This paper used the protoplast fusion technique between the respiratory deficient mutant(rho-)of the high yielding ethanol yeast sb724 and 1300(his-)& thermoduric yeast yw(Asn-)to abtain two stable uninucleate fusants F16 & F19 induced by electrical field.
The results showed that there were certain polymorphic DNA fragments between them and some of these fragments might be correlated with thermotolerant trait.