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柔性固定化酶
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  相似匹配句对
     Studies on Immobilization of Enzyme and Cells
     和细胞的固定化研究
短句来源
     Review of current research on immobilized enzyme
     固定化研究进展
短句来源
     Study on Flexible Immobilization Papain
     木瓜蛋白柔性固定化研究
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     Flexible Immobilized Papain on the Chitosan Support
     壳聚糖载体柔性固定化木瓜蛋白
短句来源
     Flexible immobilization of papain on amino polystyrene support
     胺化聚苯乙烯载体柔性固定化木瓜蛋白
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Flexible immobilized enzyme" model is proposed,that is: the flexible carrier is prepared by linking a hydrophilic and flexible polymer chain to the suface of the support,then enzyme is connected on the carrier by covalent bond Flexible immobilization have some following characteristics: it can meliorate disadvantage of enzyme inactivity caused by direct immobilization or by arm spacer immobilization and improve free degree of immobilized enzyme; monodisperse micro-spheres as support can profit homogenization...

Flexible immobilized enzyme" model is proposed,that is: the flexible carrier is prepared by linking a hydrophilic and flexible polymer chain to the suface of the support,then enzyme is connected on the carrier by covalent bond Flexible immobilization have some following characteristics: it can meliorate disadvantage of enzyme inactivity caused by direct immobilization or by arm spacer immobilization and improve free degree of immobilized enzyme; monodisperse micro-spheres as support can profit homogenization in the immobilization reaction and enzyme catalysis reaction Dialdehyde starch as a flexible chain is connected on the carboxylized polystyrene support,flexible carrier is obtained and then papain is immobilized on this flexible carrier The activity recovery of immobilized papain can reach 50% equal to twice activity recovery of arm spacer immobilized papain with glutaric aldehyde

提出“柔性固定化酶”的模型 ,即 :用一亲水、柔性高分子链接枝于载体表面制得柔性固定化载体 ,再用其以共价键合的方式进行酶的柔性固定化。其特点是 :柔性固定可改善因直接固定化及手臂固定化使酶失活的缺陷 ,并提高固定化酶的自由度 ;如选用粒径单分散微球可改善固定化反应及固定化酶催化反应的均一性。以双醛淀粉 (DAS)为柔性链对羧基化聚苯乙烯载体进行柔性化修饰后 ,固定木瓜蛋白酶 ,其活力回收率可达 5 0 % ,相当于用戊二醛进行手臂固定化的活力回收率的 2倍

In this paper the flexible immobilization model of enzyme is presented.A new amino polystyrene (0.4—6.0 mmol NH_2·g -1) was prepared by Mannich reaction as immobilization support, DAS was used as the flexible chain,and then papain was immobilized. The recovery of immobilized enzyme activity could reach 40%—50%,equal to 1.8—2.4 times that of arm spacer support, and the stability of immobilized enzyme was good.The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional...

In this paper the flexible immobilization model of enzyme is presented.A new amino polystyrene (0.4—6.0 mmol NH_2·g -1) was prepared by Mannich reaction as immobilization support, DAS was used as the flexible chain,and then papain was immobilized. The recovery of immobilized enzyme activity could reach 40%—50%,equal to 1.8—2.4 times that of arm spacer support, and the stability of immobilized enzyme was good.The flexible immobilized model could achieve a higher recovery of immobilized enzyme activity than traditional covalent immobilization and arm spacer immobilization.

提出酶的“柔性固定化”模型,并以Mannich反应得到担载量为0.4~6.0mmolNH2·g-1的胺化聚苯乙烯树脂为载体,以双醛淀粉为柔性链,对木瓜蛋白酶进行柔性固定化,酶活回收率可达40%~50%,相当于手臂固定化酶活力回收率的1.8~2.4倍,且柔性固定化酶稳定性较好.该结果说明,酶的“柔性固定化”模型可以改善传统共价结合固定化酶及手臂固定化酶活力回收率不高的缺陷.

 
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