交率为83.8%。结合AD9的表型鉴定将该菌株归属为D.tsuruhatensisas。这是D.tsuruhatensis菌种苯胺降解细菌菌株的首次报道。

 
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