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肾近球小管
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     The Ultrastructures of Pig Kidney Proximal Tubule
     猪小管的超微结构
短句来源
     THE ULTRAMICROSCOPIC STRUCTURE OF THE RENAL GLOMERULUS AND PROXIMAL TUBULES OF CYPRINUS CARPIO
     鲤鱼小体和小管的电子显微镜观察
短句来源
     Primary Tissue Culture of Proximal Tubular Epithelial Cells in Human
     人小管上皮细胞的原代培养
短句来源
     Objective To observe the ultrastructrues of renal proximal tubule in pig kidney.
     目的 研究猪小管的超微结构。
短句来源
     The CB was mainly expressed in proximal convoluted tubular epithelial cells and was less in golmerulus, distal convoluted tubule and collecting duct.
     CB主要在小管上皮细胞内表达;
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  kidney proximal tubule
NHK-C cultures are morphologically heterogeneous but exhibit, predominantly, the functional characteristics of cells of the kidney proximal tubule.
      
We have characterized primary rabbit kidney proximal tubule cells grown on Millipore cellulose filters (Millicell-HA) in which the cells are exposed to culture medium on both the apical and basolateral sides.
      
Use of the pH-sensitive dye BCECF to study pH regulation in cultured human kidney proximal tubule cells
      
This protocol describes the use of the pH-sensitive, intracellularly trapped dye 2',7'-bis (2-carboxyethyl), 5 (and -6) carboxyfluorescein (BCECF), to characterize the pH regulating mechanisms in cultured human kidney proximal tubule cells.
      
The pattern of LI increase was unique to each tissue with liver (median and lateral lobes) increased two-fold on day 3 and returning to control levels thereafter while kidney proximal tubule LI rose gradually with time and remained elevated on day 7.
      
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The characteristics of ATP-driven proton and osmotic water transport werestudied in endocytic vesicles isolated from rat kidney proximal tubule labelled invivo with fluorescein isothiocyanate-dextran(FITC-dextran).ATP-driven protontransport was measured from the time course of endosome PH following additionof external ATP.The rate of endosome acidification and the minimum pH weredependent on the ATP concentration.At an initial endosome pH of 7.4,the finalpH values were 7.30,6.99,6.68,6.38 and 6.39 at[ATP]= 0.005,0.05,0.5,5and...

The characteristics of ATP-driven proton and osmotic water transport werestudied in endocytic vesicles isolated from rat kidney proximal tubule labelled invivo with fluorescein isothiocyanate-dextran(FITC-dextran).ATP-driven protontransport was measured from the time course of endosome PH following additionof external ATP.The rate of endosome acidification and the minimum pH weredependent on the ATP concentration.At an initial endosome pH of 7.4,the finalpH values were 7.30,6.99,6.68,6.38 and 6.39 at[ATP]= 0.005,0.05,0.5,5and 10 mmol/L,respectively.The acidification was inhibited by 97% at 0.5mmol/L N-cthylmaleimide but was not affected by vanadate and oligomycin.Osmotic water permeability was determined in the same endosomes from the rapidknetics of FITC-dextran fluorescence following an inward sucrose gradient.The osmotic water permeability coefficient was 0.03 cm/s at 23℃.Water perme-ability was inhibited by 70% with addition of 0.5 mmol/L mercuric chloride.Theinhibition was reversed completely by adding 5 mmol/L mercaptoethanol.Thesedata demonstrate that proximal tubule endosomes contain a proton ATPase andwater channel.The endocytic process may be important for regulation of acid-ification and fluid resorption in the proximal tubule.

本实验采用异硫氰酸-葡聚糖荧光素(fluorescein isothiocyanate-dextran,FITC-dextran)体内标记法,研究大鼠肾近球小管细胞胞饮体(endosome)膜上 H~+-ATP 酶的活性及水的渗透性转运。通过观察在胞饮体外加入一定量 ATP 后,胞饮体内 pH 值的时间反应曲线,从而测定 ATP-依赖的 H~+在胞饮体膜上的转运情况。胞饮体内的酸化速度及 pH 的最低值与加入的 ATP 浓度有关。在加入 ATP 前,胞饮体内的 pH 值为7.4,加入不同浓度的 ATP 后,即[ATP]为0.005,0.05,0.5,5和10mmol/L,胞饮体内 pH 最低值分别为7.30,6.99,6.68,6.38和6.39。此种由 ATP 引起的酸化反应,被0.5mmol/L N-ethylmaleimide(NEM)抑制97%,但不被钒酸盐和 oligomycin 所抑制。实验还同时观察了此种胞饮体水的渗透性转运机制。通过在胞饮体膜内外建立一个蔗糖浓度梯度。观察 FITC-dextran 荧光信号的快速动力学变化过程,从而测定由于渗透压梯度引起的水在胞饮体膜上转运的特征。...

本实验采用异硫氰酸-葡聚糖荧光素(fluorescein isothiocyanate-dextran,FITC-dextran)体内标记法,研究大鼠肾近球小管细胞胞饮体(endosome)膜上 H~+-ATP 酶的活性及水的渗透性转运。通过观察在胞饮体外加入一定量 ATP 后,胞饮体内 pH 值的时间反应曲线,从而测定 ATP-依赖的 H~+在胞饮体膜上的转运情况。胞饮体内的酸化速度及 pH 的最低值与加入的 ATP 浓度有关。在加入 ATP 前,胞饮体内的 pH 值为7.4,加入不同浓度的 ATP 后,即[ATP]为0.005,0.05,0.5,5和10mmol/L,胞饮体内 pH 最低值分别为7.30,6.99,6.68,6.38和6.39。此种由 ATP 引起的酸化反应,被0.5mmol/L N-ethylmaleimide(NEM)抑制97%,但不被钒酸盐和 oligomycin 所抑制。实验还同时观察了此种胞饮体水的渗透性转运机制。通过在胞饮体膜内外建立一个蔗糖浓度梯度。观察 FITC-dextran 荧光信号的快速动力学变化过程,从而测定由于渗透压梯度引起的水在胞饮体膜上转运的特征。在230℃时,水的渗透性通透系数(osmotic water permeability coefficient,P_f)为0.03cm/s;加入0.5mmol/L HgCl_2后,水的转运被抑制70%。此抑制反应可被5mmol/L 巯基乙醇(β-Mcrcaptoethanol)完全逆转。上述结果提示:大鼠肾近球小管胞饮体膜含有H~+-ATP 酶和水的转运通道。胞饮?

 
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